Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR

Veterinary Microbiology

Volumn 110, Issue 3-4, 2005, Pages 167-179

  Dalla Valle, L ^a   Toffolo, V ^a   Lamprecht, M ^a   Maltese, C ^b   Bovo, G ^b   Belvedere, P ^a   Colombo, L ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE VENEZIE   (Italy)

Author keywords

Betanodavirus; Diagnostic assay; Encephalopathy and retinopathy; Real time PCR; Viral nervous necrosis

Indexed keywords

COAT PROTEIN; DYE; PRIMER RNA; RNA DIRECTED RNA POLYMERASE; SYBR GREEN I; UNCLASSIFIED DRUG; VIRUS RNA;

AGAR GEL ELECTROPHORESIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BETANODAVIRUS; BRAIN NECROSIS; CELL CULTURE; CLADISTICS; CONTROLLED STUDY; CORRELATION ANALYSIS; CROSS REACTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; FISH; FISH DISEASE; GENE AMPLIFICATION; GENE TARGETING; GENOME ANALYSIS; JUVENILE; LARVA; MELTING POINT; MORTALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE DIAGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; REGRESSION ANALYSIS; RELIABILITY; RNA VIRUS; SENSITIVITY AND SPECIFICITY; TELEOST; VARIANCE; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS GENOME; VIRUS INFECTION; VIRUS ISOLATION;

ANIMALS; CENTRAL NERVOUS SYSTEM INFECTIONS; FISH DISEASES; FISHES; NODAVIRIDAE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA VIRUS INFECTIONS; RNA VIRUSES; RNA, RIBOSOMAL, 18S; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL PROTEINS;

BETANODAVIRUS; RNA VIRUSES; STRIPED JACK NERVOUS NECROSIS VIRUS; TELEOSTEI;

EID: 25444496523     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2005.07.014     Document Type: Article

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