Stable transfection of Eimeria tenella: Constitutive expression of the YFP-YFP molecule throughout the life cycle

International Journal for Parasitology

Volumn 39, Issue 1, 2009, Pages 109-117

  Yan, Wenchao ^a   Liu, Xianyong ^a   Shi, Tuanyuan ^a   Hao, Lili ^a   Tomley, Fiona M ^b   Suo, Xun ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Eimeria tenella; Immunogenicity; Reproduction; Stable transfection

Indexed keywords

DNA; YELLOW FLUORESCENT PROTEIN;

ANTIGEN; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; LIFE CYCLE; POULTRY; PROTOZOAN; RECOMBINATION; REPRODUCTION;

ANIMAL CELL; ARTICLE; CHICKEN; CONTROLLED STUDY; EIMERIA TENELLA; ELECTROPORATION; GENE OVEREXPRESSION; GENOME ANALYSIS; IMMUNOGENICITY; LIFE CYCLE; NONHUMAN; OOCYST; PROTEIN EXPRESSION; RESTRICTION MAPPING; SOUTHERN BLOTTING; SPOROZOITE; STABLE TRANSFECTION;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BACTERIAL PROTEINS; CHICKENS; DNA RESTRICTION ENZYMES; EIMERIA TENELLA; LIFE CYCLE STAGES; LUMINESCENT PROTEINS; OOCYSTS; PROTOZOAN VACCINES; TRANSFECTION;

EIMERIA; EIMERIA TENELLA; GALLUS GALLUS;

EID: 57549104381     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2008.06.013     Document Type: Article

References (38)

1. Black M., Seeber F., Soldati D., Kim K., and Boothroyd J.C. Restriction enzyme-mediated integration elevates transformation frequency and enables co-transfection of Toxoplasma gondii. Mol. Biochem. Parasitol. 74 (1995) 55-63
2. Bonilla J.A., Moura P.A., Bonilla T.D., Yowell C.A., Fidock D.A., and Dame J.B. Effects on growth, hemoglobin metabolism and paralogous gene expression resulting from disruption of genes encoding the digestive vacuole plasmepsins of Plasmodium falciparum. Int. J. Parasitol. 37 (2007) 317-327
3. Charest H., Sedegah M., Yap G.S., Gazzinelli R.T., Caspar P., Hoffman S.L., and Sher A. Recombinant attenuated Toxoplasma gondii expressing the Plasmodium yoelii circumsporozoite protein provides highly effective priming for CD8+ T cell-dependent protective immunity against malaria. J. Immunol. 165 (2000) 2084-2092
4. Danforth H.D. Use of live oocyst vaccines in the control of avian coccidiosis: experimental studies and field trials. Int. J. Parasitol. 28 (1998) 1099-1109
5. Digby M.R., and Lowenthal J.W. Cloning and expression of the chicken interferon-gamma gene. J. Interferon Cytokine Res. 15 (1995) 939-945
6. Doran D.J. Effect of age and freezing on development of Eimeria adenoeides and E. tenella sporozoites in cell culture. J. Parasitol. 56 (1970) 27-29
7. Goonewardene R., Daily J., Kaslow D., Sullivan T.J., Duffy P., Carter R., Mendis K., and Wirth D. Transfection of the malaria parasite and expression of firefly luciferase. Proc. Natl. Acad. Sci. USA 90 (1993) 5234-5236
8. Gubbels M.J., Li C., and Striepen B. High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein. Antimicrob. Agents Chemother. 47 (2003) 309-316
9. Hao L., Liu X., Zhou X., Li J., and Suo X. Transient transfection of Eimeria tenella using yellow or red fluorescent protein as a marker. Mol. Biochem. Parasitol. 153 (2007) 213-215
10. Haug A., Williams R.B., and Larsen S. Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method. Vet. Parasitol. 136 (2006) 233-242
11. Hofmann J., and Raether W. Improved techniques for the in vitro cultivation of Eimeria tenella in primary chick kidney cells. Parasitol. Res. 76 (1990) 479-486
12. Hong Y.H., Lillehoj H.S., Lillehoj E.P., and Lee S.H. Changes in immune-related gene expression and intestinal lymphocyte subpopulations following Eimeria maxima infection of chickens. Vet. Immunol. Immunopathol. 114 (2006) 259-272
13. Janse C.J., Franke-Fayard B., and Waters A.P. Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei. Nat. Protoc. 1 (2006) 614-623
14. Jeffers T.K. Attenuation of Eimeria tenella through selection for precociousness. J. Parasitol. 61 (1975) 1083-1090
15. Kelleher M., and Tomley F.M. Transient expression of beta-galactosidase in differentiating sporozoites of Eimeria tenella. Mol. Biochem. Parasitol. 97 (1998) 21-31
16. Kim K., and Weiss L.M. Toxoplasma gondii: the model apicomplexan. Int. J. Parasitol. 34 (2004) 423-432
17. Lee E.H. Single and low-level oocyst infections of drug-resistant field strains of Eimeria tenella in medicated birds. Can. Vet. J. 20 (1979) 102-104
18. Lillehoj H.S., and Choi K.D. Recombinant chicken interferon-gamma-mediated inhibition of Eimeria tenella development in vitro and reduction of oocyst production and body weight loss following Eimeria acervulina challenge infection. Avian Dis. 42 (1998) 307-314
19. Long P.L., Millard B.J., Joyner L.P., and Norton C.C. A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia. Vet. Lat. 6 (1976) 201-217
20. Long P.L. The Biology of the Coccidia (1982), Edward Arnold Press, London
21. Long P.L., and Rowell J.G. Counting oocysts of chicken coccidia. Lab. Pract. 7 (1958) 515-519
22. McDonald V., and Ballingall S. Further investigation of the pathogenicity, immunogenicity and stability of precocious Eimeria acervulina. Parasitology 86 Pt. 3 (1983) 361-369
23. McDonald V., Shirley M.W., and Bellatti M.A. Eimeria maxima: characteristics of attenuated lines obtained by selection for precocious development in the chicken. Exp. Parasitol. 61 (1986) 192-200
24. Norton C.C., Marshall S.H.S.N., Joyner L.P., and Parsons G. Freedom of coccidial oocysts from Newcastle disease virus. Res. Vet. Sci. 35 (1983) 367-369
25. Sambrook J., and Russell D. Molecular Cloning: A Laboratory Manual. third edition (2001), Cold Spring Harbor Labor Press, New York
26. Schmatz D.M., Crane M.S., and Murray P.K. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J. Protozool. 31 (1984) 181-183
27. Sharma J.M. Avian Cellular Immunology (1991), CRC Press, Boca Raton
28. Shedlock D.J., and Shen H. Requirement for CD4 T cell help in generating functional CD8 T cell memory. Science 300 (2003) 337-339
29. Shirley M.W. The genome of Eimeria spp., with special reference to Eimeria tenella-a coccidium from the chicken. Int. J. Parasitol. 30 (2000) 485-493
30. Sibley L.D., Messina M., and Niesman I.R. Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Proc. Natl. Acad. Sci. USA 91 (1994) 5508-5512
31. Soldati D., and Boothroyd J.C. Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii. Science 260 (1993) 349-352
32. Suo X., Zhang J.X., Li Z.G., Yang C.T., Min Q.R., Xu L.T., Liu Q., and Zhu X.Q. The efficacy and economic benefits of Supercox, a live anticoccidial vaccine in a commercial trial in broiler chickens in China. Vet. Parasitol. 142 (2006) 63-70
33. Tierney J., and Mulcahy G. Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro. Parasitol. Res. 90 (2003) 301-304
34. Trout J.M., and Lillehoj H.S. T lymphocyte roles during Eimeria acervulina and Eimeria tenella infections. Vet. Immunol. Immunopathol. 53 (1996) 163-172
35. van den Hoff J.B.M.F.M., and Lamers W.H. Electroporation in intracellular buffers increases cell survival. Nucleic Acid Res. 20 (1992) 2902
36. van Dijk M.R., Waters A.P., and Janse C.J. Stable transfection of malaria parasite blood stages. Science 268 (1995) 1358-1362
37. Williams R.B. Epidemiological aspects of the use of live anticoccidial vaccines for chickens. Int. J. Parasitol. 28 (1998) 1089-1098
38. Yun C.H., Lillehoj H.S., and Choi K.D. Eimeria tenella infection induces local gamma interferon production and intestinal lymphocyte subpopulation changes. Infect. Immun. 68 (2000) 1282-1288