Construction of non-polar mutants in Haemophilus influenzae using FLP recombinase technology

BMC Molecular Biology

Volumn 9, Issue , 2008, Pages

  Tracy, Erin ^a   Ye, Fang ^a   Baker, Beth D ^a,b   Munson Jr , Robert S ^a,b  

^a NATIONWIDE CHILDREN S HOSPITAL   (United States)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID VECTOR; RECOMBINASE; SPECTINOMYCIN; STREPTOMYCIN; DNA POLYMERASE; FIMBRIA PROTEIN; VIRUS PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOTECHNOLOGICAL PROCEDURES; ELECTROPORATION; GENE CASSETTE; GENE CONSTRUCT; GENE EXPRESSION; GENE MUTATION; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; HAEMOPHILUS INFLUENZAE; MUTANT; NONHUMAN; NONTYPEABLE HAEMOPHILUS INFLUENZAE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REPLICON; BACTERIOPHAGE LAMBDA; BACTERIUM TRANSFORMATION; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; METABOLISM; MUTAGENESIS; MUTATION; OPERON;

HAEMOPHILUS INFLUENZAE; NEGIBACTERIA; PILA;

BACTERIOPHAGE LAMBDA; DNA NUCLEOTIDYLTRANSFERASES; FIMBRIAE PROTEINS; GENETIC ENGINEERING; HAEMOPHILUS INFLUENZAE; MUTAGENESIS; MUTATION; OPERON; RECOMBINATION, GENETIC; TRANSFORMATION, BACTERIAL; VIRAL PROTEINS;

EID: 58349103721     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-9-101     Document Type: Article

References (26)

1. Kilpi T Herva E Kaijalainen T Syrjanen R Takala AK Bacteriology of acute otitis media in a cohort of Finnish children followed for the first two years of life Pediatr Infect Dis J 2001, 20(7):654-662. 10.1097/ 00006454-200107000-00004 11465836
2. Murphy TF Respiratory infections caused by non-typeable Haemophilus influenzae Curr Opin Infect Dis 2003, 16(2):129-134. 12734445
3. Craig L Pique ME Tainer JA Type IV pilus structure and bacterial pathogenicity Nat Rev Microbiol 2004, 2(5):363-378. 10.1038/nrmicro885 15100690
4. Bakaletz LO Baker BD Jurcisek JA Harrison A Novotny LA Bookwalter JE Mungur R Munson RS Jr Demonstration of Type IV pilus expression and a twitching phenotype by Haemophilus influenzae Infect Immun 2005, 73(3):1635-1643. 1064948 15731063 10.1128/IAI.73.3.1635-1643.2005
5. Jurcisek JA Bakaletz LO Biofilms formed by nontypeable Haemophilus influenzae in vivo contain both double-stranded DNA and type IV pilin protein J Bacteriol 2007, 189(10):3868-3875. 1913342 17322318 10.1128/ JB.01935-06
6. Jurcisek JA Bookwalter JE Baker BD Fernandez S Novotny LA Munson RS Jr Bakaletz LO The PilA protein of non-typeable Haemophilus influenzae plays a role in biofilm formation, adherence to epithelial cells and colonization of the mammalian upper respiratory tract Mol Microbiol 2007, 65(5):1288-1299. 10.1111/j.1365-2958.2007.05864.x 17645732
7. Redfield RJ Cameron AD Qian Q Hinds J Ali TR Kroll JS Langford PR A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae J Mol Biol 2005, 347(4):735-747. 10.1016/ j.jmb.2005.01.012 15769466
8. VanWagoner TM Whitby PW Morton DJ Seale TW Stull TL Characterization of three new competence-regulated operons in Haemophilus influenzae J Bacteriol 2004, 186(19):6409-6421. 516621 15375121 10.1128/ JB.186.19.6409-6421.2004
9. Menard R Sansonetti PJ Parsot C Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells J Bacteriol 1993, 175(18):5899-5906. 206670 8376337
10. Mason KM Munson RS Jr Bakaletz LO A mutation in the sap operon attenuates survival of nontypeable Haemophilus influenzae in a chinchilla model of otitis media Infect Immun 2005, 73(1):599-608. 538956 15618200 10.1128/IAI.73.1.599-608.2005
11. Poje G Redfield RJ Transformation of Haemophilus influenzae Methods Mol Med 2003, 71:57-70. 12374031
12. Baba T Ara T Hasegawa M Takai Y Okumura Y Baba M Datsenko KA Tomita M Wanner BL Mori H Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: The Keio collection Mol Syst Biol 2006, 2:2006.0008. 16738554 16738554 1681482 10.1038/msb4100050
13. Datsenko KA Wanner BL One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Proc Natl Acad Sci USA 2000, 97(12):6640-6645. 18686 10829079 10.1073/pnas.120163297
14. Lee EC Yu D Martinez de Velasco J Tessarollo L Swing DA Court DL Jenkins NA Copeland NG A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA Genomics 2001, 73(1):56-65. 10.1006/geno.2000.6451 11352566
15. Copeland NG Jenkins NA Court DL Recombineering: A powerful new tool for mouse functional genomics Nat Rev Genet 2001, 2(10):769-779. 10.1038/ 35093556 11584293
16. Johnston DM Cannon JG Construction of mutant strains of Neisseria gonorrhoeae lacking new antibiotic resistance markers using a two gene cassette with positive and negative selection Gene 1999, 236(1):179-184. 10.1016/S0378-1119(99)00238-3 10433979
17. Campagnari AA Gupta MR Dudas KC Murphy TF Apicella MA Antigenic diversity of lipooligosaccharides of nontypable Haemophilus influenzae Infect Immun 1987, 55(4):882-887. 260433 3549563
18. Johnston JW Zaleski A Allen S Mootz JM Armbruster D Gibson BW Apicella MA Munson RS Jr Regulation of sialic acid transport and catabolism in Haemophilus influenzae Mol Microbiol 2007, 66(1):26-39. 10.1111/ j.1365-2958.2007.05890.x 17880422
19. Jurcisek J Greiner L Watanabe H Zaleski A Apicella MA Bakaletz LO Role of sialic acid and complex carbohydrate biosynthesis in biofilm formation by nontypeable Haemophilus influenzae in the chinchilla middle ear Infect Immun 2005, 73(6):3210-3218. 1111813 15908345 10.1128/ IAI.73.6.3210-3218.2005
20. Whitby PW Morton DJ Stull TL Construction of antibiotic resistance cassettes with multiple paired restriction sites for insertional mutagenesis of Haemophilus influenzae FEMS Microbiol Lett 1998, 158(1):57-60. 10.1111/j.1574-6968.1998.tb12800.x 9453156
21. Wang RF Kushner SR Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli Gene 1991, 100:195-199. 10.1016/0378-1119(91)90366-J 2055470
22. Briggs RE Tatum FM Generation and molecular characterization of new temperature-sensitive plasmids intended for genetic engineering of Pasteurellaceae Appl Environ Microbiol 2005, 71(11):7187-7195. 1287723 16269758 10.1128/AEM.71.11.7187-7195.2005
23. Dixon LG Albritton WL Willson PJ An analysis of the complete nucleotide sequence of the Haemophilus ducreyi broad-host-range plasmid pLS88 Plasmid 1994, 32(2):228-232. 10.1006/plas.1994.1060 7846148
24. Posfai G Koob MD Kirkpatrick HA Blattner FR Versatile insertion plasmids for targeted genome manipulations in bacteria: Isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome J Bacteriol 1997, 179(13):4426-4428. 179272 9209066
25. Hansen EJ Latimer JL Thomas SE Helminen M Albritton WL Radolf JD Use of electroporation to construct isogenic mutants of Haemophilus ducreyi J Bacteriol 1992, 174(16):5442-5449. 206384 1644771
26. Perez-Casal J Caparon MG Scott JR Mry, a trans-acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems J Bacteriol 1991, 173(8):2617-2624. 207828 1849511