The effect of inhibited replication on DNA migration in the comet assay in relation to cytotoxicity and clastogenicity

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 695, Issue 1-2, 2008, Pages 22-27

  Speit, Günter ^a   Schütz, Petra ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Aphidicolin; Comet assay; Genotoxicity; Hydroxyurea; Replication

Indexed keywords

APHIDICOLIN; CLASTOGEN; DNA; HYDROXYUREA; CYTOTOXIN; MUTAGENIC AGENT;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL STRAIN V 79; CHROMOSOME ABERRATION; COMET ASSAY; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; DNA REPLICATION; DNA SYNTHESIS INHIBITION; IN VITRO STUDY; LONG TERM EXPOSURE; MITOSIS INDEX; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; ANIMAL; CELL LINE; CHEMICALLY INDUCED DISORDER; CRICETULUS; DRUG EFFECT; HAMSTER; METHODOLOGY;

ANIMALS; APHIDICOLIN; CELL CYCLE; CELL LINE; CHROMOSOME ABERRATIONS; COMET ASSAY; CRICETINAE; CRICETULUS; CYTOTOXINS; DNA; DNA REPLICATION; HYDROXYUREA; MUTAGENS;

EID: 58149311023     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2008.06.009     Document Type: Article

References (16)

1. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 2000, 35:206-221.
2. Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B., Clay P., Collins A., Smith A., Speit G., Thybaud V., Tice R.R. Recommendations for conducting the in vivo alkaline comet assay. Mutagenesis 2003, 18:45-51.
3. Burlinson B., Tice R.R., Speit G., Agurell E., Brendler-Schwaab S.Y., Collins A.R., Escobar P., Honma M., Kumaravel T.S., Nakajima M., Sasaki Y.F., Thybaud V., Uno Y., Vasquez M., Hartmann A. Proceedings of the Fourth International Workgroup on Genotoxicity Testing: Results of the In Vivo Comet Assay Workgroup. Mutat. Res. 2007, 627:31-35.
4. Speit G., Hartmann A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair. Methods Mol. Biol. 2006, 314:275-286.
5. Henderson L., Wolfreys A., Fedyk J., Bourner C., Windebank S. The ability of the comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 1998, 13:89-94.
6. Hartmann A., Kiskinis E., Fjallman A., Suter W. Influence of cytotoxicity and compound precipitation on test results in the alkaline comet assay. Mutat. Res. 2001, 497:199-212.
7. Hartmann A., Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol. Lett. 1997, 90:183-188.
8. Gedik C.M., Ewen S.W., Collins A.R. Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol. 1992, 62:313-320.
9. Speit G., Hartmann A. The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay). Mutagenesis 1995, 10:555-559.
10. Martin F.L., Cole K.J., Orme M.H., Grover P.L., Phillips D.H., Venitt S. The DNA repair inhibitors hydroxyurea and cytosine arabinoside enhance the sensitivity of the alkaline single-cell gel electrophoresis ('comet') assay in metabolically-competent MCL-5 cells. Mutat. Res. 1999, 445:21-43.
11. Speit G., Witton-Davies T., Heepchantree W., Trenz K., Hoffmann H. Investigations on the effect of cigarette smoking in the comet assay. Mutat. Res. 2003, 542:33-42.
12. Speit G., Schutz P., Hoffmann H. Enhancement of genotoxic effects in the comet assay with human blood samples by aphidicolin. Toxicol. Lett. 2004, 153:303-310.
13. Guerci A., Liviac D., Marcos R. Detection of excision repaired DNA damage in the comet assay by using ara-C and hydroxyurea in three different cell types. Cell Biol. Toxicol. 2007.
14. Cipollini M., He J., Rossi P., Baronti F., Micheli A., Rossi A.M., Barale R. Can individual repair kinetics of UVC-induced DNA damage in human lymphocytes be assessed through the comet assay?. Mutat. Res. 2006, 601:150-161.
15. Greenwood S.K., Hill R.B., Sun J.T., Armstrong M.J., Johnson T.E., Gara J.P., Galloway S.M. Population doubling: a simple and more accurate estimation of cell growth suppression in the in vitro assay for chromosomal aberrations that reduces irrelevant positive results. Environ. Mol. Mutagen. 2004, 43:36-44.
16. Speit G., Haupter S., Hartmann A. Evaluation of the genotoxic properties of paraquat in V79 Chinese hamster cells. Mutat. Res. 1998, 412:187-193.