A TaqMan® reverse transcription polymerase chain reaction (RT-PCR) in vitro potency assay for plasmid-based vaccine products

Molecular Biotechnology

Volumn 40, Issue 1, 2008, Pages 47-57

  Mahajan, Rohit ^a   Feher, Beth ^a   Jones, Basil ^a   Jones, Doug ^a   Marjerison, Lana ^a   Sam, Mindy ^a   Hartikka, Jukka ^a   Wloch, Mary ^a   Lalor, Peggy ^a   Kaslow, David ^a,b   Hall, Keith ^a   Rolland, Alain ^a  

^a VICAL INC   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

pDNA; Plasmid based vaccines; Potency assay; Real time PCR; RT PCR

Indexed keywords

BODY FLUIDS; DNA; GENES; NUCLEIC ACIDS; ORGANIC ACIDS; POLYMERIZATION; POLYMERS; PUMPS; RNA; TRANSCRIPTION; VACCINES;

PDNA; PLASMID-BASED VACCINES; POTENCY ASSAY; REAL TIME PCR; RT-PCR;

COSMIC RAYS;

DNA VACCINE; PLASMID DNA; UNCLASSIFIED DRUG; VCL CB01 VACCINE; PRIMER DNA; VACCINE;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG POTENCY; GENETIC TRANSFECTION; IMMUNE RESPONSE; IN VITRO STUDY; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; ANIMAL; CELL CULTURE; GENETICS; HUMAN; IMMUNOLOGY; METHODOLOGY; MOUSE; NUCLEOTIDE SEQUENCE; PLASMID; SENSITIVITY AND SPECIFICITY;

HUMAN HERPESVIRUS 5; MUS;

ANIMALS; BASE SEQUENCE; CELLS, CULTURED; DNA PRIMERS; HUMANS; MICE; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VACCINES;

EID: 55949113518     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9058-3     Document Type: Article

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