Effect of porcine circovirus type 2 infection and replication on activated porcine peripheral blood mononuclear cells in vitro

Veterinary Immunology and Immunopathology

Volumn 127, Issue 3-4, 2009, Pages 350-356

  Yu, S ^a   Halbur, P G ^a   Thacker, E ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Apoptosis; PCV2; Peripheral blood mononuclear cells; Proliferation

Indexed keywords

CONCANAVALIN A; MESSENGER RNA; POKEWEED MITOGEN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL ACTIVATION; CELL PROLIFERATION; CELL STIMULATION; CELL VIABILITY; CIRCOVIRIDAE; COMPARATIVE STUDY; CONTROLLED STUDY; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; SWINE; VIRUS INFECTION; VIRUS REPLICATION;

ANIMALS; CELL PROLIFERATION; CELLS, CULTURED; CIRCOVIRUS; CONCANAVALIN A; GENE EXPRESSION REGULATION, VIRAL; LEUKOCYTES, MONONUCLEAR; POKEWEED MITOGENS; RNA, MESSENGER; SWINE; VIRAL PROTEINS; VIRUS REPLICATION;

MIRIDAE; PHYTOLACCACEAE; PORCINE CIRCOVIRUS 2; SUS;

EID: 58249120877     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2008.10.332     Document Type: Article

References (40)

1. Allan G.M., and Ellis J.A. Porcine circoviruses: a review. J. Vet. Diagn. Invest. 12 (2000) 3-14
2. Allan G.M., Mc Neilly F., Meehan B.M., Kennedy S., Mackie D.P., Ellis J.A., Clark E.G., Espuna E., Saubi N., Riera P., Botner A., and Charreyre C.E. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet. Microbiol. 66 (1999) 115-123
3. Allan G.M., McNeilly F., Kennedy S., Meehan B., Ellis J., and Krakowka S. Immunostimulation, PCV-2 and PMWS. Vet. Rec. 147 (2000) 170-171
4. Andree H.A., Reutelingsperger C.P., Hauptmann R., Hemker H.C., Hermens W.T., and Willems G.M. Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers. J. Biol. Chem. 265 (1990) 4923-4928
5. Bolin S.R., Stoffregen W.C., Nayar G.P., and Hamel A.L. Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived, colostrum-deprived piglets with type 2 porcine circovirus. J. Vet. Diagn. Invest. 13 (2001) 185-194
6. Chae C. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet. J. 168 (2004) 41-49
7. Cheung A.K., and Bolin S.R. Kinetics of porcine circovirus type 2 replication. Arch. Virol. 147 (2002) 43-58
8. Chianini F., Majo N., Segales J., Dominguez J., and Domingo M. Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet. Immunol. Immunopathol. 94 (2003) 63-75
9. Clark E. Post-weaning multisystemic wasting syndrome. Proceedings of the Western Canadaian Association of Swine Practitioners 28 (1997) 499-501
10. Darwich L., Segales J., Domingo M., and Mateu E. Changes in CD4(+), CD8(+), CD4(+)CD8(+), and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues. Clin. Diagn. Lab. Immunol. 9 (2002) 236-242
11. Dorn A.D., Waters W.R., Byers V.M., Pesch B.A., and Wannemuehler M.J. Characterization of mitogen-stimulated porcine lymphocytes using a stable fluorescent dye (PKH2) and multicolor flow cytometry. Vet. Immunol. Immunopathol. 87 (2002) 1-10
12. Fadok V.A., Voelker D.R., Campbell P.A., Cohen J.J., Bratton D.L., and Henson P.M. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol. 148 (1992) 2207-2216
13. Fenaux M., Halbur P.G., Haqshenas G., Royer R., Thomas P., Nawagitgul P., Gill M., Toth T.E., and Meng X.J. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J. Virol. 76 (2002) 541-551
14. Fulcher D., and Wong S. Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory. Immunol. Cell Biol. 77 (1999) 559-564
15. Grasland B., Loizel C., Blanchard P., Oger A., Nignol A.C., Bigarre L., Morvan H., Cariolet R., and Jestin A. Reproduction of PMWS in immunostimulated SPF piglets transfected with infectious cloned genomic DNA of type 2 porcine circovirus. Vet. Res. 36 (2005) 685-697
16. Krakowka S., Ellis J., McNeilly F., Meehan B., Oglesbee M., Alldinger S., and Allan G. Features of cell degeneration and death in hepatic failure and systemic lymphoid depletion characteristic of porcine circovirus-2-associated postweaning multisystemic wasting disease. Vet. Pathol. 41 (2004) 471-481
17. Krakowka S., Ellis J.A., McNeilly F., Ringler S., Rings D.M., and Allan G. Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2). Vet. Pathol. 38 (2001) 31-42
18. Kyriakis S.C., Saoulidis K., Lekkas S., Miliotis Ch.C., Papoutsis P.A., and Kennedy S. The effects of immuno-modulation on the clinical and pathological expression of postweaning multisystemic wasting syndrome. J. Comp. Pathol. 126 (2002) 38-46
19. Ladekjaer-Mikkelsen A.S., Nielsen J., Stadejek T., Storgaard T., Krakowka S., Ellis J., McNeilly F., Allan G., and Botner A. Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Vet. Microbiol. 89 (2002) 97-114
20. Lyons A.B., and Parish C.R. Determination of lymphocyte division by flow cytometry. J. Immunol. Methods 171 (1994) 131-137
21. Mandrioli L., Sarli G., Panarese S., Baldoni S., and Marcato P.S. Apoptosis and proliferative activity in lymph node reaction in postweaning multisystemic wasting syndrome (PMWS). Vet. Immunol. Immunopathol. 97 (2004) 25-37
22. Mannering S.I., Morris J.S., Jensen K.P., Purcell A.W., Honeyman M.C., van Endert P.M., and Harrison L.C. A sensitive method for detecting proliferation of rare autoantigen-specific human T cells. J. Immunol. Methods 283 (2003) 173-183
23. Nielsen J., Vincent I.E., Botner A., Ladekaer-Mikkelsen A.S., Allan G., Summerfield A., and McCullough K.C. Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Vet. Immunol. Immunopathol. 92 (2003) 97-111
24. Opriessnig T., Yu S., Gallup J.M., Evans R.B., Fenaux M., Pallares F., Thacker E.L., Brockus C.W., Ackermann M.R., Thomas P., Meng X.J., and Halbur P.G. Effect of vaccination with selective bacterins on conventional pigs infected with type 2 porcine circovirus. Vet. Pathol. 40 (2003) 521-529
25. Resendes A., Segales J., Balasch M., Calsamiglia M., Sibila M., Ellerbrok H., Mateu E., Plana-Duran J., Mankertz A., and Domingo M. Lack of an effect of a commercial vaccine adjuvant on the development of postweaning multisystemic wasting syndrome (PMWS) in porcine circovirus type 2 (PCV2) experimentally infected conventional pigs. Vet. Res. 35 (2004) 83-90
26. Resendes A.R., Majo N., Segales J., Espadamala J., Mateu E., Chianini F., Nofrarias M., and Domingo M. Apoptosis in normal lymphoid organs from healthy normal, conventional pigs at different ages detected by TUNEL and cleaved caspase-3 immunohistochemistry in paraffin-embedded tissues. Vet. Immunol. Immunopathol. 99 (2004) 203-213
27. Rosell C., Segales J., Plana-Duran J., Balasch M., Rodriguez-Arrioja G.M., Kennedy S., Allan G.M., McNeilly F., Latimer K.S., and Domingo M. Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J. Comp. Pathol. 120 (1999) 59-78
28. Sarli G., Mandrioli L., Laurenti M., Sidoli L., Cerati C., Rolla G., and Marcato P.S. Immunohistochemical characterisation of the lymph node reaction in pig post-weaning multisystemic wasting syndrome (PMWS). Vet. Immunol. Immunopathol. 83 (2001) 53-67
29. Schmid I., Krall W.J., Uittenbogaart C.H., Braun J., and Giorgi J.V. Dead cell discrimination with 7-amino-actinomycin d in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13 (1992) 204-208
30. Segales J., Alonso F., Rosell C., Pastor J., Chianini F., Campos E., Lopez-Fuertes L., Quintana J., Rodriguez-Arrioja G., Calsamiglia M., Pujols J., Dominguez J., and Domingo M. Changes in peripheral blood leukocyte populations in pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet. Immunol. Immunopathol. 81 (2001) 37-44
31. Segales J., and Domingo M. Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. Vet. Q. 24 (2002) 109-124
32. Segales J., Domingo M., Chianini F., Majo N., Dominguez J., Darwich L., and Mateu E. Immunosuppression in postweaning multisystemic wasting syndrome affected pigs. Vet. Microbiol. 98 (2004) 151-158
33. Segales J., Pastor J., Cuenca R., and Domingo M. Haematological parameters in postweaning multisystemic wasting syndrome-affected pigs. Vet. Rec. 146 (2000) 675-676
34. Shibahara T., Sato K., Ishikawa Y., and Kadota K. Porcine circovirus induces B lymphocyte depletion in pigs with wasting disease syndrome. J. Vet. Med. Sci. 62 (2000) 1125-1131
35. Takahashi R., Shimazaki C., Inaba T., Okano A., Hatsuse M., Okamoto A., Hirai H., Ashihara E., and Nakagawa M. A newly developed bisphosphonate, YM529, is a potent apoptosis inducer of human myeloma cells. Leuk. Res. 25 (2001) 77-83
36. Tuchscherer M., Kanitz E., Otten W., and Tuchscherer A. Effects of prenatal stress on cellular and humoral immune responses in neonatal pigs. Vet. Immunol. Immunopathol. 86 (2002) 195-203
37. Verfaillie T., Cox E., To L.T., Vanrompay D., Bouchaut H., Buys N., and Goddeeris B.M. Comparative analysis of porcine cytokine production by mRNA and protein detection. Vet. Immunol. Immunopathol. 81 (2001) 97-112
38. Waters W.R., Hontecillas R., Sacco R.E., Zuckermann F.A., Harkins K.R., Bassaganya-Riera J., and Wannemuehler M.J. Antigen-specific proliferation of porcine CD8alphaalpha cells to an extracellular bacterial pathogen. Immunology 101 (2000) 333-341
39. Yu S., Carpenter S., Opriessnig T., Halbur P.G., and Thacker E. Development of a reverse transcription-PCR assay to detect porcine circovirus type 2 transcription as a measure of replication. J. Virol. Methods 123 (2005) 109-112
40. Yu S., Vincent A., Opriessnig T., Carpenter S., Kitikoon P., Halbur P.G., and Thacker E. Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro. Vet. Microbiol. 123 (2007) 34-42