Application of membrane phase separation for enzymatic synthesis of aspartame precursor in biphasic reaction system

Biochemical Engineering Journal

Volumn 4, Issue 2, 2000, Pages 143-147

  Isono, Yasuyuki ^a,b   Nakajima, Mitsutoshi ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b Dainichiseika Color & Chemicals Mfg Co Ltd   (Japan)

Author keywords

Aspartame precursor; Biphasic reaction; Enzyme reactor; Hydrophobic membrane; Phase separation

Indexed keywords

ASPARTAME; HEXANOL; PHENYLALANINE METHYL ESTER;

ARTICLE; ENZYME ACTIVITY; FERMENTATION; HYDROPHOBICITY; MEMBRANE REACTOR; PRIORITY JOURNAL; SYNTHESIS;

EID: 0033990930     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-703X(99)00037-6     Document Type: Article

References (17)

1. Isowa Y., Ohmori M., Ichikawa T., Mori K., Nonaka Y., Kihara K., Oyama K., Satoh H., Nishimura S. The thermolysin-catalyzed condensation reaction of N-substituted aspartic and glutamic acids with phenylalanine methyl ester. Tetrahedron Lett. 28:1979;2611-2622.
2. Murakami Y., Hirata A. Enzymatic synthesis of peptide - review. Seibutsu Kogaku. 76:1998;238-254.
3. Nakanishi K., Kimura Y., Matsuno R. Kinetic and equilibrium of enzymatic synthesis of peptide in aqueous/organic biphasic system. Eur. J. Biochem. 161:1986;541-549.
4. Hirata M., Ishimine T., Hirata A. Development of novel method for enzymatic peptide synthesis utilizing extractive reactor. Kagaku Kogaku Ronbunshu. 30:1997;467-477.
5. Y. Okahata, K. Ijiro, Functional capsule membrane, part 29. Thermolysin-immobilized capsule membrane as bioreactors in the synthesis of a dipeptide (precursor of aspartame) in an organic solvent, J. Chem. Soc. Perkin Trans. 2 (1988) 91-96.
6. Nagayasu T., Miyanaga M., Tanaka T., Sakiyama T., Nakanishi K. Synthesis of aspartame precursor with an immobilized thermolysin in tert-amyl alcohol. Biotechnol. Bioeng. 43:1994;1118-1123.
7. K. Ridgway, E.E. Thorpe, in: T.C. Lo, M.H. Baird, C. Hanson (Eds.), Handbook of solvent extraction, Wiley, New York, 1983, Chzp. 19.
8. Komasawa I., Kuromi M., Tone S. Extraction of weakly basic compounds with physiological activity. Kagaku Kogaku Ronbunshu. 13:1987;223-229.
9. Hano T., Matsumoto M., Kawazu T., Ohtake T. Separation of di- and tripeptides with solvent extraction and an emulsion liquid membrane. J. Chem. Tech. Biotechnol. 62:1995;60-63.
10. C.J. King, Separation Processes, McGraw-Hill, New York, 1971, pp. 740-746.
11. Goto M., Kondo K., Nakashio F. Protein extraction by reversed micelles using dioleyl phosphoric acid. J. Chem. Eng. Jpn. 23:1990;513-515.
12. Kuboi R., Hashimoto K., Komasawa I. Separation of proteins with reverse micellar liquid membranes. Kagaku Kogaku Ronbunshu. 16:1990;335-342.
13. Isono Y., Nabetani H., Nakajima M. Hollow-fiber enzyme reactor integrated with solvent extraction for synthesis of aspartame precursor. Process Biochem. 30:1995;773-776.
14. Hlavacek M. Break-up of oil-water emulsion induced by permeation through a microfiltration membrane. J. Membr. Sci. 102:1995;1-7.
15. Ueyama K., Kittaka S., Furusaki S. Demulsification of using porous teflon membrane. Kagaku Kogaku Ronbunshu. 10:1984;775-778.
16. Ueyama K., Fukuura K., Furusaki S. Oil-phase permeation behavior of O/W emulsion through a porous polytetrafluoroethylene membrane. J. Chem. Eng. Jpn. 20:1987;618-622.
17. Pronk W., van der Burgt M., Boswinkel G., van't Riet K. A hybrid membrane-emulsion reactor for the enzymatic hydrolysis of lipids. J. Amer. Oil Chem. Soc. 68:1991;852-856.