A recombinant MnSOD is radioprotective for normal cells and radiosensitizing for tumor cells

Free Radical Biology and Medicine

Volumn 46, Issue 1, 2009, Pages 110-116

  Borrelli, Antonella ^a   Schiattarella, Antonella ^a   Mancini, Roberto ^b   Morrica, Brunello ^c   Cerciello, Vincenzo ^c   Mormile, Maria ^c   d'Alesio, Valentina ^d   Bottalico, Laura ^d   Morelli, Francesco ^b   D'Armiento, Maria ^e   D'Armiento, Francesco Paolo ^e   Mancini, Aldo ^a  

^a NATIONAL CANCER INSTITUTE   (Italy)

^b INSTITUTE OF GENETICS AND BIOPHYSICS ADRIANO BUZZATI TRAVERSO   (Italy)

^c NATIONAL CANCER INSTITUTE   (Italy)

^d Malzoni Radiosurgery Center   (Italy)

^e UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Cell population; Radioprotection; Radiosensitizing; Viability

Indexed keywords

MANGANESE SUPEROXIDE DISMUTASE; RECOMBINANT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL STRAIN; CONTROLLED STUDY; FEMALE; IN VITRO STUDY; IN VIVO STUDY; IONIZING RADIATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIATION INJURY; RADIATION PROTECTION; RADIOSENSITIZATION; TUMOR CELL;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL LINE, TUMOR; FEMALE; FIBROBLASTS; FREE RADICAL SCAVENGERS; FREE RADICALS; GENE EXPRESSION; HUMANS; LETHAL DOSE 50; MEMBRANE PROTEINS; MICE; MICE, INBRED C57BL; NEOPLASMS; RADIATION INJURIES; RADIATION TOLERANCE; RADIATION, IONIZING; RADIATION-SENSITIZING AGENTS; RECOMBINANT PROTEINS; SUPEROXIDE DISMUTASE;

ANIMALIA; MURINAE;

EID: 57649093466     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2008.10.030     Document Type: Article

References (32)

1. Epperly M.W., Gretton J.E., Sikora C.A., Jefferson M., Bernarding M., Nie S., and Greenberger J.S. Mitochondrial localization of superoxide dismutase is required for decreasing radiation cellular damage. Radiat. Res. 160 (2003) 568-578
2. Valko M., Rhodes C.J., Moncol J., Izakovic M., and Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem.-Biol. Interact. 160 (2006) 1-40
3. Robbins M.E., and Zhao W. Chronic oxidative stress and radiation-induced late normal tissue injury: a review. Int. J. Radiat. Biol. 80 (2004) 251-259
4. Zhao W., Diz D.I., and Robbins M.E. Oxidative damage pathways in relation to normal tissue injury. Br. J. Radiol. 80 (2007) 23-31
5. Kalen A.L., Sarsour E.H., Venkataraman S., and Goswami P.C. Mn-superoxide dismutase overexpression enhances G2 accumulation and radioresistance in human oral squamous carcinoma cells. Antioxid. Redox Signal. 8 (2006) 1273-1281
6. Martin R.C., Ahn J., Nowell S.A., Hein D.W., Doll M.A., Martini B.D., et al. Association between manganese superoxide dismutase promoter gene polymorphism and breast cancer survival. Breast Cancer Res. 8 (2006) R45
7. Stenlund P., and Tibell L.A. Chimeras of human extracellular and intracellular superoxide dismutases. Analysis of structure and function of the individual domains. Protein Eng. 12 (1999) 319-325
8. Greenberger J.S., Epperly M.W., Gretton J., Jefferson M., Nie S., Bernarding M., Kagan V., and Guo H.L. Radioprotective gene therapy. Curr. Gene Ther. 3 (2003) 183-195
9. Mancini A., Borrelli A., Schiattarella A., Fasano S., Occhiello A., Pica A., Tommasino M., Nüesch J.P.F., and Rommelaere J. Tumor suppressive activity of a variant isoform of Mn superoxide dismutase released by a human liposarcoma cell line. Int. J. Cancer 119 (2006) 932-943
10. Mancini A., Borrelli A., Schiattarella A., Aloj L., Aurilio M., Morelli F., Pica A., Occhiello A., Lorizio R., Mancini R., Sica A., Mazzarella L., Sica F., Grieco P., Novellino E., Pagnozzi D., Pucci P., and Rommelaere J. Biophysical and biochemical characterization of a liposarcoma derived recombinant MnSOD protein acting as anticancer agent. Int. J. Cancer 123 (2008) 2684-2695
11. McCord J.M., and Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244 (1969) 6049-6055
12. Mancini A., Garbi C., D'Armiento F., Borrelli A., and Ambesi-Impiombato F.S. Culture and cloning of an adipocytes cell line from a human liposarcoma. Boll. Ist. Tumori Napoli 38 (1991) 43-49
13. Franken N.A., Rodermond H.M., Stap J., Haveman J., and van Bree C. Clonogenic assay of cells in vitro. Nat. Protoc. 1 (2006) 2315-2319
14. Biade S., Stobbe C., and Chapman J.D. The intrinsic radiosensitivity of some human tumor cells throughout their cell cycles. Radiat. Res. 147 (1997) 416-421
15. Tucker S.L. Is the mean inactivation dose a good measure of cell radiosensitivity?. Radiat. Res. 105 (1986) 18-26
16. Tucker S.L. Parameters of radiosensitivity. Radiat. Res. 108 (1986) 226-229
17. Fertil B., and Malaise E.P. Intrinsic radiosensitivity of human cell lines is correlated with radioresponsiveness of human tumors: analysis of 101 published survival curves. Int. J. Radiat. Oncol. Biol. Phys. 11 (1985) 1699-1707
18. Malaise E.P., Fertil B., Chavaudra N., and Guichard M. Distribution of radiation sensitivities for human tumor cells of specific histological types: comparison of in vitro to in vivo data. Int. J. Radiat. Oncol. Biol. Phys. 12 (1986) 617-624
19. Deschavanne P.J., Debieu D., Fertil B., and Malaise E.P. Re-evaluation of in vitro radiosensitivity of human fibroblasts of different genetic origins. Int. J. Radiat. Biol. 50 (1986) 279-293
20. Malaise E.P., Fertil B., Deschavanne P.J., Chavaudra N., and Brooks W.A. Initial slope of radiation survival curves is characteristic of the origin of primary and established cultures of human tumor cells and fibroblasts. Radiat. Res. 111 (1987) 319-333
21. Chomczynski P., and Sacchi N. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat. Protoc. 1 (2006) 581-585
22. Chung-man Ho J., Zheng S., Comhair S.A., Farver C., and Erzurum S.C. Differential expression of manganese superoxide dismutase and catalase in lung cancer. Cancer Res. 61 (2001) 8578-8585
23. Pljesa-Ercegovac M., Mimic-Oka J., Dragicevic D., Savic-Radojevic A., Opacic M., Pljesa S., Radosavljevic R., and Simic T. Altered antioxidant capacity in human renal cell carcinoma: role of glutathione associated enzymes. Urol. Oncol. 26 (2008) 175-1781
24. Greenberger J.S., and Epperly M.W. Antioxidant gene therapeutic approaches to normal tissue radioprotection and tumor radiosensitization. In Vivo 21 (2007) 141-146
25. Epperly M.W., Epstein C.J., Travis E.L., and Greenberger J.S. Decreased pulmonary radiation resistance of manganese superoxide dismutase (MnSOD)-deficient mice is corrected by human manganese superoxide dismutase-plasmid/liposome (SOD2-PL) intratracheal gene therapy. Radiat. Res. 154 (2000) 365-374
26. Dolgachev V., Oberley L.W., Huang T.T., Kraniak J.M., Tainsky M.A., Hanada K., and Separovic D. A role for manganese superoxide dismutase in apoptosis after photosensitization. Biochem. Biophys. Res. Commun. 332 (2005) 411-417
27. Murley J.S., Kataoka Y., Cao D., Li J.J., Oberley L.W., and Grdina D.J. Delayed radioprotection by NF kappa B-mediated induction of Sod2 (MnSOD) in SA-NH tumor cells after exposure to clinically used thiol-containing drugs. Radiat. Res. 162 (2004) 536-546
28. Yan S., Brown S.L., Kolozsvary A., Freytag S.O., Lu M., and Kim J.H. Mitigation of radiation-induced skin injury by AAV2-mediated MnSOD gene therapy. J. Gene Med. 10 (2008) 1012-1018
29. Chau B.N., Cheng E.H., Kerr D.A., and Hardwick J.M. Aven, a novel inhibitor of caspase activation, binds Bcl-xL and Apaf-1. Mol. Cell 6 (2000) 31-40
30. Liu X., Kim C.N., Yang J., Jemmerson R., and Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86 (1996) 147-157
31. Bertini I., Cavallaro G., and Rosato A. Cytochrome c: occurrence and functions. Chem. Rev. 106 (2006) 90-115
32. Bernard D., Gosselin K., Monte D., Vercamer C., Bouali F., Pourtier A., Vandenbunder B., and Abbadie C. Involvement of Rel/nuclear factor-β transcriptor factors in keratinocytes senescence. Cancer Res. 64 (2004) 472-481