Determination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection

Sensors

Volumn 8, Issue 11, 2008, Pages 7097-7112

  Gazdik, Zbynek ^a,b,c   Zitka, Ondrej ^c,d   Petrlova, Jitka ^c,d   Adam, Vojtech ^b,c,d   Zehnalek, Josef ^c,d   Horna, Ales ^e   Reznicek, Vojtech ^a,b,c   Beklova, Miroslava ^f   Kizek, Rene ^c,d  

^a Faculty of Horticulture   (Czech Republic)

^b Faculty of Agronomy   (Czech Republic)

^c MENDEL UNIVERSITY IN BRNO   (Czech Republic)

^d MASARYK UNIVERSITY   (Czech Republic)

^e TOMAS BATA UNIVERSITY IN ZLIN   (Czech Republic)

^f UNIVERSITY OF VETERINARY AND PHARMACEUTICAL SCIENCES   (Czech Republic)

Author keywords

Ascorbic acid; Electrochemical detection; Flow injection analysis; Fruits; High performance liquid chromatography; Human blood serum; Pharmaceutical preparation

Indexed keywords

AMPEROMETRIC DETECTOR; ELECTROCHEMICAL DETECTION; ELECTROCHEMICAL DETECTORS; EXPERIMENTAL CONDITIONS; FLOW INJECTION ANALYSIS; HUMAN BLOODS; PHARMACEUTICAL PREPARATIONS; WATER SOLUBLE ORGANIC COMPOUNDS;

BLOOD; CALIBRATION; CHROMATOGRAPHY; COULOMETERS; DETECTORS; ELECTROCHEMICAL SENSORS; FRUITS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OPTIMIZATION;

ASCORBIC ACID;

EID: 56449105282     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s8117097     Document Type: Article

References (72)

1. Velisek, J.; Cejpek, K. Biosynthesis of food constituents: Vitamins. 2. Water-soluble vitamins: part 1 - a review. Czech. J. Food Sci. 2007, 25, 49-64.
2. Linster, C.L.; Van Schaftingen, E. Vitamin C - Biosynthesis, recycling and degradation in mammals. Febs J. 2007, 274, 1-22.
3. Davey, M.W.; Van Montagu, M.; Inze, D.; Sanmartin, M.; Kanellis, A.; Smirnoff, N.; Benzie, I.J.J.; Strain, J.J.; Favell, D.; Fletcher, J. Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J. Sci. Food Agric. 2000, 80, 825-860.
4. Noctor, G.; Foyer, C.H. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Molec. Biol. 1998, 49, 249-279.
5. Kleszczewska, E. L-Ascorbic acid - clinical use, toxicity, properties, methods of determination and application in chemical analysis. Pharmazie 2000, 55, 640-644.
6. Meister, A. Glutathione Ascorbic-Acid Antioxidant System in Animals. J. Biol. Chem. 1994, 269, 9397-9400.
7. Englard, S.; Seifter, S. The Biochemical Functions of Ascorbic-Acid. Annu. Rev. Nutr. 1986, 6, 365-406.
8. Levine, M.; Rumsey, S.C.; Daruwala, R.; Park, J.B.; Wang, Y.H. Criteria and recommendations for vitamin C intake. JAMA-J. Am. Med. Assoc. 1999, 281, 1415-1423.
9. Gomez-Romero, M.; Arraez-Roman, D.; Segura-Carretero, A.; Fernandez-Gutierrez, A. Analytical determination of antioxidants in tomato: Typical components of the Mediterranean diet. J. Sep. Sci. 2007, 30, 452-461.
10. Deicher, R.; Horl, W.H. Vitamin C in chronic kidney disease and hemodialysis patients. Kidney Blood Pressure Res. 2003, 26, 100-106.
11. Ogiri, Y.; Sun, F.; Hayami, S.; Fujimura, A.; Yamamoto, K.; Yaita, M.; Kojo, S. Very low vitamin C activity of orally administered L-dehydroascorbic acid. J. Agric. Food Chem. 2002, 50, 227-229.
12. Prasad, B.B.; Tiwari, K.; Singh, M.; Sharma, P.S.; Patel, A.K.; Srivastava, S. Molecularly imprinted polymer-based solid-phase microextraction fiber coupled with molecularly imprinted polymer-based sensor for ultratrace analysis of ascorbic acid. J. Chromatogr. A 2008, 1198, 59-66.
13. Karlsen, A.; Blomhoff, R.; Gundersen, T.E. Stability of whole blood and plasma ascorbic acid. Eur. J Clin. Nutr. 2007, 61, 1233-1236.
14. Yao, X.; Wang, Y.T.; Chen, G. Simultaneous determination of am aminothiols, ascorbic acid and uric acid in biological samples by capillary electrophoresis with electrochemical detection. Biomed. Chromatogr. 2007, 27, 520-526.
15. Koide, K.; Zhang, X.M.; Ohishi, K.; Usami, Y.; Hotta, Y.; Hiramitsu, T. Ascorbic acid concentration in rabbit vitreous measured by microdialysis with HPLC-electrochemical detection before and after vitreous surgery. Exp. Eye Res. 2006, 82, 868-873.
16. Cofan, C.; Radovan, C. Simultaneous chronoamperometric sensing of ascorbic acid and acetaminophen at a boron-doped diamond electrode. Sensors 2008, 8, 3952-3969.
17. Wang, Y.; Xu, H.; Zhang, J.M.; Li, G. Electrochemical sensors for clinic analysis. Sensors 2008, 8, 2043-2081.
18. Yogeswaran, U.; Chen, S.M. A review on the electrochemical sensors and biosensors composed of nanowires as sensing material. Sensors 2008, 5, 290-313.
19. Chen, J.; Fang, Y.J. Flow injection technique for biochemical analysis with chemiluminescence detection in acidic media. Sensors 2007, 7, 448-458.
20. Shaidarova, L.G.; Gedmina, A.V.; Chelnokova, I.A.; Budnikov, G.K. Electrocatalytic oxidation and flow-injection determination of ascorbic acid at a graphite electrode modified with a polyaniline film containing electrodeposited palladium. J. Anal. Chem. 2006, 61, 601-608.
21. Matos, R.C.; Augelli, M.A.; Lago, C.L.; Angnes, L. Flow injection analysis-amperometric determination of ascorbic and uric acids in urine using arrays of gold microelectrodes modified by electrodeposition of palladium. Anal. Chim. Acta 2000, 404, 151-157.
22. Janda, P.; Weber, J.; Dunsch, L.; Lever, A.B.P. Detection of ascorbic acid using a carbon fiber microelectrode coated with cobalt tetramethylpyridoporphyrazine. Anal. Chem. 1996, 68, 960-965.
23. Behrens, W.A.; Madere, R. A Highly Sensitive High-Performance Liquid-Chromatography Method for the Estimation of Ascorbic and Dehydroascorbic Acid in Tissues, Biological-Fluids, and Foods. Anal. Biochem. 1987, 165, 102-107.
24. Shakya, R.; Navarre, D.A. Rapid screening of ascorbic acid, glycoalkaloids, and phenolics in potato using high-performance liquid chromatography. J. Agric. Food Chem. 2006, 54, 5253-5260.
25. Melendez-Martinez, A.J.; Vicario, I.M.; Heredia, F.J. Provitamin A carotenoids and ascorbic acid contents of the different types of orange juices marketed in Spain. Food Chem. 2007, 101, 177-184.
26. Wang, J.; Chatrathi, M.P.; Tian, B.M.; Polsky, R. Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen. Anal. Chem. 2000, 72, 2514-2518.
27. Davey, M.W.; Bauw, G.; VanMontagu, M. Simultaneous high-performance capillary electrophoresis analysis of the reduced and oxidised forms of ascorbate and glutathione. J. Chromatogr. B 1997, 697, 269-276.
28. Klejdus, B.; Petrlova, J.; Potesil, D.; Adam, V.; Mikelova, R.; Vacek, J.; Kizek, R.; Kuban, V. Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Anal. Chim. Acta 2004, 520, 57-67.
29. Wu, T.; Guan, Y.Q.; Ye, J.N. Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection. Food Chem. 2007, 100, 1573-1579.
30. Sun, X.M.; Niu, Y.; Bi, S.; Zhang, S.S. Determination of ascorbic acid in individual rat hepatocyte by capillary electrophoresis with electrochemical detection. J. Chromatogr. B 2008, 570, 46-50.
31. Zuo, F.; Luo, C.H.; Zheng, Z.H.; Ding, X.B.; Peng, Y.X. Supramolecular assembly of beta-cyclodextrin-capped gold nanoparticles on ferrocene- functionalized ITO surface for enhanced voltammetric analysis of ascorbic acid. Electroanalysis 2008, 20, 894-899.
32. Razmi, H.; Harasi, M. Voltammetric behavior and amperometric determination of ascorbic acid at cadmium pentacyanonitrosylferrate film modified GC electrode. Int. J. Electrochem. Sci. 2008, 3, 82-95.
33. Abbaspour, A.; Khajehzadeh, A.; Noori, A. A simple and selective sensor for the determination of ascorbic acid in vitamin C tablets based on paptode. Anal. Sci. 2008, 24, 721-725.
34. Liu, H.Q.; Tian, Y. Analytical application of pyramidal, rodlike, and spherical gold nanostructures: Simultaneous detection of ascorbic acid and uric acid. Electroanalysis 2008, 20, 1227-1233.
35. Dai, H.; Wu, X.P.; Wang, Y.M.; Zhou, W.C.; Chen, G.N. An electrochemiluminescent biosensor for vitamin C based on inhibition of luminol electrochemiluminescence on graphite/poly(methyl methacrylate) composite electrode. Electrochim. Acta 2008, 53, 5113-5117.
36. Nezamzadeh, A.; Amini, M.K.; Faghihian, H. Square-wave voltammetric determination of ascorbic acid based on its electrocatalytic oxidation at zeolite-modified carbon-paste electrodes. Int. J. Electrochem. Sci. 2007, 2, 583-594.
37. Kizek, R.; Vacek, J.; Trnkova, L.; Jelen, F. Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine. Bioelectrochemistry 2004, 63, 19-24.
38. Mikelova, R.; Baloun, J.; Petrlova, J.; Adam, V.; Havel, L.; Petrek, J.; Horna, A.; Kizek, R. Electrochemical determination of Ag-ions in environment waters and their action on plant embryos. Bioelectrochemistry 2007, 70, 508-518.
39. Causon, R. Validation of chromatographic methods in biomedical analysis - Viewpoint and discussion. J. Chromatogr. B 1997, 689, 175-180.
40. Bugianesi, R.; Serafini, M.; Simone, F.; Wu, D.Y.; Meydani, S.; Ferro-Luzzi, A.; Azzini, E.; Maiani, G. High-performance liquid chromatography with coulometric electrode array detector for the determination of quercetin levels in cells of the immune system. Anal. Biochem. 2000, 284, 296-300.
41. Petrlova, J.; Mikelova, R.; Stejskal, K.; Kleckerova, A.; Zitka, O.; Petrek, J.; Havel, L.; Zehnalek, J.; Adam, V.; Trnkova, L.; Kizek, R. Simultaneous determination of eight biologically active thiol compounds using gradient elution-liquid chromatography with Coul-Array detection. J. Sep. Sci. 2006, 29, 1166-1173.
42. Long, G.L.; Winefordner, J.D. Limit of Detection. Anal. Chem. 1983, 55, A712-A724.
43. Masarik, M.; Stobiecka, A.; Kizek, R.; Jelen, F.; Pechan, Z.; Hoyer, W.; Jovin, T.M.; Subramaniam, V.; Palecek, E. Sensitive electrochemical detection of native and aggregated alpha-synuclein protein involved in Parkinson's disease. Electroanalysis 2004, 16, 1172-1181.
44. Kizek, R.; Trnkova, L.; Palecek, E. Determination of metallothionein at the femtomole level by constant current stripping chronopotentiometry. Anal. Chem. 2001, 73, 4801-4807.
45. Hubalek, J.; Hradecky, J.; Adam, V.; Kryštofova, O.; Huska, D.; Masarik, M.; Trnkova, L.; Horna, A.; Klosova, K.; Adamek, M.; Zehnalek, J.; Kizek, R. Spectrometric and voltammetric analysis of urease - Nickel nanoelectrode as an electrochemical sensor. Sensors 2007, 7, 1238-1255.
46. Petrlova, J.; Krizkova, S.; Supalkova, V.; Masarik, M.; Adam, V.; Havel, L.; Kramer, K.J.; Kizek, R. The determination of avidin in genetically modified maize by voltammetric techniques. Plant Soil Environ. 2007, 53, 345-349.
47. Petrlova, J.; Krizkova, S.; Zitka, O.; Hubalek, J.; Prusa, R.; Adam, V.; Wang, J.; Beklova, M.; Sures, B.; Kizek, R. Utilizing a chronopotentiometric sensor technique for metallothionein determination in fish tissues and their host parasites. Sens. Actuator B-Chem. 2007, 127, 112-119.
48. Petrlova, J.; Masarik, M.; Potesil, D.; Adam, V.; Trnkova, L.; Kizek, R. Zeptomole detection of streptavidin using carbon paste electrode and square wave voltammetry. Electroanalysis 2007, 19, 1177-1182.
49. Fabrik, I.; Krizkova, S.; Huska, D.; Adam, V.; Hubalek, J.; Trnkova, L.; Eckschlager, T.; Kukacka, J.; Prusa, R.; Kizek, R. Employment of electrochemical techniques for metallothionein determination in tumour cell lines and patients with a tumour disease. Electroanalysis 2008, 20, 1521-1532.
50. Fabrik, I.; Ruferova, Z.; Hilscherova, K.; Adam, V.; Trnkova, L.; Kizek, R. A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction. Sensors 2008, 5, 4081-4094.
51. Adam, V.; Baloun, J.; Fabrik, I.; Trnkova, L.; Kizek, R. An electrochemical detection of metallothioneins in nanolitres at zeptomole level. Sensors 2008, 5, 2293-2305.
52. Adam, V.; Blastik, O.; Krizkova, S.; Lubal, P.; Kukacka, J.; Prusa, R.; Kizek, R. Application of the Brdicka reaction in determination of metallothionein in patients with tumours. Chem. Listy 2008, 102, 51-58.
53. Adam, V.; Zitka, O.; Dolezal, P.; Zeman, L.; Horna, A.; Hubalek, J.; Sileny, J.; Krizkova, S.; Trnkova, L.; Kizek, R. Lactoferrin isolation using monolithic column coupled with spectrometric or micro-amperometric detector. Sensors 2008, 5, 464-487.
54. Zitka, O.; Stejskal, K.; Kleckerova, A.; Adam, V.; Beklova, M.; Horna, A.; Supalkova, V.; Havel, L.; Kizek, R. Utilizing electrochemical techniques for detection of biological samples. Chem. Listy 2001, 101, 225-231.
55. Kand'ar, R.; Zakova, P.; Lotkova, H.; Kucera, O.; Cervinkova, Z. Determination of reduced and oxidized glutathione in biological samples using liquid chromatography with fluorimetric detection. J. Pharm. Biomed. Anal. 2007, 43, 1382-1387.
56. Supalkova, V.; Huska, D.; Diopan, V.; Hanustiak, P.; Zitka, O.; Stejskal, K.; Baloun, J.; Pikula, J.; Havel, L.; Zehnalek, J.; Adam, V.; Trnkova, L.; Beklova, M.; Kizek, R. Electroanalysis of plant thiols. Sensors 2007, 7, 932-959.
57. Supalkova, V.; Petrek, J.; Baloun, J.; Adam, V.; Bartusek, K.; Trnkova, L.; Beklova, M.; Diopan, V.; Havel, L.; Kizek, R. Multi-instrumental investigation of affecting of early somatic embryos of Spruce by cadmium(II) and lead(II) ions. Sensors 2007, 7, 743-759.
58. Supalkova, V.; Beklova, M.; Baloun, J.; Singer, C.; Sures, B.; Adam, V.; Huska, D.; Pikula, J.; Rauscherova, L.; Havel, L.; Zehnalek, J.; Kizek, R. Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry. Bioelectrochemistry 2008, 72, 59-65.
59. Krizkova, S.; Ryant, P.; Krystofova, O.; Adam, V.; Galiova, M.; Beklova, M.; Babula, P.; Kaiser, J.; Novotny, K.; Novotny, J.; Liska, M.; Malina, R.; Zehnalek, J.; Hubalek, J.; Havel, L.; Kizek, R. Multi-instrumental analysis of tissues of sunflower plants treated with silver(I) ions - Plants as bioindicators of environmental pollution. Sensors 2008, 5, 445-463.
60. Ryant, P.; Dolezelova, E.; Fabrik, I.; Baloun, J.; Adam, V.; Babula, P.; Kizek, R. Electrochemical determination of low molecular mass thiols content in potatoes (Solanum tuberosum) cultivated in the presence of various sulphur forms and infected by late blight (Phytophora infestans). Sensors 2008, 5, 3165-3182.
61. Babula, P.; Huska, D.; Hanustiak, P.; Baloun, J.; Krizkova, S.; Adam, V.; Hubalek, J.; Havel, L.; Zemlicka, M.; Horna, A.; Beklova, M.; Kizek, R. Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples. Sensors 2006, 6, 1466-1482.
62. Supalkova, V.; Stavelikova, H.; Krizkova, S.; Adam, V.; Horna, A.; Havel, L.; Ryant, P.; Babula, P.; Kizek, R. Study of capsaicin content in various parts of pepper fruit by liquid chromatography with electrochemical detection. Acta Chim. Slov. 2007, 54, 55-59.
63. Kolouchova-Hanzlikova, I.; Melzoch, K.; Filip, V.; Smidrkal, J. Rapid method for resveratrol determination by HPLC with electrochemical and UV detections in wines. Food Chem. 2004, 57, 151-158.
64. Diopan, V.; Babula, P.; Shestivska, V.; Adam, V.; Zemlicka, M.; Dvorska, M.; Hubalek, J.; Trnkova, L.; Havel, L.; Kizek, R. Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide. J. Pharm. Biomed. Anal. 2008, 48, 127-133.
65. Supalkova, V.; Petrek, J.; Havel, L.; Krizkova, S.; Petrlova, J.; Adam, V.; Potesil, D.; Babula, P.; Beklova, M.; Horna, A.; Kizek, R. Electrochemical sensors for detection of acetylsalicylic acid. Sensors 2006, 6, 1483-1497.
66. Mikelova, R.; Hodek, P.; Hanustiak, P.; Adam, V.; Krizkova, S.; Havel, L.; Stiborova, M.; Horna, A.; Beklova, M.; Trnkova, L.; Kizek, R. Determination of isoflavones using liquid chromatography with electrochemical detection. Acta Chim. Slov. 2007, 54, 92-97.
67. Billova, S.; Kizek, R.; Jelen, F.; Novotna, P. Square-wave voltammetric determination of cefoperazone in a bacterial culture, pharmaceutical drug, milk, and urine. Anal. Bioanal. Chem. 2003, 377, 362-369.
68. Diculescu, V.C.; Vivan, M.; Brett, A.M.O. Voltammetric behavior of antileukemia drug glivec. Part III: In situ DNA oxidative damage by the Glivec electrochemical metabolite. Electroanalysis 2006, 18, 1963-1970.
69. Beklova, M.; Krizkova, S.; Supalkova, V.; Mikelova, R.; Adam, V.; Pikula, J.; Kizek, R. Determination of bromadiolone in pheasants and foxes by differential pulse voltammetry. Int. J. Environ. Anal. Chem. 2007, 57, 459-469.
70. Krizkova, S.; Beklova, M.; Pikula, J.; Adam, V.; Horna, A.; Kizek, R. Hazards of secondary bromadiolone intoxications evaluated using high-performance liquid chromatography with electrochemical detection. Sensors 2007, 7, 1271-1286.
71. Potesil, D.; Petrlova, J.; Adam, V.; Vacek, J.; Klejdus, B.; Zehnalek, J.; Trnkova, L.; Havel, L.; Kizek, R. Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection. J. Chromatogr. A 2005, 1084, 134-144.
72. Wang, J. Analytical Electrochemistry, Wiley-VCH: New York, 2000.