Detection of diarrheic shellfish poisoning and azaspiracid toxins in Moroccan mussels: Comparison of the LC-MS Method with the commercial immunoassay kit

Marine Drugs

Volumn 6, Issue 4, 2008, Pages 587-594

  Elgarch, Adra ^a   Vale, Paulo ^b   Rifai, Saida ^a   Fassouane, Aziz ^a  

^a FACULTÉ DES SCIENCES   (Morocco)

^b INSTITUTO PORTUGUÊS DO MAR E DA ATMOSFERA   (Portugal)

Author keywords

Azaspiracids toxins; Diarrheic shellfish poisoning; Dinophysis spp.; Dinophysistoxin 2; ELISA; LC MS; Okadaic acid

Indexed keywords

AZASPIRACID; DINOPHYSISTOXIN 2; ESTER; MARINE TOXIN; OKADAIC ACID; UNCLASSIFIED DRUG;

ANIMAL MODEL; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION ANALYSIS; DIARRHEIC SHELLFISH POISONING; DILUTION; DRUG CONTAMINATION; HYDROLYSIS; IMMUNOASSAY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOROCCO; MOUSE; MUSSEL; NONHUMAN; QUANTITATIVE ANALYSIS; SAMPLE; SEASHORE; SHELLFISH; SHELLFISH POISONING;

DINOPHYSIS;

EID: 57149117666     PISSN: None     EISSN: 16603397     Source Type: Journal    
DOI: 10.3390/md6040587     Document Type: Article

References (16)

1. Lee, J.S.; Yanaji, T.; Kenma, R.; Yasumoto; T. Fluorometric determination of diarrheic shellfish toxins by high performance liquid chromatography. Agric. Biol. Chem. 1987, 51, 877-881.
2. Fernandez, M.L.; Miguez, A.; Cacho, E.; Martinez, A. Detection of okadaic acid esters in the hexane extracts of Spanish mussels. Toxicon 1996, 34, 381-387.
3. Vale, P.; Sampayo, M.A.M. Comparison between HPLC and a commercial immunoassay kit for detection of okadaic acid and esters in Portuguese bivalves. Toxicon 1999, 37, 1565-1577.
4. Usagawa, T.; Nishimura, M.; Itoh., Y.; Uda, T.; Yasumoto, T. Preparation of monoclonal antibodies against okadaic acid prepared from the sponge Halichondria okadai. Toxicon 1989, 27, 1323-1330.
5. Vale, P., Sampayo, M.A.M. Esters of okadaic acid and Dinophysistoxin-2 in Portuguese bivalves related to human poisonings. Toxicon 1999, 37, 1109-1121.
6. Vale, P. Is there a risk of human poisoning by azaspiracids from shellfish harvested at Portuguese coast? Toxicon 2004, 44, 943-947.
7. Vale, P.; Sampayo, M.A.M. Dinophysistoxin-2: a rare diarrheic toxin associated with Dinophysis acuta. Toxicon 2000, 38, 1599-1606.
8. Taleb, H.; Vale, P.; Amanhir, R.; Benhadouch, A.; Sagou, R. First detection of azaspiracids in North West Africa. J. Shellfish Res. 2006, 25, 3.
9. James, K.J.; Furey, A.; Lehane, M.; Ramstad, H.; Aune, T.; Hovgaard, P.; Morris, S.; Higman, W.; Satake, M.; Yasumoto, T. First evidence of an extensive Northern European distribution of azaspiracid poisoning (AZP) toxins in shellfish. Toxicon 2002, 40, 909-915.
10. Magdalena, A.B.; Lehane, M.; Krys, S.; Fernandez, M.L.; Furey, A.; James, K.J. The first identification of azaspiracids in shellfish from France and Spain. Toxicon 2003, 42, 105-108.
11. James, K.J.; Moroney, C.; Roden, C.; Satake, M.; Yasumoto, T.; Lehane, M.; Furey, A. Ubiquitous benign algae emerges as the cause of shellfish contamination responsible for the human toxic syndrome, azaspiracid poisoning. Toxicon 2003, 41, 145-151.
12. James, K.J.; Sierra, M.D.; Lehane, M.; Magdalena, A.B.; Furey, A. Detection of five new hydroxyl analogues of azaspiracids in shellfish using multiple tandem mass spectrometry. Toxicon 2003, 41, 277-283.
13. Ito, E.; Satake, M.; Ofuji, K.; Higashi, M.; Harigaya, K.; McMahon, T.; Yasumoto, T. Chronic effects in mice caused by oral administration of sublethal doses of azaspiracid, a new marine toxin isolated from mussels. Toxicon 2002, 40, 193-203.
14. Vale, P.; Sampayo, M.A.M.; Quilham, M.A. DSP complex toxin profiles relation with Dinophysis spp. occurrence and domoic acid confirmation by LC-MS in Portuguese bivalves. In: Harmful Algae; Reguera, B., Blanco, J., Fernandez, M.L., Wyatt, T., Eds.; Xunta de Galicia and IOC of UNESCO: Spain, 1998; pp. 503-506.
15. Ofuji, K.; Satake, M.; McMahon, T.; Silke, J.; James, K.J.; Naoki, L.; Oshima, Y.; Yasumoto, T. Two analogs of azaspiracid isolated from mussels Mytilus edulis, involved in human intoxication in Ireland. Nat. Toxins 1999, 7, 99-102
16. Ofuji, K.; Satake, M.; Oshima, Y.; McMahon, T.; Silke, J.; James, K.J.; Naoki, L.; Yasumoto, T. A sensitive and specific method for azaspiracids by liquid chromatography mass spectrometry. Nat. Toxins 1999, 7, 247-250.