Progressive rearrangement of subtilisin Carlsberg into orderly and inflexible conformation with Ca2+ binding

Biophysical Journal

Volumn 81, Issue 5, 2001, Pages 2972-2978

  Lee, S ^a   Jang, D J ^a  

^a Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; SUBTILISIN; TYROSINE;

ANISOTROPY; ARTICLE; BINDING SITE; CALCIUM BINDING; CIRCULAR DICHROISM; DEPOLARIZATION; DIFFERENTIAL SCANNING CALORIMETRY; DISSOCIATION; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME STABILITY; ENZYME STRUCTURE; FLUORESCENCE; KINETICS;

EID: 0034764538     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75937-1     Document Type: Article

References (38)

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2. The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis
3. Flexibility of enzymes suspended in organic solvents probed by time-resolved fluorescence anisotropy: Evidence that enzyme activity and enantioselectivity are directly related to enzyme flexibility
4. Dissecting the catalytic triad of a serine protease
5. Circular dichroic analysis of protein conformation: Inclusion of β-turns
6. Kinetic analysis of the mechanism for subtilisin in essentially anhydrous organic solvents
7. Control of subtilisin substrate specificity by solvent engineering in organic solvents and supercritical fluoroform
8. Metallopeptide design: Tuning the metal cation affinities with unnatural amino acids and peptide secondary structure
9. X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile
10. Stability of subtilisins and related proteinases (subtilases)
11. Secondary structure nucleation in peptides: Transition metal ion stabilized α-helices
12. A convergent approach to protein design: Metal ion-assisted spontaneous self-assembly of a polypeptide into a triple-helix bundle protein
13. The fluorescence decay of tryptophan residues in native and denatured proteins
14. Metal ion-dependent modulation of the dynamics of a designed protein
15. A weak calcium binding site in subtilisin BPN' has a dramatic effect on protein stability
16. Fluorescence spectroscopic study of subtilisins as relevant to their catalytic activity in aqueous-organic media
17. Metal ion induced folding of a de novo designed coiled-coil peptide
18. Rotational freedom of tryptophan residues in proteins and peptides
19. Solvent-exposed tryptophans probe the dynamics at protein surfaces
20. Cation-binding sites of subtilisin Carlsberg probed with Eu(III) luminescence
21. Iron(II) organizes a synthetic peptide into three-helix bundles
22. Structural comparison of two serine proteinase-protein inhibitor complexes: Eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo
23. The refined crystal structure of subtilisin Carlsberg at 2.5 Å resolution
24. Internal motion of lysozyme studied by time-resolved fluorescence depolarization of tryptophan residues
25. Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding
26. The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: Subtilisin as a test case
27. Metal ion enhanced helicity in synthetic peptides containing unnatural, metal-ligating residues
28. A new basis for interpreting the circular dichroism spectra of proteins
29. Synthesis and efficacy of square planar copper complexes designed to nucleate β-sheet structure
30. Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
31. Subtilisin Carlsberg: V. The complete sequence; comparison with subtilisin BPN': Evolutionary relationships
32. An engineered disulfide cross-link accelerates the refolding rate of calcium-free subtilisin by 850-fold
33. Metal coordination as a method for templating peptide conformation
34. Determination of protein secondary structure
35. Role of bound calcium ions in thermostable, proteolytic enzymes: Separation of intrinsic and calcium ion contributions to the kinetic thermal stability
36. Resolution of tyrosyl and tryptophyl fluorescence emission from subtilisins
37. Polyethylene glycol-induced stabilization of subtilisin