Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

Journal of Biotechnology

Volumn 134, Issue 1-2, 2008, Pages 127-136

  Vidal, Luis ^a   Pinsach, Jaume ^a   Striedner, Gerald ^c   Caminal, Glòria ^b   Ferrer, Pau ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b DEPARTMENT OF ENVIRONMENTAL CHEMISTRY   (Spain)

^c UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

Antibiotic free plasmid selection system; Escherichia coli; Glycine auxotrophy complementation; Recombinant protein production; Rhamnulose 1 phosphate aldolase

Indexed keywords

ANTIBIOTICS; BIOMARKERS; DNA; GENE EXPRESSION; GROWTH KINETICS; PROTEINS;

ANTIBIOTIC-FREE PLASMID SELECTION SYSTEMS; GLYCINE AUXOTROPHY COMPLEMENTATION; RECOMBINANT PROTEIN PRODUCTION;

ESCHERICHIA COLI;

AMINO ACID; GLYCINE; GLYCINE HYDROXYMETHYLTRANSFERASE; PLASMID VECTOR; RECOMBINANT PROTEIN;

AMINO ACID SYNTHESIS; ARTICLE; AUXOTROPHY; BACTERIAL GROWTH; BIOSYNTHESIS; CELL DENSITY; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION SYSTEM; GROWTH RATE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

ALDEHYDE-LYASES; ANTI-BACTERIAL AGENTS; ESCHERICHIA COLI; GLYCINE; MODELS, GENETIC; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 39749143172     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.01.011     Document Type: Article

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