Transient expression of β-galactosidase in differentiating sporozoites of Eimeria tenella

Molecular and Biochemical Parasitology

Volumn 97, Issue 1-2, 1998, Pages 21-31

  Kelleher, Michelle ^a,b   Tomley, Fiona M ^a  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

^b EDWARD JENNER INST FOR VACC RES   (United Kingdom)

Author keywords

Galactosidase; Differentiation; Eimeria; Etmic 1 gene; Micronemes; Transfection

Indexed keywords

BETA GALACTOSIDASE; REPORTER ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CHEMOLUMINESCENCE; COCCIDIOIDES; CONTROLLED STUDY; EIMERIA TENELLA; GENE EXPRESSION; GENETIC TRANSFECTION; MEROZOITE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCHIZONT; SPOROZOITE;

ANIMALS; ARTIFICIAL GENE FUSION; BASE SEQUENCE; BETA-GALACTOSIDASE; CELL LINE; CHEMILUMINESCENT MEASUREMENTS; EIMERIA TENELLA; ELECTROPORATION; GENES, REPORTER; LIFE CYCLE STAGES; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; TIME FACTORS; TRANSFECTION;

APICOMPLEXA; EIMERIA; EIMERIA TENELLA; ESCHERICHIA COLI;

EID: 0032583197     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(98)00128-5     Document Type: Article

References (24)

1. Trees A.J. Parasitic diseases. Jordan F.T.W., Pattison M. Poultry Diseases. 1996;261-289 Saunders, Philadelphia, PA.
2. Soldati D., Boothroyd J.C. Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii. Science. 260:1993;349-352.
3. Kim K., Soldati D., Boothroyd J.C. Gene replacement in Toxoplasma gondii with chloroamphenicol acetyltransferase as selectable marker. Science. 262:1993;911-914.
4. Donald R.G.K., Roos D.S. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proc Natl Acad Sci USA. 90:1993;11703-11707.
5. Sibley L.D., Messina M., Niesman I.R. Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Proc Natl Acad Sci USA. 91:1994;5508-5512.
6. Roos D.S., Donald R.G.K., Morrisette N.S., Moulton A.L.C. Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods Cell Biol. 45:1994;27-63.
7. Soldati D. Molecular genetic strategies in Toxoplasma gondii: close in on a successful invader. FEBS Lett. 389:1996;80-83.
8. Goodewardene R., Daily J., Kaslow D., Sullivan T.J., Duffy P., Carter R., Mendis K., Wirth D. Transfection of the malaria parasite and expression of firefly luciferase. Proc Natl Acad Sci USA. 90:1993;5234-5236.
9. van Dijk M.R., Waters A.P., Janse C.J. Stable transfection of malaria parasite blood stages. Science. 268:1995;1358-1362.
10. van Dijk M.R., Janse C.J., Waters A.P. Expression of a Plasmodium gene introduced into subtelomeric regions of Plasmodium berghei chromosomes. Science. 271:1996;662-665.
11. Wu Y., Sifri C.D., Lei H-H, Su X-Z, Wellems T.E. Transfection of Plasmodium falciparum within human red blood cells. Proc Natl Acad Sci USA. 92:1995;973-977.
12. Crabb B.S., Cowman A.F. Characterisation of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci USA. 93:1996;7289-7294.
13. Wu Y., Kirkman A.P., Wellems T.E. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad Sci USA. 93:1996;1130-1134.
14. Doran D.J. Behaviour of Coccidia in vitro. Long P.L. The Biology of the Coccidia. 1982;229-285 University Park Press, Baltimore.
15. Tomley F.M., Clarke L.E., Kawazoe U., Dijkema R., Kok J.J. Sequence of the gene encoding an immunodominant microneme protein of Eimeria tenella. Mol Biochem Parasitol. 49:1991;277-288.
16. LeBowitz J.H., Coburn C.M., Beverley S.M. Simultaneous transient expression assays adapted for the trypanosomatid parasite Leishmania using beta-galactosidase and beta-glucuronidase as reporter enzymes. Gene. 103:1991;119-123.
17. Seeber F., Boothroyd J.C. Escherichia coli β-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite, Toxoplasma gondii. Gene. 169:1996;39-45.
18. Long P.L., Joyner L.N., Millard B.J., Norton C.C. A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Fol Vet Lat. 6:1976;201-217.
19. McDougald L.R., Jeffers T.K. Comparative in vitro development of precocious and normal strains of Eimeria tenella (Coccidia). J Protozool. 23:1976;530-534.
20. Schmatz D.M., Crane MSt.J, Murray P.K. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J Protozool. 31:1984;181-183.
21. van den Hoff J.B., Moorman F.M., Lamers W.H. Electroporation in 'intracellular' buffers increases cell survival. Nuc Acid Res. 20:1992;2902.
22. MacGregor G.R., Nolan G.P., Fiering S., Roederer M., Herzenberg L.A. Use of E. coli lacZ (β-galactosidase) as a reporter gene. Methods Mol Biol. 7:1991;217-235.
23. Doran D.J. Increasing the yield of Eimeria tenella oocysts in cell culture. J Parasitol. 57:1971;891-900.
24. Tomley F.M., Bumstead J.M., Billington K.J., Dunn P.P.J. Molecular cloning and characterisation of a novel acidic microneme protein (Etmic-2) from the apicomplexan protozoan parasite, Eimeria tenella. Mol Biochem Parasitol. 79:1996;195-206.