Development of a PCR-denaturing gradient gel electrophoresis method targeting the tuf gene to differentiate and identify Staphylococcus species

Journal of Food and Drug Analysis

Volumn 16, Issue 4, 2008, Pages 44-51

  Chen, Hsin Chih ^a   Hwang, Wen Zhe ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

DGGE; Identification; Staphylococcal poisons; Staphylococcus species; Tuf gene

Indexed keywords

GENE PRODUCT; PROTEIN TUF; STAPHYLOCOCCUS ENTEROTOXIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; CULTURE TECHNIQUE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; FOOD CONTAMINATION; GENE TARGETING; MILK; NONHUMAN; POLYMERASE CHAIN REACTION; SPECIES DIFFERENTIATION; STAPHYLOCOCCUS;

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INTERMEDIUS; STAPHYLOCOCCUS WARNERI;

EID: 55049141829     PISSN: 10219498     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Fueyo, J. M., Martin, M. C., Gonzalez-Hevia, M. A. and Mendoza, M. C. 2001. Enterotoxin production and DNA fingerprinting in Staphylococcus aureus isolated from human and food samples. Relations between genetic types and enterotoxins. Int. J. Food Microbiol. 67: 139-145.
2. Becker, K., Keller, B., Von Eiff, C., Bruck, M., Lubritz, G., Etitenne, J. and Peters, G. 2001. Enterotoxigenic potential of Staphylococcus intermedius. Appl. Environ. Microbiol. 67: 5551-5557.
3. Carmo, L. S., Dias, R. S., Linardi, V. R., De Sena, M. J., Santos, D. A., de Faria, M. E., Pena, E. C., Jett, M. and Heneine, L. G. 2002. Food poisoning due to enterotoxigenic strains of Staphylococcus present in Minas cheese and rawmilk in Brazil. Food Microbiol. 19: 9-14.
4. Chou, C. C. and Chen, L. F. 1997. Enterotoxin production by Staphylococcus warneri CCRC 12929, a coagulase-negative strain. J. Food Prot. 60: 923-927.
5. Futagawa-Saito, K., Suzuki, M., Ohsawa, M., Ohshima, S., Sakurai, N., Ba-Thein, W. and Fukuyasu, T. 2004. Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons. J. Appl. Microbiol. 96: 1361-1366.
6. Vernozy Rozand, C., Mazuy, C., Prevost, G., Lapeyre, C., Bes, M., Brun, Y. and Fleurette, J. 1996. Enterotoxin production by coagulase -negative staphylococci isolated from goats' milk and cheese. Int. J. of Food Microbiol. 30: 271-280.
7. Guardabassi, L., Loeber, M. E. and Jacobson, A. 2004. Transmission of multiple antimicrobial-resistant Staphylococcus intermedius between dogs affected by deep pyoderma and their owners. Vet. Microbiol. 98: 23-27.
8. Zschöck, M., Nesseler, A. and Sudarwanto, I. 2005. Evaluation of six commercial identification kits for the identification of Staphylococcus aureus isolated from bovine mastitis. J. Appl. Microbiol. 98: 450-455.
9. Ieven, M., Verhoeven, J., Pattyn, S. R. and Goossens, H. 1995. Rapid and economical method for species identification of clinically significant coagulase-negative staphylococci. J. Clin. Microbiol. 33: 1060-1063.
10. Poyart, C., Quesne, G., Boumaila, C. and Trieu-Cuot, P. 2001. Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target. J. Clin. Microbiol. 39: 4296-4301.
11. Blaiotta, G., Pennacchia, C., Ercolini, D., Moschetti, G. and Villani, F. 2003. Combining denaturing gradient gel electrophoresis of 16S rDNA V3 Region and 16S-23S rDNA spacer region polymorphism analyses for the identification of staphylococci from Italian fermented sausages. Syst. Appl. Microbiol. 26: 423-433.
12. Chesneau, O., Morvan, A., Aubert, S. and el Solh, N. 2000. The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus. Int. J. Syst. Evol. Microbiol. 50: 689-697.
13. Martineau, F., Picard, F. J., Ke, D., Paradis, S., Roy, P., Ouellette, M. and Bergeron, M. G. 2001. Development of a PCR assay for identification of staphylococci at genus and species levels. J. Clin. Microbiol. 39: 2541-2547.
14. Mason, W. J., Blevins, J. S., Beenken, K., Wibowo, N., Ojha, N. and Smeltze, M. S. 2001. Multiplex PCR protocol for the diagnosis of staphylococcal infection. J. Clin. Microbiol. 39: 3332-3338.
15. Yazdankhah, S. P., Hellemann, A. L., Rønningen, K. and Olsen, E. 1998. Rapid and sensitive detection of Staphylococcus species in milk by ELISA based on monodisperse magnetic particles. Vet. Microbiol. 62: 17-26.
16. Martineau, F., Picard, F. J., Roy, P. H., Ouellette, M. and Bergeron, M. G. 1996. Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis. J. Clin. Microbiol. 34: 2888-2893.
17. Zakrzewska-Czerwinska, J., Gaszewska-Mastalarz, A., Pulverer, G. and Mordarski, M. 1992. Identification of Staphylococcus epidermidis using a 16S rRNA-directed oligonucleotide probe. FEMS Microbiol. Lett. 79: 51-58.
18. Maes, N., De Gheldre, Y., De Ryck, R., Vaneechoutte, M., Meugnier, H., Etifnne, J. and Struelens, M. J. 1997. Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis. J. Clin. Microbiol. 35: 2477-2481.
19. Drancourt, M. and Raoult, D. 2002. rpoB gene sequence-based identification of Staphylococcus species. J. Clin. Microbiol. 40: 1333-1338.
20. Goh, S., Santucci, Z., Kloos, W. E., Faltyn, M., George, C. G., Driedger, D. and Hemmingsen, S. M. 1997. Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization. J. Clin. Microbiol. 35: 3116-3121.
21. Vannuffel, P., Heusterspreute, M., Bouyer, M., Vandercam, B., Philippe, M. and Gala, J. L. 1999. Molecular characterization of femA from Staphylococcus hominis and Staphylococcus saprophyticus, and femA-based discrimination of staphylococcal species. Res. Microbiol. 150: 129-141.
22. Ercolini, D. 2004. PCR-DGGE fingerprinting: Novel strategies for detection of microbes in food. J. Microbiol. Methods 56: 297-314.
23. Rantsiou, K., Iacumin, L., Canton, C., Comi, G. and Cocolin, L. 2005. Ecology and characterization by molecular methods of Staphylococcus species isolated from fresh sausages. Int. J. Food Microbiol. 97: 277-284.
24. Kamath, U., Singer, C. and Isenberg, H. D. 1992. Clinical Significance of Staphylococcus warneri Bacteremia. J. Clin. Microbiol. 30: 261-264.
25. Seifert, H., Oltmanns, D., Becker, K., Wisplinghoff, H. and von Eiff, C. 2005. Staphylococcus lugdunensis Pacemaker-related infection. Emerg. Infect. Dis. 11: 1283-1286.
26. Forsman, P., Tilsala-Timisjarvi, A. and Alatossava, T. 1997. Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions. Microbiology 143: 3491-3500.
27. Couto, I., Pereira, S., Miragala, M., Sanches, I.S. and De Lencastre, H. 2001. Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR. J. Clin. Microbiol. 39: 3099-3103.
28. Chavagnat, F., Haueter, M., Jimeno, J. and Casey, M. G. 2002. Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol. Lett. 217: 177-183.
29. Ventura, M., Canchaya, C., Meylan, V., Klaenhammer, T. R. and Zink, R. 2003. Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification. Appl. and Environ. Microbiol. 69: 6908-6922.