Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory

Immunology and Cell Biology

Volumn 77, Issue 6, 1999, Pages 559-564

  Fulcher, D A ^a   Wong, S W J ^a  

^a WESTMEAD HOSPITAL   (Australia)

Author keywords

3H thymidine; Blastogenesis; Carboxyfluorescein succinimidyl ester; CD25; CD69; T lymphocytes

Indexed keywords

ANTIGEN; CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; CD69 ANTIGEN; FLUORESCENT DYE; INTERLEUKIN 2 RECEPTOR; MITOGENIC AGENT; PHYTOHEMAGGLUTININ; THYMIDINE; UNCLASSIFIED DRUG;

ANTIGEN SPECIFICITY; ARTICLE; CANDIDA; CELL FUNCTION; CELL LABELING; CONTROLLED STUDY; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; LYMPHOCYTE TRANSFORMATION; MITOSIS; STAINING; SYSTEMIC LUPUS ERYTHEMATOSUS; T LYMPHOCYTE;

ANTIGENS, CD; ANTIGENS, FUNGAL; CANDIDA; CELL DIVISION; EPITOPES; EVALUATION STUDIES; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMANS; IMMUNOLOGIC DEFICIENCY SYNDROMES; INFANT; LYMPHOCYTE ACTIVATION; PHYTOHEMAGGLUTININS; PREDICTIVE VALUE OF TESTS; REPRODUCIBILITY OF RESULTS; SUCCINIMIDES; T-LYMPHOCYTES; THYMIDINE;

CANDIDA;

EID: 0032733633     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1711.1999.00870.x     Document Type: Article

References (5)

1. 1 Report of a WHO Scientific Group. Primary immunodeficiency diseases. Clin. Exp. Immunol. 1997; 109 (Suppl. 1): S1-28.
2. 2 Maluish AE, Strong DM. Lymphocyte proliferation. In: Rose NR, Friedman HF, Fahey JL (eds). Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology, 1986; 274-81.
3. 3 Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J. Immunol. Meth. 1994; 171: 131-7.
4. 4 Angulo R, Fulcher DA. Measurement of Candida-specific blastogenesis - Comparison of carboxyfluorescein succinimidyl ester-labelling of T-cells, thymidine incorporation and CD69 expression. Comm. Clin. Cytometry 1998; 34: 143-51.
5. 5 Biselli R, Matricardi PM, D'Amelio R, Fattorossi A. Multiparametric flow cytometric analysis of the kinetics of surface molecule expression after polyclonal activation of human peripheral blood T lymphocytes. Scand. J. Immunol. 1992; 35: 439-47.