β-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOG

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 59-64

  Gong, Haibiao ^a   Zhang, Bin ^b   Little, Garrick ^a   Kovar, Joy ^a   Chen, Huaxian ^a   Xie, Wen ^b   Schutz Geschwender, Amy ^a   Olive, D Michael ^a  

^a LI COR BIOSCIENCES   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

DDAOG; Fluorescence; Infrared; Reporter gene; Galactosidase

Indexed keywords

FLUORESCENCE; GENE EXPRESSION; INFRARED RADIATION; PLANTS (BOTANY); SUBSTRATES;

DDAOG; FLUORESCENT REPORTER; FLUORESCENT SIGNALS; GALACTOSIDASES; INDUCIBLE GENE-EXPRESSION SYSTEMS; REPORTER GENE; SIGNAL-TO-BACKGROUND RATIO; SUBSTRATE CONCENTRATIONS;

RED SHIFT;

7 HYDROXY 9H (I 3 DICHLORO 9,9 DIMETHYLACRIDIN 2 ONE); 9H (1,3 DICHLORO 9,9 DIMETHYLACRIDIN 2 ONE 7 YL) BETA DEXTRO GALACTOPYRANOSIDE; BETA GALACTOSIDASE; FLUORESCENT DYE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL STRAIN HEK293; COLORIMETRY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; INFRARED RADIATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION;

EID: 58349098972     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.11.031     Document Type: Article

References (22)

1. Serebriiskii I.G., and Golemis E.A. Uses of lacZ to study gene function: evaluation of β-galactosidase assays employed in the yeast two-hybrid system. Anal. Biochem. 285 (2000) 1-15
2. Rakhmanova V.A., and MacDonald R.C. A microplate fluorimetric assay for transfection of the β-galactosidase reporter gene. Anal. Biochem. 257 (1998) 234-237
3. Tang Y., Luo J., Fleming C.R., Kong Y., Olini Jr. G.C., Kong Y., Wildey M.J., Cavender D.E., and Demarest K.T. Development of a sensitive and HTS-compatible reporter gene assay for functional analysis of human adenosine A2a receptors in CHO-K1 cells. Assay Drug Dev. Technol. 2 (2004) 281-289
4. Fung P., Peng K., Kobel P., Dotimas H., Kauffman L., Olson K., and Eglen R.M. A homogeneous cell-based assay to measure nuclear translocation using β-galactosidase enzyme fragment complementation. Assay Drug Dev. Technol. 4 (2006) 263-272
5. Buller C.J., Zang X.P., Howard E.W., and Pento J.T. Measurement of β-galactosidase tissue levels in a tumor cell xenograft model. Methods Find. Exp. Clin. Pharmacol. 25 (2003) 713-716
6. Tung C.H., Zeng Q., Shah K., Kim D.E., Schellingerhout D., and Weissleder R. In vivo imaging of β-galactosidase activity using far red fluorescent switch. Cancer Res. 64 (2004) 1579-1583
7. Kalnins A., Otto K., Ruther U., and Muller-Hill B. Sequence of the lacZ gene of Escherichia coli. EMBO J. 2 (1983) 593-597
8. Appel S.H., Alpers D.H., and Tomkins G.M. Multiple molecular forms of β-galactosidase. J. Mol. Biol. 11 (1965) 12-22
9. Groth D., Keil O., Schneider M., and Reszka R. Transfection assay for dual determination of toxicity and gene expression. Anal. Biochem. 258 (1998) 141-143
10. Zhang Y.Z., Naleway J.J., Larison K.D., Huang Z.J., and Haugland R.P. Detecting lacZ gene expression in living cells with new lipophilic, fluorogenic β-galactosidase substrates. FASEB J. 5 (1991) 3108-3113
11. Martin C.S., Wight P.A., Dobretsova A., and Bronstein I. Dual luminescence-based reporter gene assay for luciferase and β-galactosidase. BioTechniques 21 (1996) 520-524
12. Zinselmeyer B.H., Beggbie N., Uchegbu I.F., and Schatzlein A.G. Quantification of β-galactosidase activity after non-viral transfection in vivo. J. Control. Release 91 (2003) 201-208
13. Lee W., Mitchell P., and Tjian R. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements. Cell 49 (1987) 741-752
14. Gong H., Singh S.V., Singh S.P., Mu Y., Lee J.H., Saini S.P., Toma D., Ren S., Kagan V.E., Day B.W., Zimniak P., and Xie W. Orphan nuclear receptor pregnane X receptor sensitizes oxidative stress responses in transgenic mice and cancerous cells. Mol. Endocrinol. 20 (2006) 279-290
15. Shyy J.Y., Lin M.C., Han J., Lu Y., Petrime M., and Chien S. The cis-acting phorbol ester "12-O-tetradecanoylphorbol 13-acetate"-responsive element is involved in shear stress-induced monocyte chemotactic protein 1 gene expression. Proc. Natl. Acad. Sci. USA 92 (1995) 8069-8073
16. Josserand V., Texier-Nogues I., Huber P., Favrot M.C., and Coll J.L. Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice. Gene Ther. 14 (2007) 1587-1593
17. Eustice D.C., Feldman P.A., Colberg-Poley A.M., Buckery R.M., and Neubauer R.H. A sensitive method for the detection of β-galactosidase in transfected mammalian cells. BioTechniques 11 (1991) 739-743
18. 2+ on the activity of β-galactosidase. Biochim. Biophys. Acta 250 (1971) 530-537
19. Jain V.K., and Magrath I.T. A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: application to quantitation of β-galactosidase in lacZ-transfected cells. Anal. Biochem. 199 (1991) 119-124
20. Weiss D.J., Liggitt D., and Clark J.G. Histochemical discrimination of endogenous mammalian β-galactosidase activity from that resulting from lac-Z gene expression. Histochem. J. 31 (1999) 231-236
21. Cecic I., Chan D.A., Sutphin P.D., Ray P., Gambhir S.S., Giaccia A.J., and Graves E.E. Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia. Mol. Imaging 6 (2007) 219-228
22. Kovar J.L., Simpson M.A., Schutz-Geschwender A., and Olive D.M. A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models. Anal. Biochem. 367 (2007) 1-12