A colorimetric assay for viral agents that produce cytopathic effects

Journal of Virological Methods

Volumn 135, Issue 1, 2006, Pages 56-65

  Heldt, Caryn L ^a   Hernandez, Raquel ^b   Mudiganti, Usharani ^b   Gurgel, Patrick V ^c   Brown, Dennis T ^b   Carbonell, Ruben G ^a  

^a North Carolina State University   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

^c ProMetic Life Sciences Inc   (Canada)

Author keywords

MTT; Parvovirus; Plaque assay; Sindbis virus; TCID50

Indexed keywords

ANTIVIRUS AGENT; TETRAZOLIUM;

ANIMAL CELL; ARTICLE; COLORIMETRY; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOPATHOGENIC EFFECT; NONHUMAN; PORCINE PARVOVIRUS; PRIORITY JOURNAL; SINDBIS VIRUS; VIRUS INFECTION; VIRUS INFECTIVITY; VIRUS PLAQUE; VIRUS TITRATION;

ANIMALS; CELL LINE; COLORIMETRY; CRICETINAE; CYTOPATHOGENIC EFFECT, VIRAL; DOGS; FORMAZANS; HUMANS; MINUTE VIRUS OF MICE; PARVOVIRUS, CANINE; PARVOVIRUS, PORCINE; PLAQUE ASSAY; REPRODUCIBILITY OF RESULTS; SINDBIS VIRUS; SWINE; TETRAZOLIUM SALTS; VIRUSES;

ANIMALIA; CANINE PARVOVIRUS; MINUTE VIRUS OF MICE; PARVOVIRUS; PORCINE PARVOVIRUS; SINDBIS VIRUS;

EID: 33646562842     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.01.022     Document Type: Article

References (29)

1. Andersson P., Alm S., Edman K., and Lindberg A.M. A novel and rapid method to quantify cytolytic replication of picornaviruses in cell culture. J. Virol. Methods 130 (2005) 117-123
2. Ayisi N.K., Gupta S.V., and Qualtiere L.F. Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds. J. Virol. Methods 33 (1991) 335-344
3. Baba M., Snoeck R., Pauwels R., and de Clercq E. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob. Agents Chemother. 32 (1988) 1742-1745
4. Baylis S.A., Shah N., and Minor P.D. Evaluation of different assays for detection of parvovirus B19 DNA in human plasma. J. Virol. Methods 121 (2004) 7-16
5. Chang S.F., Sgro J.Y., and Parrish C.R. Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. J. Virol. 66 (1992) 6858-6867
6. Hernandez R., Lee H., Nelson C., and Brown D.T. A single deletion in the membrane-proximal region of the Sindbis virus glycoprotein E2 endodomain blocks virus assembly. J. Virol. 74 (2000) 4220-4228
7. Huang C., Hung J.J., Wu C.Y., and Chien M.S. Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. Vet. Microbiol. 101 (2004) 209-214
8. Ifrah M., Stienlauf S., Shoresh M., and Katz E. A tetrazolium-based colorimetric assay for titration of neutralizing antibodies against vaccinia virus. Viral Immunol. 11 (1998) 49-54
9. Kawamura H., Fujita T., and Imada T. Plaque formation and replication of porcine parvovirus in embryonic swine kidney cell line, ESK cells. Jpn. J. Vet. Sci. 50 (1988) 803-808
10. Kimura K., Mori S., Tomita K., Ohno K., Takahashi K., Shigeta S., and Terada M. Antiviral activity of NMSO3 against respiratory syncytial virus infection in vitro and in vivo. Antiviral Res. 47 (2000) 41-51
11. Levi R., Beeor-Tzahar T., and Arnon R. Microculture virus titration-a simple colourimetric assay for influenza virus titration. J. Virol. Methods 52 (1995) 55-64
12. Miekka S.I., Busby T.F., Reid B., Pollock R., Ralston A., and Drohan W.N. New methods for inactivation of lipid-enveloped and non-enveloped viruses. Haemophilia 4 (1998) 402-408
13. Molitor T.W., Joo H.S., and Collett M.S. Porcine parvovirus: virus purification and structural and antigentic properties of virion polypeptides. J. Virol. 45 (1983) 842-854
14. Molitor T.W., Oraveerakul K., Zhang Q.Q., Choi C.S., and Ludemann L.R. Polymerase chain reaction (PCR) amplification for the detection of porcine parvovirus. J. Virol. Methods 32 (1991) 201-211
15. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 (1983) 55-63
16. Muzyczka N., and Berns K.I. Parvoviridae: the viruses and their replication. In: Knipe D.M., and Howely P.M. (Eds). Field's Virology Vol. 2 (2001), Lippincott-Raven 2327-2359
17. Oraveerakul K., Choi C.S., and Molitor T.W. Restriction of porcine parvovirus replication in nonpermissive cells. J. Virol. 66 (1992) 715-722
18. Park R., Giza P.E., Mold D.E., and Huang R.C.C. Inhibition of HSV-1 replication and reactiviation by the mutation-insensitive transcription inhibitor tetra-O-glycyl-nordihydroguaiaretic acid. Antiviral Res. 58 (2003) 35-45
19. Payment P., and Trudel M. Methods and Techniques in Virology (1993), Marcel Dekkel
20. Prikhod'ko G.G., Reyes H., Vasilyeva I., and Busby T.F. Establishment of a porcine parvovirus (PPV) DNA standard and evaluation of a new LightCycler nested-PCR assay for detection of PPV. J. Virol. Methods 111 (2003) 13-19
21. Prikhod'ko G.G., Vasilyeva I., Reyes H., Wong S., Brown K.E., Jameson T., and Busby T.F. Evaluation of a new LightCycler reverse transcription-polymerase chain reaction infectivity assay for detection of human parvovirus B19 in dry-heat inactivation studies. Transfusion 45 (2005) 1011-1019
22. Renz D., and Brown D.T. Characteristics of Sindbis virus temperature-sensitive mutants in cultured BHK-21 and Aedes albopictus (mosquito) cells. J. Virol. 19 (1976) 775-781
23. Rubino K.L., and Nicholas J.A. A novel, spectrophotometric microneutralization assay for respiratory syncytial virus. J. Virol. Methods 39 (1992) 55-67
24. Soares R.M., Durigon E.L., Bersano J.G., and Richtzenhain L.J. Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1. J. Virol. Methods 78 (1999) 191-198
25. In: Spearman A.K.G., and Cavalli-Sforza L. (Eds). Biometrie. Grundzuge Biologisch-medizinischer Statistik (1974), Gustav Fisher, Stuttgart
26. Sudo K., Konno K., Yokota T., and Shigeta S. A sensitive assay system screening antiviral compounds against herpes simplex virus type 1 and type 2. J. Virol. Methods 49 (1994) 169-178
27. Takeuchi H., Baba M., and Shigeta S. An application of tetrazolium (MTT) colorimetric assay for the screening of anti-herpes simplex virus compounds. J. Virol. Methods 33 (1991) 61-71
28. Tattersall P., and Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J. Virol. 46 (1983) 944-955
29. Wickramasinghe S.R., Han B., Carlson J.O., and Powers S.M. Clearance of minute virus of mice by flocculation and microfiltration. Biotechnol. Bioeng. 86 (2004) 612-621