An in vitro protocol for recording from spinal motoneurons of adult rats

Journal of Neurophysiology

Volumn 100, Issue 1, 2008, Pages 474-481

  Carp, Jonathan S ^a,b   Tennissen, Ann M ^a   Mongeluzi, Donna L ^a   Dudek, Christopher J ^a   Xiang, Yang Chen ^a   Wolpaw, Jonathan R ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

^b WADSWORTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KETAMINE; MACROGOL; XYLAZINE; CHOLERA TOXIN; MACROGOL DERIVATIVE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BRAIN FUNCTION; BRAIN SLICE; CELL POPULATION; CONTROLLED STUDY; IN VITRO STUDY; LAMINECTOMY; MALE; MOTONEURON; NONHUMAN; PRIORITY JOURNAL; RAT; SPINAL CORD; SPINAL CORD NERVE CELL; ANIMAL; CELL MEMBRANE POTENTIAL; CYTOLOGY; ELECTROPHYSIOLOGY; INNERVATION; LIMB; METABOLISM; METHODOLOGY; PATCH CLAMP; PHYSIOLOGY; TRANSPLANTATION;

ANIMALS; CHOLERA TOXIN; ELECTROPHYSIOLOGY; EXTREMITIES; MALE; MEMBRANE POTENTIALS; MOTOR NEURONS; PATCH-CLAMP TECHNIQUES; POLYETHYLENE GLYCOLS; RATS; SPINAL CORD; TISSUE AND ORGAN HARVESTING;

EID: 55249114848     PISSN: 00223077     EISSN: 15221598     Source Type: Journal    
DOI: 10.1152/jn.90422.2008     Document Type: Article

References (53)

1. Acker T, Acker H. Cellular oxygen sensing need in CNS function: physiological and pathological implications. J Exp Biol 207: 3171-3188, 2004.
2. Albensi BC. The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Curr Pharm Des 13: 3185-3194, 2007.
3. Bagust J, Kerkut GA. An in vitro preparation of the spinal cord of the mouse. In: Electrophysiology of Isolated Mammalian CNS Preparations, edited by Kerkut GA, Wheal HV. London: Academic Press, 1981, p. 337-365.
4. Beaumont E, Gardiner PF. Endurance training alters the biophysical properties of hindlimb motoneurons in rats. Muscle Nerve 27: 228-236, 2003.
5. Bennett DJ, Li Y, Siu M. Plateau potentials in sacrocaudal motoneurons of chronic spinal rats, recorded in vitro. J Neurophysiol 86: 1955-1971, 2001.
6. Berger AJ. Recent advances in respiratory neurobiology using in vitro methods. Am J Physiol Lung Cell Mol Physiol 259: L24-L29, 1990.
7. Biscoe TJ, Duchen MR. Synaptic physiology of spinal motoneurones of normal and spastic mice: an in vitro study. J Physiol 379: 275-292, 1986.
8. Brock LG, Coombs JS, Eccles JC. Action potentials of motoneurons with intracellular electrode. Proc Univ Otago Med Sch 29: 14-15, 1951.
9. Brownstone R. Beginning at the end: repetitive firing properties in the final common pathway. Prog Neurobiol 78: 156-172, 2006.
10. Burke RE. Motor units: anatomy, physiology and functional organization. In: Handbook of Physiology. The Nervous System. Motor Control. Bethesda, MD: Am. Physiol. Soc., 1981, sect. 1, vol. II, pt. 1, p. 345-422.
11. Burke RE. John Eccles' pioneering role in understanding central synaptic transmission. Prog Neurobiol 78: 173-188, 2006.
12. Button D, Gardiner K, Marqueste T, Gardiner P. Frequency-current relationships of rat hindlimb alpha-motoneurones. J Physiol 573: 663-677, 2006.
13. Carlin KP, Jiang Z, Brownstone RM. Characterization of calcium currents in functionally mature mouse spinal motoneurons. Eur J Neurosci 12: 1624-1634, 2000.
14. Carp JS. Physiological properties of primate lumbar motoneurons. J Neurophysiol 68: 1121-1132, 1992.
15. Carp JS, Mongeluzi DL, Tennissen AM, Chen XY, Wolpaw JR. Intracellular recordings from lumbar spinal motoneurons of adult rats and mice in vitro. Program No. 404.8. 2007 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2007. Online.
16. Carp JS, Tennissen AM, Wolpaw JR. Intracellular recording from adult mouse spinal motoneurons in vitro: methods development. Program No. 496.15. 2003 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2003. Online.
17. Carriedo SG, Yin HZ, Weiss JH. Motor neurons are selectively vulnerable to AMPA/kainate receptor-mediated injury in vitro. J Neurosci 16: 4069-4079, 1996.
18. Chen XY, Wolpaw JR. Triceps surae motoneuron morphology in the rat: a quantitative light microscopic study. J Comp Neurol 343: 143-157, 1994.
19. Collins WF 3rd, Seymour A, Klugewicz S. Differential effect of castration on the somal size of pudendal motoneurons in the adult male rat. Brain Res 577: 326-330, 1992.
20. Cormery B, Beaumont E, Csukly K, Gardiner P. Hindlimb unweighting for 2 weeks alters physiological properties of rat hindlimb motoneurones. J Physiol 568: 841-850, 2005.
21. Davies ML, Kirov SA, Andrew RD. Whole isolated neocortical and hippocampal preparations and their use in imaging studies. J Neurosci Methods 166: 203-216, 2007.
22. Dingledine R, Dodd J, Kelly J. The in vitro brain slice as a useful neurophysiological preparation for intracellular recording. J Neurosci Methods 2: 323-362, 1980.
23. Duggal N, Lach B. Selective vulnerability of the lumbosacral spinal cord after cardiac arrest and hypotension. Stroke 33: 116-121, 2002.
24. Dunwiddie T, Mueller A, Basile A. The use of brain slices in central nervous system pharmacology. Fed Proc 42: 2891-2898, 1983.
25. Fulton BP. Motoneurone activity in an isolated spinal cord preparation from the adult mouse. Neurosci Lett 71: 175-180, 1986.
26. Gardiner PF. Physiological properties of motoneurons innervating different muscle unit types in rat gastrocnemius. J Neurophysiol 69: 1160-1170, 1993.
27. Gustafsson B, Pinter MJ. An investigation of threshold properties among cat spinal alpha-motoneurones. J Physiol 357: 453-483, 1984.
28. Harvey PJ, Li Y, Li X, Bennett DJ. Persistent sodium currents and repetitive firing in motoneurons of the sacrocaudal spinal cord of adult rats. J Neurophysiol 96: 1141-1157, 2006.
29. Heckmann CJ, Gorassini MA, Bennett DJ. Persistent inward currents in motoneuron dendrites: implications for motor output. Muscle Nerve 31: 135-156, 2005.
30. Hirayama T, Ono H, Fukuda H. Unit activity of ventral horn cells in spinal cord slices isolated from adult rats. Neurosci Lett 91: 343-348, 1988.
31. Hochman S, Fedirchuk B, Shefchyk S. Membrane electrical properties of external urethral and external anal sphincter somatic motoneurons in the decerebrate cat. Neurosci Lett 127: 87-90, 1991.
32. Hori N, Tan Y, King M, Strominger N, Carpenter D. Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices. Brain Res 958: 434-438, 2002.
33. Hori N, Tan Y, Strominger N, Carpenter D. Intracellular activity of rat spinal cord motoneurons in slices. J Neurosci Methods 112: 185-191, 2001.
34. Jiang Z, Rempel J, Li J, Sawchuk MA, Carlin KP, Brownstone RM. Development of L-type calcium channels and a nifedipine-sensitive motor activity in the postnatal mouse spinal cord. Eur J Neurosci 11: 3481-3487, 1999.
35. Kerkut GA, Bagust J. The isolated mammalian spinal cord. Prog Neurobiol 46: 1-48, 1995.
36. Krnjevic K. Early effects of hypoxia on brain cell function. Croat Med J 40: 375-380, 1999.
37. Lentz B. PEG as a tool to gain insight into membrane fusion. Eur Biophys J 36: 315-326, 2007.
38. Li Y, Bennett DJ. Persistent sodium and calcium currents cause plateau potentials in motoneurons of chronic spinal rats. J Neurophysiol 90: 857-869, 2003.
39. Lore AB, Hubbell JA, Bobb DS Jr, Ballinger ML, Loftin KL, Smith JW, Smyers ME, Garcia HD, Bittner GD. Rapid induction of functional and morphological continuity between severed ends of mammalian or earthworm myelinated axons. J Neurosci 19: 2442-2454, 1999.
40. McKenna K, Nadelhaft I. The organization of the pudendal nerve in the male and female rat. J Comp Neurol 248: 532-549, 1986.
41. Nicolopoulos-Stournaras S, Iles JF. Motor neuron columns in the lumbar spinal cord of the rat. J Comp Neurol 217: 75-85, 1983.
42. Nohda K, Nakatsuka T, Takeda D, Miyazaki N, Nishi H, Sonobe H, Yoshida M. Selective vulnerability to ischemia in the rat spinal cord: a comparison between ventral and dorsal horn neurons. Spine 32: 1060-1066, 2007.
43. Rekling JC, Funk GD, Bayliss DA, Dong XW, Feldman JL. Synaptic control of motoneuronal excitability. Physiol Rev 80: 767-852, 2000.
44. Sasaki M. Membrane properties of external urethral and external anal sphincter motoneurones in the cat. J Physiol 440: 345-366, 1991.
45. Schroder H. Organization of the motoneurons innervating the pelvic muscles of the male rat. J Comp Neurol 192: 567-587, 1980.
46. Sharp FR, Ran R, Lu A, Tang Y, Strauss KI, Glass T, Ardizzone T, Bernaudin M. Hypoxic preconditioning protects against ischemic brain injury. NeuroRx 1: 26-35, 2004.
47. Shi R, Borgens R. Anatomical repair of nerve membranes in crushed mammalian spinal cord with polyethylene glycol. J Neurocytol 29: 633-643, 2000.
48. Shi R, Borgens R, Blight A. Functional reconnection of severed mammalian spinal cord axons with polyethylene glycol. J Neurotrauma 16: 727-738, 1999.
49. Steiger HJ, Hanggi D. Ischaemic preconditioning of the brain, mechanisms and applications. Acta Neurochir (Wien) 149: 1-10, 2007.
50. Takahashi T. Intracellular recording from visually identified motoneurons in rat spinal cord slices. Proc R Soc Lond B Biol Sci 202: 417-421, 1978.
51. Theiss RD, Heckman CJ. Systematic variation in effects of serotonin and norepinephrine on repetitive firing properties of ventral horn neurons. Neuroscience 134: 803-815, 2005.
52. Ulfhake B, Kellerth JO. A quantitative light microscopic study of the dendrites of cat spinal alpha-motoneurons after intracellular staining with horseradish peroxidase. J Comp Neurol 202: 571-583, 1981.
53. 2+, mitochondria and selective motoneuron vulnerability: implications for ALS. Trends Neurosci 28: 494-500, 2005.