Simple, rapid and sensitive detection of Orientia tsutsugamushi by loop-isothermal DNA amplification

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 102, Issue 12, 2008, Pages 1239-1246

  Paris, Daniel H ^a,b   Blacksell, Stuart D ^a,b   Newton, Paul N ^b,c   Day, Nicholas P J ^a,b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b CHURCHILL HOSPITAL   (United Kingdom)

^c MAHOSOT HOSPITAL   (Laos)

Author keywords

LAMP; Laos; Loop isothermal PCR; Orientia tsutsugamushi; Scrub typhus; Thailand

Indexed keywords

CHAPERONIN; DNA; HEAT SHOCK PROTEIN;

ARTICLE; BACTERIAL COUNT; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC TEST; DILUTION; GENE AMPLIFICATION; GENE TARGETING; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERMETHOD COMPARISON; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MAJOR CLINICAL STUDY; MOLECULAR WEIGHT; NONHUMAN; ORIENTIA TSUTSUGAMUSHI; PLASMID; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCRUB TYPHUS; SENSITIVITY AND SPECIFICITY; VERO CELL;

ANIMALS; BACTERIAL PROTEINS; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; ORIENTIA TSUTSUGAMUSHI; POLYMERASE CHAIN REACTION; SCRUB TYPHUS; SENSITIVITY AND SPECIFICITY; THAILAND;

BACTERIA (MICROORGANISMS); ORIENTIA TSUTSUGAMUSHI;

EID: 54949158969     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.trstmh.2008.04.040     Document Type: Article

References (26)

1. Al-Soud W.A., and Rådström P. Purification and characterization of PCR-inhibitory components in blood cells. J. Clin. Microbiol. 39 (2001) 485-493
2. Blacksell S.D., Bryant N.J., Paris D.H., Doust J.A., Sakoda Y., and Day N.P. Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion. Clin. Infect. Dis. 44 (2007) 391-401
3. Brown G.W., Shirai A., Rogers C., and Groves M.G. Diagnostic criteria for scrub typhus: probability values for immunofluorescent antibody and Proteus OXK agglutinin titers. Am. J. Trop. Med. Hyg. 32 (1983) 1101-1107
4. Choi Y.J., Jang W.J., Ryu J.S., Lee S.H., Park K.H., Paik H.S., Koh Y.S., Choi M.S., and Kim I.S. Spotted fever group and typhus group rickettsioses in humans, South Korea. Emerg. Infect. Dis. 11 (2005) 237-244
5. Fournier P.E., Dumler J.S., Greub G., Zhang J., Wu Y., and Raoult D. Gene sequence-based criteria for identification of new rickettsial isolates and description of Rickettsia heilongjiangensis sp. nov. J. Clin. Microbiol. 41 (2003) 5456-5465
6. Horinouchi H., Murai K., Okayama A., Nagatomo Y., Tachibana N., and Tsubouchi H. Genotypic identification of Rickettsia tsutsugamushi by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 54 (1996) 647-651
7. Jiang J., Chan T.C., Temenak J.J., Dasch G.A., Ching W.M., and Richards A.L. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am. J. Trop. Med. Hyg. 70 (2004) 351-356
8. Kawamura A., and Tanaka H. Rickettsiosis in Japan. Jpn. J. Exp. Med. 58 (1988) 169-184
9. Kim D.M., Yun N.R., Yang T.Y., Lee J.H., Yang J.T., Shim S.K., Choi E.N., Park M.Y., and Lee S.H. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study. Am. J. Trop. Med. Hyg. 75 (2006) 542-545
10. Lee J.H., Park H.S., Jang W.J., Koh S.E., Kim J.M., Shim S.K., Park M.Y., Kim Y.W., Kim B.J., Kook Y.H., Park K.H., and Lee S.H. Differentiation of rickettsiae by groEL gene analysis. J. Clin. Microbiol. 41 (2003) 2952-2960
11. Luksameetanasan R., Blacksell S.D., Kalambaheti T., Wuthiekanun V., Chierakul W., Chueasuwanchai S., Apiwattanaporn A., Stenos J., Graves S., Peacock S.J., and Day N.P. Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi. Southeast Asian J. Trop. Med. Public Health 38 (2007) 91-96
12. Manosroi J., Chutipongvivate S., Auwanit W., and Manosroi A. Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. Southeast Asian J. Trop. Med. Public Health 34 (2003) 831-838
13. Mori Y., Nagamine K., Tomita N., and Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289 (2001) 150-154
14. Nagamine K., Hase T., and Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell Probes 16 (2002) 223-229
15. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., and Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28 (2000) E63
16. Oaks, S.C., Ridgeway, R.L., Shirai, A., Twartz, J.C., 1983. Scrub Typhus Bulletin No. 21 from the Institute for Medical Research Malaysia, United States Army Medical Research Unit.
17. Paris D.H., Blacksell S.D., Stenos J., Graves S.R., Unsworth N.B., Phetsouvanh R., Newton P.N., and Day N.P. Real-time multiplex PCR assay for detection and differentiation of rickettsiae and orientiae. Trans. R. Soc. Trop. Med. Hyg. 102 (2007) 186-193
18. Park H.S., Lee J.H., Jeong E.J., Kim J.E., Hong S.J., Park T.K., Kim T.Y., Jang W.J., Park K.H., Kim B.J., Kook Y.H., and Lee S.H. Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene. Microbiol. Immunol. 49 (2005) 545-549
19. Parola P., Miller R.S., McDaniel P., Telford S.R., Rolain J.M., Wongsrichanalai C., and Raoult D. Emerging rickettsioses of the Thai-Myanmar border. Emerg. Infect. Dis. 9 (2003) 592-595
20. Phongmany S., Rolain J.M., Phetsouvanh R., Blacksell S.D., Soukkhaseum V., Rasachack B., Phiasakha K., Soukkhaseum S., Frichithavong K., Chu V., Keolouangkhot V., Martinez-Aussel B., Chang K., Darasavath C., Rattanavong O., Sisouphone S., Mayxay M., Vidamaly S., Parola P., Thammavong C., Heuangvongsy M., Syhavong B., Raoult D., White N.J., and Newton P.N. Rickettsial infections and fever, Vientiane. Laos. Emerg. Infect. Dis. 12 (2006) 256-262
21. Poon L.L., Wong B.W., Ma E.H., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S., and Peiris J.S. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin. Chem. 52 (2006) 303-306
22. Singhsilarak T., Leowattana W., Looareesuwan S., Wongchotigul V., Jiang J., Richards A.L., and Watt G. Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. Am. J. Trop. Med. Hyg. 72 (2005) 640-641
23. Sonthayanon P., Chierakul W., Wuthiekanun V., Blacksell S.D., Pimda K., Suputtamongkol Y., Pukrittayakamee S., White N.J., Day N.P., and Peacock S.J. Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction. Am. J. Trop. Med. Hyg. 75 (2006) 1099-1102
24. Suttinont C., Losuwanaluk K., Niwatayakul K., Hoontrakul S., Intaranongpai W., Silpasakorn S., Suwancharoen D., Panlar P., Saisongkorh W., Rolain J.M., Raoult D., and Suputtamongkol Y. Causes of acute, undifferentiated, febrile illness in rural Thailand: results of a prospective observational study. Ann. Trop. Med. Parasitol. 100 (2006) 363-370
25. Walsh D.S., Myint K.S., Kantipong P., Jongsakul K., and Watt G. Orientia tsutsugamushi in peripheral white blood cells of patients with acute scrub typhus. Am. J. Trop. Med. Hyg. 65 (2001) 899-901
26. Wilkinson R., Rowland D., and Ching W.M. Development of an improved rapid lateral flow assay for the detection of Orientia tsutsugamushi-specific IgG/IgM antibodies. Ann. N. Y. Acad. Sci. 990 (2003) 386-390