Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases

Biochemical and Biophysical Research Communications

Volumn 333, Issue 3, 2005, Pages 858-867

  Durán, Rosario ^a   Villarino, Andrea ^b   Bellinzoni, Marco ^b   Wehenkel, Annemarie ^b   Fernandez, Pablo ^b   Boitel, Brigitte ^b   Cole, Stewart T ^b   Alzari, Pedro M ^b   Cerveñansky, Carlos ^a  

^a INSTITUTO DE INVESTIGACIONES BIOLÓGICAS CLEMENTE ESTABLE   (Uruguay)

^b INSTITUT PASTEUR   (France)

Author keywords

Activation loop; Bacterial signaling; Juxtamembrane region; Mass spectrometry; Mycobacterium tuberculosis; Phosphopeptide identification; Ser Thr kinase autophosphorylation

Indexed keywords

PHOSPHATASE; PROTEIN SERINE THREONINE KINASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; DEPHOSPHORYLATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL MEMBRANE; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MYCOBACTERIUM TUBERCULOSIS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

MYCOBACTERIUM TUBERCULOSIS;

EID: 20744451425     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.05.173     Document Type: Article

References (30)

1. T. Hunter Signaling-2000 and beyond Cell 100 2000 113 127
2. C.J. Bakal, and J.E. Davies No longer an exclusive club: eukaryotic signalling domains in bacteria Trends Cell Biol. 10 2000 32 38
3. A.M. Stock, V.L. Robinson, and P.N. Goudreau Two-component signal transduction Annu. Rev. Biochem. 69 2000 183 215
4. S. Hakansson, E.E. Galyov, R. Rosqvist, and H. Wolf-Watz The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane Mol. Microbiol. 20 1996 593 603
5. J. Wang, C. Li, H. Yang, A. Mushegian, and S. Jin A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice J. Bacteriol. 180 1998 6764 6768
6. E. Madec, A. Laszkiewicz, A. Iwanicki, M. Obuchowski, and S. Séror Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes Mol. Microbiol. 46 2002 571 586
7. K. Petrícková, and M. Petricek Eukaryotic-type protein kinases in Streptomyces coelicolor: variations on a common theme Microbiology 149 2003 1609 1621
8. A. Walburger, A. Koul, G. Ferrari, L. Nguyen, C. Prescianotto-Baschong, K. Huygen, B. Klebl, C. Thompson, G. Bacher, and J. Pieters Protein kinase G from pathogenic mycobacteria promotes survival within macrophages Science 304 2004 1800 1804
9. S.T. Cole, R. Brosch, J. Parkhill, and T. Garnier Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence Nature 393 1998 537 544
10. P. Peirs, L. De Wit, M. Braibant, K. Huygen, and J. Content A serine/threonine protein.kinase from Mycobacterium tuberculosis Eur. J. Biochem. 244 1997 604 612
11. Y. Av-Gay, S. Jamil, and S.J. Drews Expression and characterization of the Mycobacterium tuberculosis serine/threonine protein kinase PknB Infect. Immun. 67 1999 5676 5682
12. A. Koul, A. Choidas, A.K. Tyagi, K. Drlica, Y. Singh, and A. Ullrich Serine/threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization and localization Microbiology 147 2001 2307 2314
13. R. Chaba, M. Raje, and P.K. Chakraborti Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division Eur. J. Biochem. 269 2002 1078 1085
14. B. Boitel, M. Ortiz-Lombardía, R. Durán, F. Pompeo, S.T. Cole, C. Cerveñansky, and P.M. Alzari PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis Mol. Microbiol. 49 2003 1493 1508
15. T.A. Young, B. Delagoutte, J.A. Endrizzi, A.M. Falick, and T. Alber Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases Nat. Struct. Biol. 10 2003 168 174
16. V. Molle, C. Girard-Blanc, L. Kremer, P. Doublet, A.J. Cozzone, and J.F. Prost Protein PknE, a novel transmembrane eukaryotic-like serine/threonine kinase from Mycobacterium tuberculosis Biochem. Biophys. Res. Commun. 308 2003 820 825
17. P. Chopra, B. Singh, R. Singh, R. Vohra, A. Koul, L.S. Meena, H. Koduri, M. Ghildiyal, P. Deol, T.K. Das, A.K. Tyagi, and Y. Singh Phosphoprotein phosphatase of Mycobacterium tuberculosis dephosphorylates serine-threonine kinases PknA and PknB Biochem. Biophys. Res. Commun. 311 2003 112 120
18. R. Gopalaswamy, P.R. Narayanan, and S. Narayanan Cloning, overexpression, and characterization of a serine/threonine protein kinase pknI from Mycobacterium tuberculosis H37Rv Protein Expr. Purif. 36 2004 82 89
19. L.N. Johnson, M.E. Noble, and D.J. Owen Active and inactive protein kinases: structural basis for regulation Cell 85 1996 149 158
20. M. Huse, and J. Kuriyan The conformational plasticity of protein kinases Cell 109 2002 275 282
21. M. Mann, S.-E. Ong, M. Gronborg, H. Steen, O.N. Jensen, and A. Pandey Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome Trends Biotechnol. 20 2002 261 268
22. S.B. Ficarro, M.L. McCleland, P.T. Stukenberg, D.J. Burke, M.M. Ross, J. Shabanowitz, D.F. Hunt, and F.M. White Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae Nat. Biotechnol. 20 2002 301 305
23. K.A. Resing, R.S. Johnson, and K.A. Walsh Mass spectrometric analysis of 21 phosphorylation sites in the internal repeat of rat profilaggrin, precursor of an intermediate filament associated protein Biochemistry 34 1995 9477 9487
24. R.S. Annan, and S.A. Carr Phosphopeptide analysis by matrix-assisted laser desorption time-of-flight mass spectrometry Anal. Chem. 68 1996 3413 3421
25. M. Ortiz-Lombardía, F. Pompeo, B. Boitel, and P.M. Alzari Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis J. Biol. Chem. 278 2003 13094 13100
26. E. Madec, A. Stensballe, S. Kjellstrom, L. Cladiere, M. Obuchowski, O.N. Jensen, and S.J. Seror Mass spectrometry and site-directed mutagenesis identify several autophosphorylated residues required for the activity of PrkC, a Ser/Thr kinase from Bacillus subtilis J. Mol. Biol. 330 2003 459 472
27. D.R. Knighton, J.H. Zheng, L.F. Ten Eyck, V.A. Ashford, N.H. Xuong, S.S. Taylor, and J.M. Sowadski Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase Science 253 1991 407 414
28. A. Villarino, R. Duran, A. Wehenkel, P. Fernandez, P. England, P. Brodin, S.T. Cole, U. Zimny-Arnat, P.R. Jungblut, C. Cerveñansky, P.M. Alzari, Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA-containing protein, through activation loop-mediated interactions, J. Mol. Biol. (2005) in press.
29. M. Huse, Y.-G. Chen, J. Massague, and J. Kuriyan Crystal structure of the cytoplasmic domain of the type I TGF beta receptor in complex with FKBP12 Cell 96 1999 425 436
30. L.E Wybenga-Groot, B. Baskin, S.H. Ong, J. Tong, T. Pawson, and F. Sicheri Structural basis for autoinhibition of the Ephb2 receptor tyrosine kinase by the unphosphorylated juxtamembrane region Cell 106 2001 745 757