Apoptosis in normal lymphoid organs from healthy normal, conventional pigs at different ages detected by TUNEL and cleaved caspase-3 immunohistochemistry in paraffin-embedded tissues

Veterinary Immunology and Immunopathology

Volumn 99, Issue 3-4, 2004, Pages 203-213

  Resendes, A R ^a   Majo N ^a   Segales J ^a   Espadamala, J ^a   Mateu, E ^a   Chianini, F ^a   Nofrarias M ^a   Domingo, M ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Apoptosis; Cleaved caspase 3 immunohistochemistry; Lymphoid tissue; Swine; TUNEL

Indexed keywords

CASPASE 3; PARAFFIN;

AGE DISTRIBUTION; ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY LABELING; APOPTOSIS; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; EXPERIMENTAL MODEL; HISTOLOGY; IMMUNOHISTOCHEMISTRY; LYMPHOID ORGAN; MACROPHAGE; MESENTERY LYMPH NODE; NICK END LABELING; NONHUMAN; PEYER PATCH; SPLEEN; SWINE; THYMUS; TISSUE SECTION; TONSIL;

AGE FACTORS; ANIMALS; APOPTOSIS; CASPASE 3; CASPASES; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; IN SITU NICK-END LABELING; LYMPHOID TISSUE; SWINE;

SUS SCROFA;

EID: 2342568909     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2004.02.001     Document Type: Article

References (20)

1. Alam A., Cohen L.Y., Aouad S., Sekaly R.P. Early activation of caspases during T lymphocyte stimulation results in selective substrate cleavage in nonapoptotic cells. J. Exp. Med. 190:1999;1879-1890
2. Donoghue S., Baden H.S., Lauder I., Sobolewski S., Pringle J.H. Immunohistochemical localization of caspase-3 correlates with clinical outcome in B-cell diffuse large-cell lymphoma. Cancer Res. 59:1999;5386-5391
3. Dukers D.F., Oudejans J.J., Vos W., ten Berge R.L., Meijer C.J. Apoptosis in B-cell lymphomas and reactive lymphoid tissues always involves activation of caspase 3 as determined by a new in situ detection method. J. Pathol. 196:2002;307-315
4. Hengartner M.O. The biochemistry of apoptosis. Nature. 407:2000;770-776
5. Huppertz B., Frank H.G., Kaufmann P. The apoptosis cascade - morphological and immunohistochemical methods for its visualization. Anat. Embryol. 200:1999;1-18
6. Janeway, C.A., 2001. The development and survival of lymphocytes. In: Janeway, C.A., Travers, P., Walport, M., Shlomchik, M.J. (Eds.), Immunobiology: The Immune System in Health and Disease. Garland Publishing, New York, pp. 221-294.
7. Kelsoe G. The germinal center: a crucible for lymphocyte selection. Sem. Immunol. 8:1996;179-184
8. Kennedy N.J., Kataoka T., Tschopp J., Budd R.C. Caspase activation is required for T cell proliferation. J. Exp. Med. 190:1999;1891-1896
9. Kerr J.F., Wyllie A.H., Currie A.R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer. 26:1972;239-257
10. Labat-Moleur F., Guillermet C., Lorimier P., Robert C., Lantuejoul S., Brambilla E., Negoescu A. TUNEL apoptotic cell detection in tissue sections: critical evaluation and improvement critical evaluation and improvement. J. Histochem. Cytochem. 46:1998;327-334
11. Lenardo M., Chan K.M., Hornung F., McFarland H., Siegel R., Wang J., Zheng L. Mature T lymphocyte apoptosis-immune regulation in a dynamic and predictable antigenic environment. Annu. Rev. Immunol. 17:1999;221-253
12. Rathmell J.C., Thompson C.B. Pathways of apoptosis in lymphocyte development, homeostasis, and disease. Cell. 109:2002;97-107
13. Rodriguez-Ropon A., Hernandez-Jauregui P., Sanchez-Torres L., Favila-Castillo L., Estrada-Parra S., Moreno-Lopez J., Kennedy S. Apoptosis in lymph nodes and changes in lymphocyte subpopulations in peripheral blood of pigs infected with porcine rubulavirus. J. Comp. Pathol. 128:2003;1-8
14. Rosell C., Segales J., Plana-Duran J., Balasch M., Rodriguez-Arrioja G.M., Kennedy S., Allan G.M., McNeilly F., Latimer K.S., Domingo M. Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J. Comp. Pathol. 120:1999;59-78
15. Rosell C., Segales J., Domingo M. Hepatitis and staging of hepatic damage in pigs naturally infected with porcine circovirus type. Vet. Pathol. 37:2000;687-692
16. Sanchez-Cordon P.J., Romanini S., Salguero F.J., Nunez A., Bautista M.J., Jover A., Gomez-Villamos J.C. Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever. J. Comp. Pathol. 127:2002;239-248
17. Sato M., Mikami O., Kobayashi M., Nakajima Y. Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus. Vet. Microbiol. 75:2000;1-9
18. Stahelin B.J., Marti U., Solioz M., Zimmermann H., Reichen J. False positive staining in the TUNEL assay to detect apoptosis in liver and intestine is caused by endogenous nucleases and inhibited by diethyl pyrocarbonate. Mol. Pathol. 51:1998;204-208
19. Strasser A., Bouillet P. The control of apoptosis in lymphocyte selection. Immunol. Rev. 193:2003;82-92
20. Zhivotovsky B., Samali A., Gahm A., Orrenius S. Caspases: their intracellular localization and translocation during apoptosis. Cell Death Differ. 6:1999;644-651