Epsilonproteobacteria represent the major portion of chemoautotrophic bacteria in sulfidic waters of pelagic redoxclines of the baltic and black seas

Applied and Environmental Microbiology

Volumn 74, Issue 24, 2008, Pages 7546-7551

  Grote, Jana ^a   Jost, Günter ^a   Labrenz, Matthias ^a   Herndl, Gerhard J ^b   Jürgens, Klaus ^a  

^a LEIBNIZ INSTITUTE FOR BALTIC SEA RESEARCH   (Germany)

^b ROYAL NETHERLANDS INSTITUTE FOR SEA RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; FLUORESCENCE MICROSCOPY;

ABSOLUTE NUMBERS; AUTOTROPHIC DENITRIFICATIONS; BALTIC SEAS; BLACK SEAS; CELL ABUNDANCES; CHEMOAUTOTROPHIC BACTERIA; DO-MAINS; FIXATION RATES; FLUORESCENCE IN SITU HYBRIDIZATIONS; MICROAUTORADIOGRAPHY; PROKARYOTIC CELLS; REDOXCLINE; SULFIDIC WATERS;

CARBON DIOXIDE;

ABUNDANCE; BACTERIUM; CARBON DIOXIDE; CARBON FIXATION; DENITRIFICATION; PELAGIC ECOSYSTEM; POPULATION DISTRIBUTION; SULFIDE;

ARTICLE; AUTORADIOGRAPHY; BALTIC SEA; CHEMOAUTOTROPH; DENITRIFICATION; EPSILONPROTEOBACTERIA; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN; PELAGIC ZONE;

AUTORADIOGRAPHY; BIODIVERSITY; CARBON DIOXIDE; CARBON RADIOISOTOPES; COLONY COUNT, MICROBIAL; DARKNESS; EPSILONPROTEOBACTERIA; IN SITU HYBRIDIZATION, FLUORESCENCE; SEAWATER;

ATLANTIC OCEAN; BALTIC SEA; BLACK SEA; EURASIA;

BACTERIA (MICROORGANISMS); EPSILONPROTEOBACTERIA; PROKARYOTA; SULFURIMONAS;

EID: 57449085340     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01186-08     Document Type: Article

References (45)

1. Alonso, C., and J. Pernthaler. 2005. Incorporation of glucose under anoxic conditions by bacterioplankton from coastal North Sea surface waters. Appl. Environ. Microbiol. 71:1709-1716.
2. Amann, R., and B. M. Fuchs. 2008. Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques. Nat. Rev. Microbiol. 6:339-348.
3. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
4. Brettar, I., M. Labrenz, S. Flavier, J. Bötel, H. Kuosa, R. Christen, and M. G. Höfle. 2006. Identification of a Thiomicrospira denitrificans-like epsilonproteobacterium as a catalyst for autotrophic denitrification in the central Baltic Sea. Appl. Environ. Microbiol. 72:1364-1372.
5. Campbell, B. J., A. S. Engel, M. L. Porter, and K. Takai. 2006. The versatile ?-proteobacteria: key players in sulphidic habitats. Nat. Rev. Microbiol. 4:458-468.
6. Casamayor, E. O., J. García-Cantizano, and C. Pedrós-Alió. 2008. Carbon dioxide fixation in the dark by photosynthetic bacteria in sulfide-rich stratified lakes with oxic-anoxic interfaces. Limnol. Oceanogr. 53:1193-1203.
7. Cottrell, M. T., and D. L. Kirchman. 2000. Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66:1692-1697.
8. Daims, H., A. Bruhl, R. Amann, K.-H. Schleifer, and M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22:434-444.
9. 2 incorporation among planktonic microbial guilds and estimation of in situ specific growth rates. Microb. Ecol. 50:230-241.
10. 13C-isotope analyses reveal that chemolithoautotrophic Gamma- and Epsilonproteobacteria feed a microbial food web in a pelagic redoxcline of the central Baltic Sea. Environ. Microbiol. doi:10.1111/j.1462-2920.2008.01770.x.
11. Grasshoff, K., M. Erhardt, and K. Kremling. 1983. Methods of seawater analysis, vol. 2. Verlag Chemie, Weinheim, Germany.
12. Gray, N. D., R. Howarth, R. W. Pickup, J. G. Jones, and I. M. Head. 2000. Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. Appl. Environ. Microbiol. 66:4518-4522.
13. Grote, J., M. Labrenz, B. Pfeiffer, G. Jost, and K. Jürgens. 2007. Quantitative distributions of Epsilonproteobacteria and a Sulfurimonas subgroup in pelagic redoxclines of the central Baltic Sea. Appl. Environ. Microbiol. 73:7155-7161.
14. Hadas, O., R. Pinkas, and J. Erez. 2001. High chemoautotrophic primary production in Lake Kinneret, Israel: a neglected link in the carbon cycle of the lake. Limnol. Oceanogr. 46:1968-1976.
15. Herndl, G. J., T. Reinthaler, E. Teira, H. van Aken, C. Veth, A. Pernthaler, and J. Pernthaler. 2005. Contribution of Archaea to total prokaryotic production in the deep Atlantic Ocean. Appl. Environ. Microbiol. 71:2303-2309.
16. Ingalls, A. E., S. R. Shah, R. L. Hansman, L. I. Aluwihare, G. M. Santos, E. R. M. Druffel, and A. Pearson. 2006. Quantifying archaeal community autotrophy in the mesopelagic ocean using natural radiocarbon. Proc. Natl. Acad. Sci. USA 103:6442-6447.
17. Johnson, K. M., K. D. Wills, D. B. Butler, W. K. Johnson, and C. S. Wong. 1993. Coulometric total carbon dioxide analysis for marine studies: maximizing the performance of an automated gas extraction system and coulometric detector. Mar. Chem. 44:167-187.
18. Jørgensen, B. B., H. Fossing, C. O. Wirsen, and H. W. Jannasch. 1991. Sulfide oxidation in the anoxic Black Sea chemocline. Deep-Sea Res. 38: S1083-S1103.
19. 2 fixation of chemolithoautotrophic prokaryotes in anoxic waters of the Baltic Sea. Limnol. Oceanogr. 53:14-22.
20. Kirchman, D. L., H. Elifantz, A. I. Dittel, R. R. Malmstrom, and M. T. Cottrell. 2007. Standing stocks and activity of archaea and bacteria in the western Arctic Ocean. Limnol. Oceanogr. 52:495-507.
21. Labrenz, M., G. Jost, C. Pohl, S. Beckmann, W. Martens-Habbena, and K. Jürgens. 2005. Impact of different in vitro electron donor/acceptor conditions on potential chemolithoautotrophic communities from marine pelagic redoxclines. Appl. Environ. Microbiol. 71:6664-6672.
22. Lee, N., P. H. Nielsen, K. H. Andreasen, S. Juretschko, J. L. Nielsen, K. H. Schleifer, and M. Wagner. 1999. Combination of fluorescent in situ hybridization and microautoradiography - a new tool for structure-function analyses in microbial ecology. Appl. Environ. Microbiol. 65:1289-1297.
23. Lin, X., M. I. Scranton, R. Varela, A. Chistoserdov, and G. T. Taylor. 2007. Compositional responses of bacterial communities to redox gradients and grazing in the anoxic Cariaco Basin. Aquat. Microb. Ecol. 47:57-72.
24. Lin, X., S. G. Wakeham, I. F. Putnam, Y. M. Astor, M. I. Scranton, A. Y. Chistoserdov, and G. T. Taylor. 2006. Comparison of vertical distributions of prokaryotic assemblages in the anoxic Cariaco Basin and Black Sea by use of fluorescence in situ hybridization. Appl. Environ. Microbiol. 72:2679-2690.
25. Loy, A., F. Maixner, M. Wagner, and M. Horn. 2007. probeBase - an online resource for rRNA-targeted oligonucleotide probes: new features 2007. Nucleic Acids Res. 35:D800-D804.
26. Madrid, V. M., G. T. Taylor, M. I. Scranton, and A. Y. Chistoserdov. 2001. Phylogenetic diversity of bacterial and archaeal communities in the anoxic zone of the Cariaco Basin. Appl. Environ. Microbiol. 67:1663-1674.
27. 2 fixation rates at deep-sea hydrothermal vents and the oxic/anoxic interface in Framvaren Fjord, Norway. Mar. Chem. 66:201-213.
28. Manz, W., R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer. 1992. Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15:593-600.
29. Medina-Sánchez, J. M., M. Felip, and E. O. Casamayor. 2005. Catalyzed reported deposition-fluorescence in situ hybridization protocol to evaluate phagotrophy in mixotrophic protists. Appl. Environ. Microbiol. 71:7321-7326.
30. Nakagawa, S., K. Takai, F. Inagaki, H. Hirayama, T. Nunoura, K. Horikoshi, and Y. Sako. 2005. Distribution, phylogenetic diversity and physiological characteristics of epsilon-Proteobacteria in a deep-sea hydrothermal field. Environ. Microbiol. 7:1619-1632.
31. Nielsen, J. L., D. Christensen, M. Kloppenborg, and P. H. Nielsen. 2003. Quantification of cell-specific substrate uptake by probe-defined bacteria under in situ conditions by microautoradiography and fluorescence in situ hybridization. Environ. Microbiol. 5:202-211.
32. 2 fixation and sulfur cycling associated with low-temperature emissions at the Lilliput hydrothermal field, southern Mid-Atlantic Ridge (9°S). Environ. Microbiol. 9:1186-1201.
33. Pernthaler, A., J. Pernthaler, and R. Amann. 2002. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Appl. Environ. Microbiol. 68:3094-3101.
34. Pimenov, N. V., and L. N. Neretin. 2006. Composition and activities of microbial communities involved in carbon, sulfur, nitrogen and manganese cycling in the oxic/anoxic interface of the Black Sea, p. 501-521. In L. N. Neretin (ed.), Past and present marine water column anoxia. Springer, Dordrecht, The Netherlands.
35. Sekar, R., A. Pernthaler, J. Pernthaler, F. Warnecke, T. Posch, and R. Amann. 2003. An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization. Appl. Environ. Microbiol. 69:2928-2935.
36. Sorokin, J. I. 1964. On the primary production and bacterial activities in the Black Sea. J. Council Explor. Mer. 29:41-60.
37. Sorokin, Y. I. 1972. The bacterial population and the processes of hydrogen sulphide oxidation in the Black Sea. J. Council Explor. Mer. 34:423-454.
38. Takai, K., F. Inagaki, S. Nakagawa, H. Hirayama, T. Nunoura, Y. Sako, K. H. Nealson, and K. Horikoshi. 2003. Isolation and phylogenetic diversity of members of previously uncultivated ε-proteobacteria in deep-sea hydrothermal fields. FEMS Microbiol. Lett. 218:167-174.
39. Taylor, G. T., M. Iabichella-Armas, R. Varela, F. Müller-Karger, X. Lin, and M. I. Scranton. 2006. Microbial ecology of the Cariaco Basin's redoxcline: the U.S.-Venezuela Cariaco times series program, p. 473-499. In L. N. Neretin (ed.), Past and present marine water column anoxia. Springer, Dordrecht, The Netherlands.
40. Taylor, G. T., M. Iabichella, T.-Y. Ho, M. I. Scranton, R. C. Thunell, F. Müller-Karger, and R. Varela. 2001. Chemoautotrophy in the redox transition zone of the Cariaco Basin: a significant midwater source of organic carbon production. Limnol. Oceanogr. 46:148-163.
41. Teira, E., T. Reinthaler, A. Pernthaler, J. Pernthaler, and G. J. Herndl. 2004. Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean. Appl. Environ. Microbiol. 70:4411-4414.
42. 2 in the Cariaco Trench. Limnol. Oceanogr. 24:746-753.
43. Vetriani, C., H. V. Tran, and L. J. Kerkhof. 2003. Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea. Appl. Environ. Microbiol. 69:6481-6488.
44. Wallner, G., R. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.
45. Zopfi, J., T. G. Ferdelman, B. B. Jørgensen, A. Teske, and B. Thamdrup. 2001. Influence of water column dynamics on sulfide oxidation and other major biogeochemical processes in the chemocline of Mariager Fjord (Denmark). Mar. Chem. 74:29-51.