Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastoris

Protein Expression and Purification

Volumn 64, Issue 1, 2009, Pages 47-54

  Egawa, Kyoko ^a   Shibata, Hiroyuki ^b   Yamashita, Shun ichi ^a   Yurimoto, Hiroya ^a   Sakai, Yasuyoshi ^a,c   Kato, Hiroaki ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

^c CREST ^*

Author keywords

Fed batch fermentation; Heterologous expression; Membrane protein; Peroxisome; Pichia pastoris; PMP22

Indexed keywords

MEMBRANE PROTEIN; PXMP2 PROTEIN, RAT; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOREACTOR; CELL NUCLEUS MEMBRANE; CHEMISTRY; FERMENTATION; FUNGAL GENE; GENETICS; INTRACELLULAR MEMBRANE; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MICROBODY; PEROXISOME; PICHIA; PLASMID; RAT; SOLUBILITY;

ANIMALS; BIOREACTORS; FERMENTATION; GENES, FUNGAL; INTRACELLULAR MEMBRANES; MEMBRANE PROTEINS; MICROBODIES; NUCLEAR ENVELOPE; PEROXISOMES; PICHIA; PLASMIDS; RATS; RECOMBINANT PROTEINS; SOLUBILITY;

PICHIA PASTORIS; RATTUS;

EID: 57649093478     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.10.004     Document Type: Article

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