Serological detection of Grapevine rupestris stem pitting-associated virus (GRSPaV) by a polyclonal antiserum to recombinant virus coat protein

Vitis

Volumn 39, Issue 3, 2000, Pages 115-118

  Minafra, A ^a   Casati, P ^a   Elicio, V ^a   Rowhani, A ^a   Saldarelli, P ^a   Savino, V ^a   Martelli, G P ^a  

^a UNIVERSITY OF BARI   (Italy)

Author keywords

Diagnosis; Grapevine rupestris stem pitting associated virus; Recombinant coat protein; Serology; Western blot

Indexed keywords

ANALYTICAL PROCEDURES AND TECHNIQUES; ANTISERUM; CELL MEMBRANE; COAT PROTEIN GENE; POLYVINYL DIFLUORIDE; RABBIT; RECOMBINANT GENE; SERODIAGNOSIS; STEM PITTING ASSOCIATED VIRUS; VIRUS DETECTION;

ESCHERICHIA COLI; VITIS RUPESTRIS;

EID: 0033776294     PISSN: 00427500     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Characteristics of enzyme-linked immunosorbent assay for the detection of the microplate method of plant viruses
2. Rupestris stem pitting
3. An improved procedure for the purification of protein fused with glutathione S-transferase
4. Localization of fruit tree viruses by immuno-tissue printing in infected shoots of Malus sp. and Prunus sp.
5. Rugose wood complex
6. Foveavirus, a new plant virus genus
7. Rupestris stem pitting associated virus-1 is consistently detected in grapevines that are infected with RSP
8. Nucleotide sequence and genome structure of grapevine rupestris stem pitting associated virus-1 reveal similarities to apple stem pitting virus
9. Rupestris stem pitting associated virus-1 consists of a family of sequence variants
10. A chemiluminescent immunoassay for the diagnosis of grapevine closteroviruses on nitrocellulose membrane
11. Serological detection of grapevine virus A using antiserum to a non structural protein, the putative movement protein
12. Nucleotide sequence and RT-PCR detection of a virus associated with grapevine rupestris stem-pitting disease