Identification of small molecule inhibitors of the mitotic kinase haspin by high-throughput screening using a homogeneous time-resolved fluorescence resonance energy transfer assay

Journal of Biomolecular Screening

Volumn 13, Issue 10, 2008, Pages 1025-1034

  Patnaik, Debasis ^a   Xian, Jun ^a   Glicksman, Marcie A ^a   Cuny, Gregory D ^a   Stein, Ross L ^a   Higgins, Jonathan M G ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Antimitotics; Chromatin; Haspin; Kinase; Time resolved fluorescence resonance energy transfer

Indexed keywords

ADENOSINE TRIPHOSPHATE; EUROPIUM; HASPIN; HISTONE H3; MONOCLONAL ANTIBODY; PHOSPHOTRANSFERASE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); ENZYME ASSAY; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC 50; MEDICAL RESEARCH; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RADIOMETRY;

AMINO ACID SEQUENCE; BIOLOGICAL ASSAY; ENZYME STABILITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; INHIBITORY CONCENTRATION 50; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN KINASE INHIBITORS; PROTEIN-SERINE-THREONINE KINASES; RADIOMETRY; STAUROSPORINE; SUBSTRATE SPECIFICITY; TIME FACTORS;

EID: 58149387464     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057108326081     Document Type: Article

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