Positional-scanning fluorigenic substrate libraries reveal unexpected specificity determinants of DUBs (deubiquitinating enzymes)

Biochemical Journal

Volumn 415, Issue 3, 2008, Pages 367-375

  Drag, Marcin ^a,b   Mikolajczyk, Jowita ^a   Bekes, Miklos ^a,c   Reyes Turcu, Francisca E ^d   Ellman, Jonathan A ^e   Wilkinson, Keith D ^d   Salvesen, Guy S ^a  

^a BURNHAM INSTITUTE   (United States)

^b WROCLAW UNIVERSITY OF TECHNOLOGY   (Poland)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Combinatorial chemistry; Deubiquitinating enzyme (DUB); Fluorigenic substrate; Positional scanning substrate combinatorial library (PS SCL); Protease; Ubiquitin

Indexed keywords

COMBINATORIAL CHEMISTRY; DEUBIQUITINATING ENZYME (DUB); FLUORIGENIC SUBSTRATE; POSITIONAL-SCANNING SUBSTRATE COMBINATORIAL LIBRARY (PS-SCL); PROTEASE; UBIQUITIN;

AMINES; AMINO ACIDS; BIOASSAY; BIOCHIPS; CATALYSTS; ENZYME ACTIVITY; ENZYMES; LIBRARIES; SCANNING; TUMORS;

SUBSTRATES;

ARGININE; CITRATE SODIUM; CYSTEINE PROTEINASE; DEUBIQUITINATING ENZYME; FLOURIGENIC TETRAPEPTIDE; GLYCINE; ISOPEPTIDASE T; OVARIAN TUMOR 1; PAPAIN LIKE PROTEASE; PROTEINASE; TETRAPEPTIDE; UBIQUITIN; UBIQUITIN C TERMINAL HYDROLASE L3; UNCLASSIFIED DRUG; FLUORESCENT DYE; PEPTIDE LIBRARY; UBIQUITIN ISOPEPTIDASE; UBIQUITIN SPECIFIC PROTEASE; UBIQUITIN THIOLESTERASE; UBIQUITIN-SPECIFIC PROTEASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HUMAN; POSITIONAL SCANNING SUBSTRATE COMBINATORIAL LIBRARY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; SEQUENCE ANALYSIS; COMBINATORIAL CHEMISTRY; KINETICS; MASS SPECTROMETRY; METABOLISM; PROTEIN PROCESSING;

CATALYSIS; CATALYTIC DOMAIN; COMBINATORIAL CHEMISTRY TECHNIQUES; ENDOPEPTIDASES; FLUORESCENT DYES; HUMANS; KINETICS; MASS SPECTROMETRY; PEPTIDE LIBRARY; PROTEIN PROCESSING, POST-TRANSLATIONAL; SUBSTRATE SPECIFICITY; UBIQUITIN; UBIQUITIN THIOLESTERASE;

EID: 58149159450     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20080779     Document Type: Article

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