Apoptotic insults to human HepG2 cells induced by S-(+)-ketamine occurs through activation of a Bax-mitochondria-caspase protease pathway

British Journal of Anaesthesia

Volumn 102, Issue 1, 2009, Pages 80-89

  Lee, S T ^a,b   Wu, T T ^b,d   Yu, P Y ^c,e   Chen, R M ^b,c  

^a CATHAY GENERAL HOSPITAL   (Taiwan)

^b TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^c TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^d Taipei County Hospital   (Taiwan)

^e TAIPEI CITY HOSPITAL   (Taiwan)

Author keywords

Anaesthetics i.v., ketamine; Liver, hepatotoxicity; Metabolism, ATP, DNA; Theories of anaesthetic action, cellular mechanisms

Indexed keywords

ADENOSINE TRIPHOSPHATE; BETA ACTIN; CASPASE; CASPASE 3; CASPASE 6; CASPASE 9; CASPASE INHIBITOR; CYTOCHROME C; DNA FRAGMENT; GAMMA GLUTAMYLTRANSFERASE; KETAMINE; LACTATE DEHYDROGENASE; PROTEIN BAX; PROTEINASE;

APOPTOSIS; ARTICLE; BIOLUMINESCENCE; CELL STRAIN HEPG2; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; DRUG EXPOSURE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME RELEASE; FLUOROMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIVER DYSFUNCTION; LIVER TOXICITY; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; TIME;

EID: 57349180886     PISSN: 00070912     EISSN: 14716771     Source Type: Journal    
DOI: 10.1093/bja/aen322     Document Type: Article

References (35)

1. White PF, Way WL, Trevor AJ. Ketamine: its pharmacology and therapeutic uses. Anesthesiology 1982; 56: 119-36
2. Lankenau SE, Sanders B, Bloom JJ, et al. First injection of ketamine among young injection drug users (IDUs) in three U.S. cities. Drug Alcohol Depend 2007; 87: 183-9
3. Schmidt H, Ebeling D, Bauer H, et al. Ketamine attenuates endotoxin-induced leukocyte adherence in rat mesenteric venules. Crit Care Med 1995; 23: 2008-14
4. Krumholz W, Endrass J, Hempelmann G. Inhibition of phagocytosis and killing of bacteria by anaesthetic agents in vitro. Br J Anaesth 1995; 75: 66-70
5. Chang Y, Chen TL, Sheu JR, et al. Suppressive effects of ketamine on macrophage functions. Toxicol Appl Pharmacol 2005; 204: 27-35
6. Chen RM, Chen TL, Lin YL, et al. Ketamine reduces nitric oxide biosynthesis in human umbilical vein endothelial cells through downregulating endothelial nitric oxide synthase expression and intracellular calcium levels. Crit Care Med 2005; 33: 1044-9
7. Navarro VJ, Senior JR. Drug-related hepatotoxicity. N Engl J Med 2006; 354: 731-9
8. Papeleu P, Vanhaecke T, Elaut G, et al. Differential effects of histone deacetylase inhibitors in tumor and normal cells-what is the toxicological relevance? Crit Rev Toxicol 2005; 35: 363-70
9. Ku NO, Strnad P, Zhong BH, et al. Keratins let liver live: mutations predispose to liver disease and crosslinking generates Mallory-Denk bodies. Hepatology 2007; 46: 1639-49
10. Chan WH, Sun WZ, Ueng TH. Induction of rat hepatic cytochrome P-450 by ketamine and its toxicological implications. J Toxicol Environ Health 2005; 68: 1581-97
11. Kimura F, Hashimoto Y, Shimodate Y, et al. Clinical study on total intravenous anesthesia with droperidol, fentanyl and ketamine. Hepatic and renal functions following prolonged surgical operation of over 10 h. Masui 1991; 40: 1371-5
12. Hijazi Y, Boulieu R. Contribution of CYP3A4, CYP2B6, and CYP2C9 isoforms to N-demethylation of ketamine in human liver microsomes. Drug Metab Dispos 2002; 30: 853-8
13. Dawson JR, Adams DJ, Wolf CR, et al. Induction of drug metabolizing enzymes in human liver cell line HepG2. FEBS Lett 1985; 183: 219-22
14. Chen RM, Chou MW, Ueng TH. Induction of cytochrome P450 1A1 in human hepatoma HepG2 cells by 6-nitrochrysene. Toxicol Lett 2000; 117: 69-77
15. Cobb JP, Hotchkiss RS, Karl IE, Buchman TG. Mechanisms of cell injury and death. Br J Anaesth 1996; 77: 3-10
16. Chen RM, Chen TL, Chiu WT, et al. Molecular mechanism of nitric oxide-induced osteoblast apoptosis. J Orthop Res 2005; 23: 462-8
17. Hortelano S, Alvarez AM, Bosca L. Nitric oxide induces tyrosine nitration and release of cytochrome c preceding an increase of mitochondrial transmembrane potential in macrophages. FASEB J 1999; 13: 2311-7
18. Blom WM, de Bont HJ, Nagelkerke JF. Regional loss of the mitochondrial membrane potential in the hepatocyte is rapidly followed by externalization of phosphatidylserines at that specific site during apoptosis. J Biol Chem 2003; 278: 12467-74
19. Cherng YG, Chang HC, Lin YL, et al. Apoptotic insults to human chondrocytes induced by nitric oxide are involved in sequential events, including cytoskeletal remodeling, phosphorylation of mitogen-activated protein kinase kinase kinase-1, and Baxmitochondria-mediated caspase activation. J Orthop Res 2008; 26: 1018-26
20. Wang C, Sadovova N, Fu X, et al. The role of the N-methyl-D-aspartate receptor in ketamine-induced apoptosis in rat forebrain culture. Neuroscience 2005; 132: 967-77
21. Chen RM, Chou MW, Ueng TH. Induction of cytochrome P450 1A in hamster liver and lung by 6-nitrochrysene. Arch Toxicol 1998; 72: 395-401
22. Tai YT, Chen TL, Cherng YG, et al. Pretreatment with low nitric oxide protects osteoblasts from high nitric oxide-induced apoptotic insults through regulation of c-Jun N-terminal kinase/ c-Jun-mediated Bcl-2 gene expression and protein translocation. J Orthop Res 2007; 25: 625-35
23. Chen TG, Chen TL, Chang HC, et al. Oxidized low-density lipoprotein induces apoptotic insults to mouse cerebral endothelial cells via a Bax-mitochondria-caspase protease pathway. Toxicol Appl Pharmacol 2007; 219: 42-53
24. Chang CC, Liao YS, Lin YL, et al. Nitric oxide protects osteoblasts from oxidative stress-induced apoptotic insults via a mitochondria-dependent mechanism. J Orthop Res 2006; 24: 1917-25
25. Chen RM, Wu CH, Chang HC, et al. Propofol suppresses macrophage functions through modulating mitochondrial membrane potential and cellular adenosine triphosphate levels. Anesthesiology 2003; 98: 1178-85
26. Domino EF, Zsigmond EK, Domino LE, et al. Plasma levels of ketamine and two of its metabolites in surgical patients using a gas chromatographic mass fragmentographic assay. Anesth Analg 1982; 61: 87-92
27. Larenza MP, Landoni MF, Levionnois OL, et al. Stereoselective pharmacokinetics of ketamine and norketamine after racemic ketamine or S-ketamine administration during isoflurane anaesthesia in Shetland ponies. Br J Anaesth 2007; 98: 204-12
28. Chen RM, Lin YL, Jean WC, et al. Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein. J Orthop Res 2002; 20: 295-302
29. Schmid I, Uittenbogaart CH, Giorgi JV. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 1994; 15: 12-20
30. Hsu YT, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA 1997; 94: 3668-72
31. Saikumar P, Dong Z, Patel Y, et al. Role of hypoxia-induced Bax translocation and cytochrome c release in reoxygenation injury. Oncogene 1998; 17: 3401-15
32. Kagan VE, Borisenko GG, Tyurina YY, et al. Oxidative lipidomics of apoptosis: redox catalytic interactions of cytochrome c with cardiolipin and phosphatidylserine. Free Rad Biol Med 2004; 37: 1963-85
33. Goyal L. Cell death inhibition: keeping caspases in check. Cell 2001; 104: 805-8
34. Rao L, Perez D, White E. Lamin proteolysis facilitates nuclear events during apoptosis. J Cell Biol 1996; 135: 1441-55
35. Clements JA, Nimmo WS. Pharmacokinetics and analgesic effect of ketamine in man. Br J Anaesth 1981; 53: 27-30