Exploring the intramolecular phosphorylation sites in human Chk2

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 646, Issue 1-2, 2008, Pages 50-59

  Olsen, Birgitte B ^a   Larsen, Martin R ^a   Boldyreff, Brigitte ^b   Niefind, Karsten ^c   Issinger, Olaf Georg ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b KinaseDetect ApS   (Denmark)

^c UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Auto activation; Chk2; Mass spectrometric analysis; Phosphorylation; Site directed mutagenesis

Indexed keywords

CHECKPOINT KINASE 2; PROTEIN P53;

ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; DNA DAMAGE; ENZYME ACTIVITY; FIBROBLAST; GEL FILTRATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CELL CYCLE PROTEINS; CELL LINE; DNA-BINDING PROTEINS; ENZYME ACTIVATION; ESCHERICHIA COLI; FIBROBLASTS; HUMANS; KIDNEY; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION; TUMOR SUPPRESSOR PROTEINS;

HEXAPODA;

EID: 55749083186     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2008.09.002     Document Type: Article

References (30)

1. Bartek J., Bartkova J., and Lukas J. DNA damage signaling guards against activated oncogenes and tumor progression. Oncogene 26 (2007) 7773-7779
2. Ahn J.Y., Schwartz J.K., Piwnica-Worms H., and Canman C.E. Threonine 68 phosphorylation by ataxia telangiectasia mutated is required for efficient activation of Chk2 in response to ionizing radiation. Cancer Res. 60 (2000) 5934-5936
3. Matsuoka S., Rotman G., Ogawa A., Shiloh Y., Tamia K., and Elledge S.J. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. Proc. Natl. Acad. Sci. U.S.A. 97 (2000) 10389-10394
4. Melchionna R., Chen X.B., Blasina A., and McGowan C.H. Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1. Nat. Cell Biol. 2 (2000) 762-765
5. Ahn J., and Prives C. Checkpoint kinase 2 (Chk2) monomers or dimers phosphorylated Cdc25C after DNA damage regardless of threonine 68 phosphorylation. J. Biol. Chem. 277 (2002) 48418-48426
6. Schwarz J.K., Lovly C.M., and Piwnica-Worms H. Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation. Mol. Cancer Res. 1 (2003) 598-609
7. Lee C.H., and Chung J.H. The hCds1 (Chk2)-FHA domain is essential for a chain of phosphorylation events on hCds1 that is induced by ionizing radiation. J. Biol. Chem. 276 (2001) 30537-30541
8. Wu X., and Chen J. Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis. J. Biol. Chem. 278 (2003) 36163-36168
9. Oliver A.W., Paul A., Boxall K.J., Barrie S.E., Aherne G.W., Garrett M.D., Mittnacht S., and Pearl L.H. Trans-activation of the DNA-damage signaling protein kianse Chk2 by T-loop exchange. EMBO J. 25 (2006) 3179-3190
10. Buscemi G., Carlessi L., Zannini L., Lisanti S., Fontanella E., Canevari S., and Delia D. DNA damage-induced cell cycle regulation and function of novel Chk2 phosphoresidues. Mol. Cell. Biol. 26 (2006) 7832-7845
11. Bahassi El.M., Myer D.L., McKenney R.J., Hennigan R.F., and Stambrook P.J. Priming phopshorylation of Chk2 by polo-like kinase 3 (Plk3) mediates its full activation by ATM and a downstream checkpoint in response to DNA damage. Mutat. Res. 596 (2006) 166-176
12. Kass E.M., Ahn J., Tanaka T., Freed-Pastor W.A., Keezer S., and Prives C. Stability of checkpoint kinase 2 is regulated via phosphorylation at serine 456. J. Biol. Chem. 282 (2007) 30311-30319
13. King J.B., Gross J., Lovly C.M., Rohrs H., Piwnica-Worms H., and Townsend R.R. Accurate mass-driven analysis for the characterization of protein phosphorylation. Study of the human Chk2 protein kinase. Anal. Chem. 78 (2006) 2171-2181
14. King J.B., Gross J., Lovly C.M., Piwnica-Worms H., and Townsend R.R. Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadropole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun. Mass Spectrom. 21 (2007) 3443-3451
15. Bartek J., and Lukas J. Chk1 and Chk2 kinases in checkpoint control and cancer. Cancer Cell 3 (2003) 421-429
16. Craig A., Scott M., Burch L., Smith G., Ball K., and Hupp T. Allosteric effects mediate CHK2 phosphorylation of the p53 transactivation domain. EMBO Rep. 4 (2003) 787-792
17. Olsen B.B., Niefind K., and Issinger O.G. Inter- and Supramolecular interactions of protein kinase CK2 and their relevance for genome integrity. In: Lankenau D.H. (Ed). Genome Dyn. Stab (1), Genome integrity (2005), Springer, Berlin 315-342
18. Bjørling-Poulsen M., Siehler S., Wiesmüller L., Meek D., Niefind K., and Issinger O.G. The "regulatory" beta-subunit of protein kinase CK2 negatively influences p53-mediated allosteric effects on Chk2 activation. Oncogene 24 (2005) 6194-6200
19. Larsen M.R., Thingholm T.E., Jensen O.N., Roepstorff P., and Jørgensen T.D. Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol. Cell. Proteomics 4 (2005) 873-886
20. Jensen S.S., and Larsen M.R. Evaluation of phosphopeptide enrichment procedures. Rapid. Commun. Mass Spectrom. 21 (2007) 3635-3645
21. Thingholm T.E., Jørgensen T.J.D., Jensen O.N., and Larsen M.R. Highly selective enrichment of phosphorylated peptides using titanium dioxide. Nat. Protoc. 1 (2006) 1929-1935
22. Schroeder M.J., Shabanowitz J., Schwartz J.C., Hunt D.F., and Coon J.J. A neutral loss activation method for improved phosphopeptide sequence analysis by quadropole ion trap mass spectrometry. Anal. Chem. 76 (2004) 3590-3598
23. Povirk L.F. DNA damage and mutagenesis by radiomimetic DNA-cleaving agents: bleomycin, neocarzinostatin and other enediynes. Mut. Res. 355 (1996) 71-89
24. Toledo F., and Wahl G.M. Regulating the p53 pathway; in vitro hypotheses, in vivo veritas. Nat. Rev. Cancer 6 (2006) 909-923
25. Li J., and Stern D.F. Regulation of Chk2 by DNA-dependent protein kinase. J. Biol. Chem. 280 (2005) 12041-12050
26. Pabla N., Huang S., Mi Q.S., Daniel R., and Dong Z. ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis. J. Biol. Chem. 283 (2008) 6572-6583
27. Wei J.H., Chou Y.F., Ou Y.H., Yeh Y.H., Tyan S.W., Sun T.P., Shen C.Y., and Shieh S.Y. TTK/hMps1 participates in the regulation of DNA damage checkpoint response by phosphorylating Chk2 on threonine 68. J. Biol. Chem. 280 (2005) 7748-7757
28. Li J., Williams B.L., Haire L.F., Goldberg M., Wilker E., Durocher D., Yaffe M.B., Jackson S.P., and Smerdon S.J. Structural and functional versatility of the FHA domain in DNA damage signaling by the tumor suppressor kinase Chk2. Mol. Cell 9 (2002) 1045-1054
29. Abraham R.T. Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes Dev. 15 (2001) 2177-2196
30. Gupta S.K., Guo X., Durkin S.S., Fryrear K.F., Ward M.D., and Semmes O.J. Human T-cell leukemia virus type 1 Tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated Chk2. J. Biol. Chem. 282 (2007) 29431-29430