Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability

Stem Cells

Volumn 26, Issue 10, 2008, Pages 2552-2563

  Dick, Emily Patricia ^a   Prince, Lynne Rebecca ^a   Sabroe, Ian ^a,b  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b ROYAL HALLAMSHIRE HOSPITAL   (United Kingdom)

Author keywords

CD34+ progenitors; Differentiation; Ex vivo expansion; Hematopoiesis; Neutrophils

Indexed keywords

CATHELICIDIN ANTIMICROBIAL PEPTIDE LL 37; CATHEPSIN G; CD14 ANTIGEN; CD34 ANTIGEN; ELASTASE; FORMYLMETHIONYLLEUCYLPHENYLALANINE; MYELOPEROXIDASE; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 4;

ARTICLE; BACTERICIDAL ACTIVITY; BLOOD; BONE MARROW; CD34 SELECTION; CELL EXPANSION; CONTROLLED STUDY; DISEASE DURATION; ESCHERICHIA COLI; EX VIVO STUDY; HUMAN; HUMAN CELL; NEUTROPENIA; NEUTROPHIL; PHAGOCYTOSIS; RESPIRATORY BURST; STEM CELL; STREPTOCOCCUS PNEUMONIAE; WESTERN BLOTTING;

ADULT; ANTIGENS, CD34; ANTIMICROBIAL CATIONIC PEPTIDES; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CHEMOTAXIS; CYTOKINES; CYTOPLASMIC GRANULES; ESCHERICHIA COLI; HUMANS; LEUKOCYTE ELASTASE; MICROBIAL VIABILITY; NEUTROPHILS; PHAGOCYTOSIS; RESPIRATORY BURST; STEM CELLS; STREPTOCOCCUS PNEUMONIAE; TOLL-LIKE RECEPTORS;

ESCHERICHIA COLI; STREPTOCOCCUS PNEUMONIAE;

EID: 55049141135     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2008-0328     Document Type: Article

References (70)

1. Walker RI, Willemze R. Neutrophil kinetics and the regulation of granulopoiesis. Rev Infect Dis 1980;2:282-292.
2. Hampton MB, Kettle AJ, Winterbourn CC. Inside the neutrophil phagosome: Oxidants, myeloperoxidase, and bacterial killing. Blood 1998;92:3007-3017.
3. Klebanoff SJ, Coombs RW. Viricidal effect of polymorphonuclear leukocytes on human immunodeficiency virus-1. Role of the myeloperoxidase system. J Clin Invest 1992;89:2014-2017.
4. Segal, AW. How neutrophils kill microbes. Annu Rev Immunol 2005;23:197-223.
5. Babior BM, Lambeth JD, Nauseef W. The neutrophil NADPH oxidase. Arch Biochem Biophys 2002;397:342-344.
6. Sabroe I, Jones EC, Usher LR et al. Toll-like receptor (TLR) 2 and TLR4 in human peripheral blood granulocytes: A critical role for monocytes in leukocyte lipopolysaccharide responses. J Immunol 2002;168:4701-4710.
7. Sabroe I, Prince LR, Jones EC et al. Selective roles for Toll-like receptor (TLR) 2 and TLR4 in the regulation of neutrophil activation and life span. J Immunol 2003;170:5268-5275.
8. Kurt-Jones EA, Mandell L, Whitney C et al. Role of toll-like receptor 2 (TLR2) in neutrophil activation: GM-CSF enhances TLR2 expression and TLR2-mediated interleukin 8 responses in neutrophils. Blood 2002;100:1860-1868.
9. Hayashi F, Means TK, Luster AD. Toll-like receptors stimulate human neutrophil function. Blood 2003;102:2660-2669.
10. Sabroe I, Jones EC, Whyte MK et al. Regulation of human neutrophil chemokine receptor expression and function by activation of Toll-like receptors 2 and 4. Immunology 2005;115:90-98.
11. Atallah E, Schiffer CA. Granulocyte transfusion. Curr Opin Hematol 2006;13:45-49.
12. Bishton M, Chopra R. The role of granulocyte transfusions in neutropenic patients. Br J Haematol 2004;127:501-508.
13. Ofran Y, Avivi I, Oliven A et al. Granulocyte transfusions for neutropenic patients with life-threatening infections: A single centre experience in 47 patients, who received 348 granulocyte transfusions. Vox Sang 2007;93:363-369.
14. Haylock DN, To LB, Dowse TL et al. Ex vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage. Blood 1992;80:1405-1412.
15. Slater NJ, Yamaguchi M, Rothwell DG et al. The human granulocyte/ macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence. Br J Haematol 2003;122:150-158.
16. Hino M, Suzuki K, Yamane T et al. Ex vivo expansion of mature human neutrophils with normal functions from purified peripheral blood CD34+ haematopoietic progenitor cells. Br J Haematol 2000;109:314-321.
17. Evans CA, Pierce A, Winter SA et al. Activation of granulocyte-macrophage colony-stimulating factor and interleukin-3 receptor subunits in a multipotential hematopoietic progenitor cell line leads to differential effects on development. Blood 1999;94:1504-1514.
18. Edvardsson L, Dykes J, Olsson ML et al. Clonogenicity, gene expression and phenotype during neutrophil versus erythroid differentiation of cytokine-stimulated CD34+ human marrow cells in vitro. Br J Haematol 2004;127:451-463.
19. Tura O, Barclay GR, Roddie H et al. Optimal ex vivo expansion of neutrophils from PBSC CD34+ cells by a combination of SCF, Flt3-L and G-CSF and its inhibition by further addition of TPO. J Transl Med 2007;5:53.
20. Paquette RL, Gonzales E, Yoshimura R et al. Ex vivo expansion and differentiation of unselected peripheral blood progenitor cells in serum-free media. J Hematother 1998;7:481-491.
21. Reiffers J, Cailliot C, Dazey B et al. Abrogation of post-myeloablative chemotherapy neutropenia by ex-vivo expanded autologous CD34-positive cells. Lancet 1999;354:1092-1093.
22. Prince HM, Simmons PJ, Whitty G et al. Improved haematopoietic recovery following transplantation with ex vivo-expanded mobilized blood cells. Br J Haematol 2004;126:536-545.
23. Terada Y, Hato F, Sakamoto C et al. Thrombopoietin stimulates ex vivo expansion of mature neutrophils in the early stages of differentiation. Ann Hematol 2003;82:671-676.
24. Huang LT, Paredes CJ, Papoutsakis ET et al. Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytes. Physiol Genomics 2007;31:114-125.
25. Miles A, Misra S. The estimation of the bactericidal power of the blood. J Hyg 1938;38:732-749.
26. Hampton MB, Vissers MC, Winterbourn CC. A single assay for measuring the rates of phagocytosis and bacterial killing by neutrophils. J Leukoc Biol 1994;55:147-152.
27. Jounblat R, Kadioglu A, Iannelli F et al. Binding and agglutination of Streptococcus pneumoniae by human surfactant protein D (SP-D) vary between strains, but SP-D fails to enhance killing by neutrophils. Infect Immun 2004;72:709-716.
28. Chen Y, Hashiguchi N, Yip L et al. Hypertonic saline enhances neutrophil elastase release through activation of P2 and A3 receptors. Am J Physiol Cell Physiol 2006;290:C1051-C1059.
29. Parker LC, Jones EC, Prince LR et al. Endotoxin tolerance induces selective alterations in neutrophil function. J Leukoc Biol 2005;78:1301-1305.
30. Li FQ, Horwitz M. Characterization of mutant neutrophil elastase in severe congenital neutropenia. J Biol Chem 2001;276:14230-14241.
31. Lindmark A, Persson AM, Olsson I. Biosynthesis and processing of cathepsin G and neutrophil elastase in the leukemic myeloid cell line U-937. Blood 1990;76:2374-2380.
32. Garwicz D, Lindmark A, Persson AM et al. On the role of the proform-conformation for processing and intracellular sorting of human cathepsin G. Blood 1998;92:1415-1422.
33. Hasilik A, Pohlmann R, Olsen RL et al. Myeloperoxidase is synthesized as larger phosphorylated precursor. EMBO J 1984;3:2671-2676.
34. Lemansky P, Gerecitano-Schmidek M, Das RC et al. Targeting myeloperoxidase to azurophilic granules in HL-60 cells. J Leukoc Biol 2003;74:542-550.
35. Nauseef WM, Olsson I, Arnljots K. Biosynthesis and processing of myeloperoxidase - A marker for myeloid cell differentiation. Eur J Haematol 1988;40:97-110.
36. Nauseef WM, Root RK, Malech HL. Biochemical and immunologic analysis of hereditary myeloperoxidase deficiency. J Clin Invest 1983;71:1297-1307.
37. Olsen RL, Little C. Studies on the subunits of human myeloperoxidase. Biochem J 1984;222:701-709.
38. Olsson I, Olofsson T, Odeberg H. Myeloperoxidase-mediated iodination in granulocytes. Scand J Haematol 1972;9:483-491.
39. Andersen MR, Atkin CL, Eyre HJ. Intact form of myeloperoxidase from normal human neutrophils. Arch Biochem Biophys 1982;214:273-283.
40. Taylor KL, Pohl J, Kinkade JM Jr. Unique autolytic cleavage of human myeloperoxidase. Implications for the involvement of active site MET409. J Biol Chem 1992;267:25282-25288.
41. Sørensen O, Arnljots K, Cowland JB et al. The human antibacterial cathelicidin, hCAP-18, is synthesized in myelocytes and metamyelocytes and localized to specific granules in neutrophils. Blood 1997;90:2796-2803.
42. Oren Z, Lerman JC, Gudmundsson GH et al. Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: Relevance to the molecular basis for its non-cell-selective activity. Biochem J 1999;341:501-513.
43. Sabroe I, Dower SK, Whyte MK. The role of Toll-like receptors in the regulation of neutrophil migration, activation, and apoptosis. Clin Infect Dis 2005;41(suppl 7):S421-S426.
44. Fan J, Malik AB. Toll-like receptor-4 (TLR4) signaling augments chemokine-induced neutrophil migration by modulating cell surface expression of chemokine receptors. Nat Med 2003;9:315-321.
45. Zarco MA, Ribera JM, Urbano-Ispizua A et al. Phenotypic changes in neutrophil granulocytes of healthy donors after G-CSF administration. Haematologica 1999;84:874-878.
46. O'Mahony DS, Pham U, Iyer R et al. Differential constitutive and cytokine-modulated expression of human Toll-like receptors in primary neutrophils, monocytes, and macrophages. Int J Med Sci 2008;5:1-8.
47. Gunsilius E, Gastl G, Petzer AL. Hematopoietic stem cells. Biomed Pharmacother 2001;55:186-194.
48. Sabroe I, Williams TJ, Hébert CA et al. Chemoattractant cross-desensitization of the human neutrophil IL-8 receptor involves receptor internalization and differential receptor subtype regulation. J Immunol 1997;158:1361-1369.
49. Samanta AK, Oppenheim JJ, Matsushima K. Interleukin 8 (monocyte-derived neutrophil chemotactic factor) dynamically regulates its own receptor expression on human neutrophils. J Biol Chem 1990;265:183-189.
50. Manna SK, Bhattacharya C, Gupta SK et al. Regulation of interleukin-8 receptor expression in human polymorphonuclear neutrophils. Mol Immunol 1995;32:883-893.
51. Shen L, Smith JM, Shen Z et al. Differential regulation of neutrophil chemotaxis to IL-8 and fMLP by GM-CSF: Lack of direct effect of oestradiol. Immunology 2006;117:205-212.
52. Faurschou M, Borregaard N. Neutrophil granules and secretory vesicles in inflammation. Microbes Infect 2003;5:1317-1327.
53. Segal AW, Dorling J, Coade S. Kinetics of fusion of the cytoplasmic granules with phagocytic vacuoles in human polymorphonuclear leukocytes. Biochemical and morphological studies. J Cell Biol 1980;85:42-59.
54. Donini M, Fontana S, Savoldi G et al. G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms. Blood 2007;109:4716-4723.
55. Belaaouaj A, McCarthy R, Baumann M et al. Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis. Nat Med 1998;4:615-618.
56. Belaaouaj A, Kim KS, Shapiro SD. Degradation of outer membrane protein A in Escherichia coli killing by neutrophil elastase. Science 2000;289:1185-1188.
57. Mikami T, Eguchi M, Kurosawa H et al. Ultrastructural and cytochemical characterization of human cord blood cells. Med Electron Microsc 2002;35:96-101.
58. Wagner C, Stegmaier S, Hansch GM. Expression of granzyme B in peripheral blood polymorphonuclear neutrophils (PMN), myeloid cell lines and in PMN derived from haemotopoietic stem cells in vitro. Mol Immunol 2008;45:1761-1766.
59. Scheinecker C, Strobl H, Fritsch G et al. Granulomonocyte-associated lysosomal protein expression during in vitro expansion and differentiation of CD34+ hematopoietic progenitor cells. Blood 1995;86:4115-4123.
60. Garwicz D, Lennartsson A, Jacobsen SE et al. Biosynthetic profiles of neutrophil serine proteases in a human bone marrow-derived cellular myeloid differentiation model. Haematologica 2005;90:38-44.
61. Strobl H, Takimoto M, Majdic O et al. Myeloperoxidase expression in CD34+ normal human hematopoietic cells. Blood 1993;82:2069-2078.
62. Zhang DE, Zhang P, Wang ND et al. Absence of granulocyte colony-stimulating factor signaling and neutrophil development in CCAAT enhancer binding protein alpha-deficient mice. Proc Natl Acad Sci U S A 1997;94:569-574.
63. Yamanaka R, Barlow C, Lekstrom-Himes J et al. Impaired granulopoiesis, myelodysplasia, and early lethality in CCAAT/enhancer binding protein epsilon-deficient mice. Proc Natl Acad Sci U S A 1997;94:13187-13192.
64. Anderson KL, Smith KA, Pio F et al. Neutrophils deficient in PU. 1 do not terminally differentiate or become functionally competent. Blood 1998;92:1576-1585.
65. Dahl R, Walsh JC, Lancki D et al. Regulation of macrophage and neutrophil cell fates by the PU. 1:C/EBPalpha ratio and granulocyte colony-stimulating factor. Nat Immunol 2003;4:1029-1036.
66. Lieschke GJ, Grail D, Hodgson G et al. Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization. Blood 1994;84:1737-1746.
67. Borregaard N, Cowland JB. Granules of the human neutrophilic polymorphonuclear leukocyte. Blood 1997;89:3503-3521.
68. Dinauer MC, Lekstrom-Himes JA, Dale DC. Inherited neutrophil disorders: Molecular basis and new therapies. Hematology Am Soc Hematol Educ Program 2000:303-318.
69. Kyba M, Daley GQ. Hematopoiesis from embryonic stem cells: Lessons from and for ontogeny. Exp Hematol 2003;31:994-1006.
70. Bauer C, Tamm R, Petschow D et al. Oxygen affinity and allosteric effects of embryonic mouse haemolglobins. Nature 1975;257:333-334.