The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices

Biomaterials

Volumn 30, Issue 4, 2009, Pages 535-540

  Katopodi, Theoni ^a   Tew, Simon R ^b   Clegg, Peter D ^b   Hardingham, Timothy E ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Cartilage; Cell culture; Chondrocyte; ECM; Hyaluronan

Indexed keywords

ANIMAL CELL CULTURE; BIOLOGICAL MATERIALS; BODY FLUIDS; CELL CULTURE; GENE EXPRESSION; GENES; LIGAMENTS; NUCLEIC ACIDS; ORGANIC ACIDS; OXYGEN; PROTEINS; TRANSCRIPTION; TRANSCRIPTION FACTORS;

ARTICULAR CARTILAGE REPAIRS; CARTILAGE DEFECTS; CARTILAGE MATRIX PROTEINS; CARTILAGE MATRIXES; CARTILAGE TISSUES; CHONDROCYTE; CHONDROCYTES; CHONDROGENIC POTENTIALS; ECM; GENES ENCODING; GLYCOSAMINOGLYCAN; HUMAN ARTICULAR CHONDROCYTES; HUMAN CHONDROCYTES; HYALOGRAFT; HYALURONAN; HYPOXIC CONDITIONS; MONOLAYER CULTURES; PROMISING APPROACHES; STANDARD CONDITIONS; TYPE II; WEIGHT RATIOS;

CARTILAGE;

COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN POLYSULFATE;

ARTICLE; BIODEGRADABILITY; CARTILAGE CELL; CARTILAGE MATRIX; CELL CULTURE; COLLAGEN SYNTHESIS; CONTROLLED STUDY; DONOR; GENE EXPRESSION; GRAFT PERFUSION; HUMAN; HUMAN CELL; HYPOXIA; OSTEOARTHRITIS; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

BIOCOMPATIBLE MATERIALS; CARTILAGE, ARTICULAR; CELL HYPOXIA; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; GLYCOSAMINOGLYCANS; HUMANS; OSTEOARTHRITIS; TISSUE DONORS;

EID: 56449120478     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2008.09.064     Document Type: Article

References (30)

1. Hardingham T., Tew S., and Murdoch A. Tissue engineering: chondrocytes and cartilage. Arthritis Res 4 Suppl. 3 (2002) S63-S68
2. Archer C.W., and Francis-West P. The chondrocyte. Int J Biochem Cell Biol 35 4 (2003) 401-404
3. Brittberg M., Lindahl A., Nilsson A., Ohlsson C., Isaksson O., and Peterson L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331 14 (1994) 889-895
4. Freed L.E., Marquis J.C., Nohria A., Emmanual J., Mikos A.G., and Langer R. Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 27 1 (1993) 11-23
5. Grigolo B., Lisignoli G., Piacentini A., Fiorini M., Gobbi P., Mazzotti G., et al. Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAff 11): molecular, immunohistochemical and ultrastructural analysis. Biomaterials 23 4 (2002) 1187-1195
6. Solchaga L.A., Dennis J.E., Goldberg V.M., and Caplan A.I. Hyaluronic acid-based polymers as cell carriers for tissue-engineered repair of bone and cartilage. J Orthop Res 17 2 (1999) 205-213
7. Laurent T.C., and Fraser J.R. Hyaluronan. FASEB J 6 7 (1992) 2397-2404
8. von der Mark K., Gauss V., von der Mark H., and Muller P. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 267 5611 (1977) 531-532
9. Benya P.D., and Shaffer J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30 1 (1982) 215-224
10. Kuettner K.E., Pauli B.U., Gall G., Memoli V.A., and Schenk R.K. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphology. J Cell Biol 93 3 (1982) 743-750
11. Grimshaw M.J., and Mason R.M. Modulation of bovine articular chondrocyte gene expression in vitro by oxygen tension. Osteoarthr Cartil 9 4 (2001) 357-364
12. Zhou S., Cui Z., and Urban J.P. Factors influencing the oxygen concentration gradient from the synovial surface of articular cartilage to the cartilage-bone interface: a modeling study. Arthritis Rheum 50 12 (2004) 3915-3924
13. Marcacci M., Berruto M., Brocchetta D., Delcogliano A., Ghinelli D., Gobbi A., et al. Articular cartilage engineering with Hyalograft C: 3-year clinical results. Clin Orthop Relat Res 435 (2005) 96-105
14. Nehrer S., Domayer S., Dorotka R., Schatz K., Bindreiter U., and Kotz R. Three-year clinical outcome after chondrocyte transplantation using a hyaluronan matrix for cartilage repair. Eur J Radiol 57 1 (2006) 3-8
15. Tew S.R., Li Y., Pothacharoen P., Tweats L.M., Hawkins R.E., and Hardingham T.E. Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthr Cartil 13 1 (2005) 80-89
16. Farndale R.W., Sayers C.A., and Barrett A.J. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res 9 4 (1982) 247-248
17. Martin I., Jakob M., Schafer D., Dick W., Spagnoli G., and Heberer M. Quantitative analysis of gene expression in human articular cartilage from normal and osteoarthritic joints. Osteoarthr Cartil 9 2 (2001) 112-118
18. Li Y., Tew S.R., Russell A.M., Gonzalez K., Hardingham T.E., and Hawkins R.E. Transduction of human articular chondrocytes with adenoviral, retroviral and lentiviral vectors and the effects of enhanced expression of SOX9. Tissue Eng 10 3/4 (2004) 575-584
19. Adesida A.B., Grady L.M., Khan W.S., and Hardingham T.E. The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia. Arthritis Res Ther 8 3 (2006) R61
20. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25 4 (2001) 402-408
21. Wardale R.J., and Duance V.C. Quantification and immunolocalisation of porcine articular and growth plate cartilage collagens. J Cell Sci 105 Pt 4 (1993) 975-984
22. Domm C., Schunke M., Christesen K., and Kurz B. Redifferentiation of dedifferentiated bovine articular chondrocytes in alginate culture under low oxygen tension. Osteoarthr Cartil 10 1 (2002) 13-22
23. Hansen U., Schunke M., Domm C., Ioannidis N., Hassenpflug J., Gehrke T., et al. Combination of reduced oxygen tension and intermittent hydrostatic pressure: a useful tool in articular cartilage tissue engineering. J Biomech 34 7 (2001) 941-949
24. Murphy C.L., and Sambanis A. Effect of oxygen tension on chondrocyte extracellular matrix accumulation. Connect Tissue Res 42 2 (2001) 87-96
25. Kurz B., Domm C., Jin M., Sellckau R., and Schunke M. Tissue engineering of articular cartilage under the influence of collagen I/III membranes and low oxygen tension. Tissue Eng 10 7-8 (2004) 1277-1286
26. Lafont J.E., Talma S., and Murphy C.L. Hypoxia-inducible factor 2alpha is essential for hypoxic induction of the human articular chondrocyte phenotype. Arthritis Rheum 56 10 (2007) 3297-3306
27. Khan W.S., Adesida A.B., and Hardingham T.E. Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients. Arthritis Res Ther 9 3 (2007) R55
28. Adesida A.B., Grady L.M., Khan W.S., Millward-Sadler S.J., Salter D.M., and Hardingham T.E. Human meniscus cells express hypoxia inducible factor-1alpha and increased SOX9 in response to low oxygen tension in cell aggregate culture. Arthritis Res Ther 9 4 (2007) R69
29. Lafont J.E., Talma S., Hopfgarten C., and Murphy C.L. Hypoxia promotes the differentiated human articular chondrocyte phenotype through SOX9-dependent and -independent pathways. J Biol Chem 283 8 (2008) 4778-4786
30. Brew C.J., Andrew J.G., Boot-Handford R., and Hardingham T.E. Increased collagen I expression in osteoarthritic cartilage suggests some loss of normal chondrocyte phenotype. Trans Orthop Res Soc 50 (2004) 940