Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B

Pesquisa Agropecuaria Brasileira

Volumn 43, Issue 10, 2008, Pages 1405-1411

  Radaelli, Paula ^a   Fajardo, Thor Vinícius Martins ^b   Nickel, Osmar ^b   Eiras, Marcelo ^c   Pio Ribeiro, Gilvan ^a  

^a UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO   (Brazil)

^b EMBRAPA UVA E VINHO   (Brazil)

^c INSTITUTO BIOLÓGICO   (Brazil)

Author keywords

GLRaV 2; GVB; Indirect ELISA; Recombinant protein; Vitis; Western blot

Indexed keywords

ESCHERICHIA COLI; GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 2; GRAPEVINE VIRUS B; ORYCTOLAGUS CUNICULUS; VITACEAE; VITIS; VITIVIRUS;

EID: 56849102495     PISSN: 0100204X     EISSN: 16783921     Source Type: Journal    
DOI: 10.1590/S0100-204X2008001000020     Document Type: Article

References (28)

1. ABOU GHANEM-SABANADZOVIC, N.; SABANADZOVIC, S.; CASTELLANO, M.A.; BOSCIA, D.; MARTELLI, G.P. Properties of a new isolate of Grapevine leafroll-associated virus 2. Vitis, v.39, p.119-121, 2000.
2. ABOU-GHANEM, N.; SABANADZOVIC, S.; MINAFRA, A.; SALDARELLI, P.; MARTELLI, G.P. Some properties of Grapevine leafroll-associated virus 2 and molecular organization of the 3′ region of the viral genome. Journal of Plant Pathology, v.80, p.37-46, 1998.
3. 2 fragments of immunoglobulin. Journal of General Virology, v.58, p.315-322, 1982.
4. BERTAZZON, N.; ANGELINI, E. Advances in the detection of Grapevine leafroll-associated virus 2 variants. Journal of Plant Pathology, v.86, p.283-290, 2004.
5. BEUVE, M.; SEMPÉ, L.; LEMAIRE, O. A sensitive one-step real-time RT-PCR method for detecting Grapevine leafroll-associated virus 2 variants in grapevine. Journal of Virological Methods, v.141, p.117-124, 2007.
6. BOSCIA, D.; SAVINO, V.; MINAFRA, A.; NAMBA, S.; ELICIO, V.; CASTELLANO, M.A.; GONSALVES, D.; MARTELLI, G.P. Properties of a filamentous virus isolated from grapevines affected by corky bark. Archives of Virology, v.130, p.109-120, 1993.
7. CLARK, M.F. Immunosorbent assays in plant pathology. Annual Review of Phytopathology, v.19, p.83-106, 1981.
8. FAJARDO, T.V.M.; BARROS, D.R.; NICKEL, O.; KUHN, G.B.; ZERBINI, F.M. Expression of Grapevine leafroll-associated virus 3 coat protein gene in Escherichia coli and production of polyclonal antibodies. Fitopatologia Brasileira, v.32, p.496-500, 2007.
9. FAJARDO, T.V.M.; KUHN, G.B.; NICKEL, O. Doenças virais. In: FAJARDO, T.V.M. (Ed.). Uva para processamento: fitossanidade. Brasília: Embrapa Informação Tecnológica, 2003. p.45-62. (Embrapa Informação Tecnológica. Frutas do Brasil, 35).
10. KOENIG, R. Indirect ELISA methods for the broad specificity detection of plant viruses. Journal of General Virology, v.55, p.53-62, 1981.
11. KOMAR, V.; EMMANUELLE, V.; DEMANGEAT, G.; FUCHS, M. Beneficial effect of selective virus elimination on the performance of Vitis vinifera cv. Chardonnay. American Journal of Enology and Viticulture, v.58, p.202-210, 2007.
12. KUHN, G.B. Intumescimento dos ramos da videira ("corky bark"), doença constatada no Rio Grande do Sul. Fitopatologia Brasileira, v.17, p.399-406, 1992.
13. KUNIYUKI, H.; COSTA, A.S. Estudos sobre a virose fendilhamento cortical da videira em São Paulo. Fitopatologia Brasileira, v.7, p.71-78, 1982.
14. KUNIYUKI, H.; REZENDE, J.A.M.; SCAGLIUSI, S.M.M.; VEGA, J.; YUKI, V.A. Incidência de Grapevine leafroll-associated virus 1, 2 e 3 em vinhedos do Estado de São Paulo. Summa Phytopathologica, v.28, p.311-314, 2002.
15. LING, K.S.; ZHU, H.Y.; JIANG, Z.Y.; GONSALVES, D. Effective application of DAS-ELISA for detection of grapevine leafroll associated closterovirus-3 using a polyclonal antiserum developed from recombinant coat protein. European Journal of Plant Pathology, v.106, p.301-309, 2000.
16. LING, K.S.; ZHU, H.Y.; PETROVIC, N.; GONSALVES, D. Serological detection of Grapevine leafroll virus 2 using an antiserum developed against the recombinant coat protein. Journal of Phytopathology, v.155, p.65-69, 2007.
17. MARTELLI, G.P.; ADAMS, M.J.; KREUZE, J.F.; DOLJA, V.V. Family Flexiviridae: a case study in virion and genome plasticity. Annual Review of Phytopathology, v.45, p.73-100, 2007.
18. MENG, B.; LIN, C.; GOSZCZYNSKI, D.E.; GONSALVES, D. Genome sequences and structures of two biologically distinct strains of Grapevine leafroll-associated virus 2 and sequence analysis. Virus Genes, v.31, p.31-41, 2005.
19. MINAFRA, A.; CASATI, P.; ELICIO, V.; ROWHANI, A.; SALDARELLI, P.; SAVINO, V.; MARTELLI, G.P. Serological detection of Grapevine rupestris stem pitting-associated virus (GRSPaV) by a polyclonal antiserum to recombinant virus coat protein. Vitis, v.39, p.115-118, 2000.
20. MINAFRA, A.; SALDARELLI, P.; GRIECO, F.; MARTELLI, G.P. Nucleotide sequence of the 3' terminal region of the RNA of two filamentous grapevine viruses. Archives of Virology, v.137, p.249-261, 1994.
21. NICKEL, O.; FAJARDO, T.V.M.; ARAGÃO, F.J.L.; CHAGAS, C.M.; KUHN, G.B. Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil. Fitopatologia Brasileira, v.27, p.279-284, 2002.
22. NOUEIRY, A.O.; LUCAS, W.J.; GILBERTSON, R.L. Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell, v.76, p.925-932, 1994.
23. SALDARELLI, P.; MINAFRA, A. Immunodetection of the 20 kDa protein encoded by ORF 2 of Grapevine virus B. Journal of Plant Pathology, v.82, p.157-158, 2000.
24. SALDARELLI, P.; MINAFRA, A.; MARTELLI, G.P. The nucleotide sequence and genomic organization of grapevine virus B. Journal of General Virology, v.77, p.2645-2652, 1996.
25. rd ed. New York: CSHL Press, 2001. 999p.
26. SHI, B.J.; HABILI, N.; GAFNY, R.; SYMONS, R.H. Extensive variation of sequence within isolates of Grapevine virus B. Virus Genes, v.29, p.279-285, 2004.
27. UYEMOTO, J.K.; KRAG, C.R.; ROWHANI, A. An improved purification procedure for grapevine leafroll associated viruses. American Journal of Enology and Viticulture, v.48, p.521-524, 1997.
28. XU, Z.Y.; HONG, N.; XING, B.; WANG, G.P. Partial molecular characterization of a Chinese isolate of Grapevine leafroll-associated virus 2 and production of antisera to recombinant viral proteins. Journal of Plant Pathology, v.88, p.89-94, 2006.