Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold

FASEB Journal

Volumn 22, Issue 11, 2008, Pages 3795-3804

  Fan, Chia Yu ^a   Huang, Chuan Chuan ^a   Chiu, Wei Chun ^a   Lai, Chun Chieh ^b   Liou, Gunn Guang ^b   Li, Hsiu Chuan ^a   Chou, Min Yuan ^a  

^a INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

^b NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

Author keywords

CD3; EGFR; Phage display; Single chain antibody

Indexed keywords

ANTIBODY CONJUGATE; ANTIBODY CONJUGATE ERB_SCFV-COL; BINDING PROTEIN; COLLAGEN; CYSTEINE; EPIDERMAL GROWTH FACTOR RECEPTOR; GLYCYLPROLYLPROLINE; IMMUNOGLOBULIN G; SCAFFOLD PROTEIN; SECRETORY PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BLOOD SAMPLING; CIRCULAR DICHROISM; DISULFIDE BOND; DRUG BLOOD LEVEL; ELECTRON MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; HYDROXYLATION; MALE; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SURFACE PLASMON RESONANCE; THERMOSTABILITY;

ANIMALS; CELL LINE, TUMOR; COLLAGEN; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MICE; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS;

MAMMALIA;

EID: 55549103706     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fj.08-111484     Document Type: Article

References (31)

1. Riechmann, L., Clark, M., Waldmann, H., and Winter, G. (1988) Reshaping human antibodies for therapy. Nature 332, 323-327
2. Queen, C., Schneider, W. P., Selick, H. E., Payne, P. W., Landolfi, N. F., Duncan, J. F., Avdalovic, N. M., Levitt, M., Junghans, R. P., and Waldmann, T. A. (1989) A humanized antibody that binds to the interleukin 2 receptor. Proc. Natl. Acad. Sci. U. S. A. 86, 10029-10033
3. Dubel, S., Breitling, F., Kontermann, R., Schmidt, T., Skerra, A., and Little, M. (1995) Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). J. Immunol. Methods 178, 201-209
4. Rheinnecker, M., Hardt, C., Ilag, L. L., Kufer, P., Gruber, R., Hoess, A., Lupas, A., Rottenberger, C., Pluckthun, A., and Pack, P. (1996) Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. J. Immunol. 157, 2989-2997
5. Binz, H. K., Amstutz, P., Kohl, A., Stumpp, M. T., Briand, C., Forrer, P., Grutter, M. G., and Pluckthun, A. (2004) High-affinity binders selected from designed ankyrin repeat protein libraries. Nat. Biotechnol. 22, 575-582
6. Silverman, J., Liu, Q., Bakker, A., To, W., Duguay, A., Alba, B. M., Smith, R., Rivas, A., Li, P., Le, H., Whitehorn, E., Moore, K. W., Swimmer, C., Perlroth, V., Vogt, M., Kolkman, J., and Stemmer, W. P. (2005) Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains. Nat. Biotechnol. 23, 1556-1561
7. Graversen, J. H., Jacobsen, C., Sigurskjold, B. W., Lorentsen, R. H., Moestrup, S. K., Thogersen, H. C., and Etzerodt, M. (2000) Mutational analysis of affinity and selectivity of kringle-tetranectin interaction. Grafting novel kringle affinity onto the tetranectin lectin scaffold. J. Biol. Chem. 275, 37390-37396
8. Berg, R. A., and Prockop, D. J. (1973) The thermal transition of a non-hydroxylated form of collagen. Evidence for a role for hydroxyproline in stabilizing the triple-helix of collagen. Biochem. Biophys. Res. Commun. 52, 115-120
9. Rosenbloom, J., Harsch, M., and Jimenez, S. (1973) Hydroxyproline content determines the denaturation temperature of chick tendon collagen. Arch. Biochem. Biophys. 158, 478-484
10. Chopra, R. K., and Ananthanarayanan, V. S. (1982) Conformational implications of enzymatic proline hydroxylation in collagen. Proc. Natl. Acad. Sci. U. S. A. 79, 7180-7184
11. Yang, W., Chan, V. C., Kirkpatrick, A., Ramshaw, J. A., and Brodsky, B. (1997) Gly-Pro-Arg confers stability similar to Gly-Pro-Hyp in the collagen triple-helix of host-guest peptides. J. Biol. Chem. 272, 28837-28840
12. De Wildt, R. M., Mundy, C. R., Gorick, B. D., and Tomlinson, I. M. (2000) Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat. Biotechnol. 18, 989-994
13. Arakawa, F., Kuroki, M., Kuwahara, M., Senba, T., Ozaki, H., Matsuoka, Y., Misumi, Y., Kanda, H., and Watanabe, T. (1996) Cloning and sequencing of the VH and V kappa genes of an anti-CD3 monoclonal antibody, and construction of a mouse/human chimeric antibody. J. Biochem. (Tokyo) 120, 657-662
14. Cole, M. S., Anasetti, C., and Tso, J. Y. (1997) Human IgG2 variants of chimeric anti-CD3 are nonmitogenic to T cells. J. Immunol. 159, 3613-3621
15. Liou, G. G., Tanny, J. C., Kruger, R. G., Walz, T., and Moazed, D. (2005) Assembly of the SIR complex and its regulation by O-acetyl-ADP-ribose, a product of NAD-dependent histone deacetylation. Cell 121, 515-527
16. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
17. Adair, J. R., Athwal, D. S., Bodmer, M. W., Bright, S. M., Collins, A. M., Pulito, V. L., Rao, P. E., Reedman, R., Rothermel, A. L., and Xu, D. (1994) Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum. Antibodies Hybridomas 5, 41-47
18. Frank, S., Kammerer, R. A., Mechling, D., Schulthess, T., Landwehr, R., Bann, J., Guo, Y., Lustig, A., Bachinger, H. P., and Engel, J. (2001) Stabilization of short collagen-like triple helices by protein engineering. J. Mol. Biol. 308, 1081-1089
19. Holler, N., Tardivel, A., Kovacsovics-Bankowski, M., Hertig, S., Gaide, O., Martinon, F., Tinel, A., Deperthes, D., Calderara, S., Schulthess, T., Engel, J., Schneider, P., and Tschopp, J. (2003) Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex. Mol. Cell. Biol. 23, 1428-1440
20. Hoppe, H. J., Barlow, P. N., and Reid, K. B. (1994) A parallel three stranded alpha-helical bundle at the nucleation site of collagen triple-helix formation. FEBS Lett. 344, 191-195
21. Bachinger, H. P., Bruckner, P., Timpl, R., Prockop, D. J., and Engel, J. (1980) Folding mechanism of the triple helix in type-III collagen and type-III pN-collagen. Role of disulfide bridges and peptide bond isomerization. Eur. J. Biochem. 106, 619-632
22. Prockop, D. J., and Kivirikko, K. I. (1995) Collagens: molecular biology, diseases, and potentials for therapy. Annu. Rev. Biochem. 64, 403-434
23. Engel, J., Chen, H. T., Prockop, D. J., and Klump, H. (1977) The triple helix in equilibrium with coil conversion of collagen-like polytripeptides in aqueous and nonaqueous solvents. Comparison of the thermodynamic parameters and the binding of water to (L-Pro-L-Pro-Gly)n and (L-Pro-L-Hyp-Gly)n. Biopolymers 16, 601-622
24. Boudko, S., Frank, S., Kammerer, R. A., Stetefeld, J., Schulthess, T., Landwehr, R., Lustig, A., Bachinger, H. P., and Engel, J. (2002) Nucleation and propagation of the collagen triple helix in single-chain and trimerized peptides: transition from third to first order kinetics. J. Mol. Biol. 317, 459-470
25. Frank, S., Boudko, S., Mizuno, K., Schulthess, T., Engel, J., and Bachinger, H. P. (2003) Collagen triple helix formation can be nucleated at either end. J. Biol. Chem. 278, 7747-7750
26. Li, H. C., Huang, C. C., Chen, S. F., and Chou, M. Y. (2005) Assembly of homotrimeric type XXI minicollagen by coexpression of prolyl 4-hydroxylase in stably transfected Drosophila melanogaster S2 cells. Biochem. Biophys. Res. Commun. 336, 375-385
27. Chou, M. Y., and Li, H. C. (2002) Genomic organization and characterization of the human type XXI collagen (COL21A1) gene. Genomics 79, 395-401
28. Mazzorana, M., Cogne, S., Goldschmidt, D., and Aubert-Foucher, E. (2001) Collagenous sequence governs the trimeric assembly of collagen XII. J. Biol. Chem. 276, 27989-27998
29. Li, J., Davis, J., Bracht, M., Carton, J., Armstrong, J., Gao, W., Scallon, B., Fung, R., Emmell, E., Zimmerman, M., Griswold, D. E., and Li, L. (2006) Modulation of antigen-specific T cell response by a non-mitogenic anti-CD3 antibody. Int. Immunopharmacol. 6, 880-891
30. Kabat, E. A., Wu, T. T., Perry, H. M., Gottesman, K. S., and Foeller, C. (1991) Sequences of Proteins of Immunological Interest, National Institutes of Health, Bethesda, Maryland, USA
31. Kivirikko, K. I., Myllyla, R., and Pihlajaniemi, T. (1992) Hydroxylation of proline and lysine residues in collagens and other animal and plant proteins. In Post-Translational Modifications of Proteins (Harding, J. J., and Crabbe, M. J. C., eds) pp. 1-51, CRC Press, Boca Raton, FL, USA