Alcalase®-catalysed synthesis of the precursor tetrapeptide N-benzoylarginylglycylaspartylserinamide (Bz-RGDS-NH2) of the cell-adhesion peptide arginylglycylaspartylserine (RGDS)

Biotechnology and Applied Biochemistry

Volumn 51, Issue 3, 2008, Pages 119-127

  Huang, Yi Bing ^a   Yang, Sen ^a   Cai, Yu ^a   Wang, Hua ^a,b   Gao, Gui ^a   Wu, Xiao Xia ^a   Xu, Li ^a   Zhang, Xue Zhong ^a  

^a JILIN UNIVERSITY   (China)

^b CHINA JAPAN UNION HOSPITAL OF JILIN UNIVERSITY   (China)

Author keywords

Alcalase catalysed peptide synthesis; Arginylglycylaspartylserine (RGDS); Cell adhesion peptide; N benzoylarginylglycylaspartylserinamide (Bz RGDS NH2); Organic solvents

Indexed keywords

ALCALASE; ALKALINE PROTEASE; ARGINYLGLYCYLASPARTYLSERINE (RGDS); ASPARTIC ACIDS; BUFFER SYSTEM; CHEMICAL METHOD; ENZYMATIC METHODS; ETHYL ESTERS; GRAM SCALE; KINETIC CONTROL; L-ARGININE; MOLAR RATIO; N-BENZOYLARGINYLGLYCYLASPARTYLSERINAMIDE (BZ-RGDS-NH2); NOVEL ROUTE; OPTIMUM REACTION CONDITIONS; ORGANIC MEDIA; PEPTIDE PRODUCTS; PH VALUE; REACTION TEMPERATURE; SUBSTRATE SPECIFICITY; TETRAPEPTIDE; TRIPEPTIDE; WATER-MISCIBLE ORGANIC SOLVENTS;

ADHESION; AMINO ACIDS; CATALYSIS; CELL MEMBRANES; ESTERS; ORGANIC ACIDS; ORGANIC SOLVENTS; PH EFFECTS; SODIUM; SUBSTRATES; SYNTHESIS (CHEMICAL); WATER CONTENT;

AMINES;

ARGINYLGLYCYLASPARTYLSERINE; ASPARTIC ACID; BUFFER; N BENZOYLARGINYLGLYCYLASPARTYLSERINAMIDE; SERINE; SUBTILISIN; TETRAPEPTIDE; UNCLASSIFIED DRUG; ARGINYL GLYCYL ASPARTYL SERINE; ARGINYL-GLYCYL-ASPARTYL-SERINE; N-BENZOYLARGINYLGLYCYLASPARTYLSERINAMIDE; OLIGOPEPTIDE; SOLVENT; WATER;

ACETYLATION; ARTICLE; CATALYSIS; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; PEPTIDE SYNTHESIS; PH; PROCESS OPTIMIZATION; PROTEIN HYDROLYSIS; REACTION TIME; SOLVENT EFFECT; TEMPERATURE; WATER CONTENT; CELL ADHESION; CHEMISTRY; IMMUNOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; SOLUBILITY; SYNTHESIS; TIME;

CATALYSIS; CELL ADHESION; HYDROGEN-ION CONCENTRATION; KINETICS; OLIGOPEPTIDES; SOLUBILITY; SOLVENTS; SUBSTRATE SPECIFICITY; SUBTILISINS; TEMPERATURE; TIME FACTORS; WATER;

EID: 58149288663     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070123     Document Type: Article

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