Auto-activation mechanism of the Mycobacterium tuberculosis PknB receptor Ser/Thr kinase

EMBO Journal

Volumn 27, Issue 23, 2008, Pages 3186-3197

  Mieczkowski, Carl ^a   Iavarone, Anthony T ^b   Alber, Tom ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Autophosphorylation; Bacterial protein kinase; G helix

Indexed keywords

ADENOSINE TRIPHOSPHATE; PKNB KINASE; PROTEIN SERINE THREONINE KINASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; AUTOPHOSPHORYLATION; AUTORADIOGRAPHY; BINDING SITE; CHEMICAL MODIFICATION; CRYSTAL STRUCTURE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY;

AMINO ACID SUBSTITUTION; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ENZYME INHIBITORS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION, MISSENSE; MYCOBACTERIUM TUBERCULOSIS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN-SERINE-THREONINE KINASES;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS;

EID: 57149120752     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/emboj.2008.236     Document Type: Article

References (35)

1. Av-Gay Y, Everett M (2000) The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Trends Microbiol 8: 238-244
2. Biondi RM, Nebreda AR (2003) Signalling specificity of Ser/Thr protein kinases through docking-site-mediated interactions. Biochem J 372: 1-13
3. Boitel B, Ortiz-Lombardia M, Duran R, Pompeo F, Cole ST, Cervenansky C, Alzari PM (2003) PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis. Mol Microbiol 49: 1493-1508
4. Dar AC, Dever TE, Sicheri F (2005) Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR. Cell 122: 887-900
5. DeLano WL (2002) The PyMOL Molecular Graphics System. San Carlos, CA: DeLano Scientific
6. Dey M, Cao C, Dar AC, Tamura T, Ozato K, Sicheri F, Dever TE (2005) Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition. Cell 122: 901-913
7. Duran R, Villarino A, Bellinzoni M, Wehenkel A, Fernandez P, Boitel B, Cole ST, Alzari PM, Cervenansky C (2005) Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases. Biochem Biophys Res Commun 333: 858-867
8. Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60: 2126-2132
9. Gay LM, Ng HL, Alber T (2006) A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosis. J Mol Biol 360: 409-420
10. Greenstein AE, Echols N, Lombana TN, King DS, Alber T (2007a) Allosteric activation by dimerization of the PknD receptor Ser/ Thr protein kinase from Mycobacterium tuberculosis. J Biol Chem 282: 11427-11435
11. Greenstein AE, Grundner C, Echols N, Gay LM, Lombana TN, Miecskowski CA, Pullen KE, Sung PY, Alber T (2005) Structure/function studies of Ser/Thr and Tyr protein phosphorylation in Mycobacterium tuberculosis. J Mol Microbiol Biotechnol 9: 167-181
12. Greenstein AE, MacGurn JA, Baer CE, Falick AM, Cox JS, Alber T (2007b) M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. PLoS Pathog 3: e49
13. Holton J, Alber T (2004) Automated protein crystal structure determination using ELVES. Proc Natl Acad Sci USA 101: 1537-1542
14. Huse M, Kuriyan J (2002) The conformational plasticity of protein kinases. Cell 109: 275-282
15. Kang CM, Abbott DW, Park ST, Dascher CC, Cantley LC, Husson RN (2005) The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev 19: 1692-1704
16. Knight ZA, Schilling B, Row RH, Kenski DM, Gibson BW, Shokat KM (2003) Phosphospecific proteolysis for mapping sites of protein phosphorylation. Nat Biotechnol 21: 1047-1054
17. Krissinel E, Henrick K (2004) Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions. Acta Crystallogr D Biol Crystallogr 60: 2256-2268
18. Laskowski RA, MacArthur MW, Moss DS, Thornton JM (1993) PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Crystallog 26: 283-291
19. Lawrie AM, Noble ME, Tunnah P, Brown NR, Johnson LN, Endicott JA (1997) Protein kinase inhibition by staurosporine revealed in details of the molecular interaction with CDK2. Nat Struct Biol 4: 796-801
20. Madhusudan, Akamine P, Xuong NH, Taylor SS (2002) Crystal structure of a transition state mimic of the catalytic subunit of cAMP-dependent protein kinase. Nat Struct Biol 9: 273-277
21. Marshall AG, Hendrickson CL, Shi SD (2002) Scaling MS plateaus with high-resolution FT-ICRMS. Anal Chem 74: 252A-259A
22. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53: 240-255
23. Oliver AW, Paul A, Boxall KJ, Barrie SE, Aherne GW, Garrett MD, Mittnacht S, Pearl LH (2006) Trans-activation of the DNA-damage signalling protein kinase Chk2 by T-loop exchange. EMBO J 25: 3179-3190
24. Ortiz-Lombardia M, Pompeo F, Boitel B, Alzari PM (2003) Crystal structure of the catalytic domain of the PknB serine/ threonine kinase from Mycobacterium tuberculosis. J Biol Chem 278: 13094-13100
25. Otwinowski Z, Minor W (1997) Processing of X-ray diffraction data collected in oscillation mode. In Macromolecular Crystallography Carter Jr CW, Sweet RM (eds), Vol. 276, pp 307-326. San Diego: Academic Press
26. Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S (2008) Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites. EMBO J 27: 704-714
27. Pirruccello M, Sondermann H, Pelton JG, Pellicena P, Hoelz A, Chernoff J, Wemmer DE, Kuriyan J (2006) A dimeric kinase assembly underlying autophosphorylation in the p21 activated kinases. J Mol Biol 361: 312-326
28. Potterton E, Briggs P, Turkenburg M, Dodson E (2003) A graphical user interface to the CCP4 program suite. Acta Crystallogr D Biol Crystallogr 59: 1131-1137
29. Roepstorff P, Fohlman J (1984) Proposal for a common nomenclature for sequence ions in mass spectra of peptides. Biomed Mass Spectrom 11: 601
30. Scherr N, Honnappa S, Kunz G, Mueller P, Jayachandran R, Winkler F, Pieters J, Steinmetz MO (2007) Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Proc Natl Acad Sci USA 104: 12151-12156
31. Schuttelkopf AW, van Aalten DM (2004) PRODRG: a tool for high-throughput crystallography of protein-ligand complexes. Acta Crystallogr D Biol Crystallogr 60: 1355-1363
32. Storoni LC, McCoy AJ, Read RJ (2004) Likelihood-enhanced fast rotation functions. Acta Crystallogr D Biol Crystallogr 60: 432-438
33. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680
34. Wehenkel A, Fernandez P, Bellinzoni M, Catherinot V, Barilone N, Labesse G, Jackson M, Alzari PM (2006) The structure of PknB in complex with mitoxantrone, an ATP-competitive inhibitor, suggests a mode of protein kinase regulation in mycobacteria. FEBS Lett 580: 3018-3022
35. Young TA, Delagoutte B, Endrizzi JA, Falick AM, Alber T (2003) Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases. Nat Struct Biol 10: 168-174