Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study

American Journal of Tropical Medicine and Hygiene

Volumn 75, Issue 3, 2006, Pages 542-545

  Kim, Dong Min ^b   Na, Ra Yun ^b   Tae, Young Yang ^c   Ji, Hyun Lee ^d   Jong, Tae Yang ^e   Shim, Soo Kyoung ^a   Choi, Eun Na ^a   Park, Mi Yeoun ^a   Lee, Seung Hyun ^f  

^a Korea National Institute of Health   (South Korea)

^b Chosun University College of Medicine   (South Korea)

^c Haenam General Hospital   (South Korea)

^d Jangheung General Hospital   (South Korea)

^e Muan General Hospital   (South Korea)

^f Seonam University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M; PRIMER DNA;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; CLINICAL PRACTICE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DISEASE DURATION; FEMALE; HOSPITAL ADMISSION; HUMAN; IMMUNOASSAY; IMMUNOFLUORESCENCE; MAJOR CLINICAL STUDY; MALE; PASSIVE HEMAGGLUTINATION; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDY; SCRUB TYPHUS; SENSITIVITY AND SPECIFICITY; SEROLOGY; ADOLESCENT; FLUORESCENT ANTIBODY TECHNIQUE; METHODOLOGY; MIDDLE AGED; NUCLEOTIDE SEQUENCE;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BASE SEQUENCE; DNA PRIMERS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; SCRUB TYPHUS;

EID: 33750602942     PISSN: 00029637     EISSN: None     Source Type: Journal    
DOI: 10.4269/ajtmh.2006.75.542     Document Type: Article

References (14)

1. Bozeman GW, Elisberg BL, 1963. Serological diagnosis of scrub typhus by indirect immunofluorescent. Proc Soc Exp Biol Med 112: 568-573.
2. Murai K, Tachibana N, Okayama A, Shishime E, Tsuda K, Oshikawa T, 1992. Sensitivity of polymerase chain reaction assay for Rickettsia tsutsugamushi in patients' blood samples. Microbiol Immunol 36: 1145-1153.
3. Suto T, 1980. Rapid serologic diagnosis of tsutsugamushi disease employing the immunoperoxidase reaction with cell cultured rickettsia. Clin Virol 8: 425-429.
4. Furuya Y, Yoshida Y, Katayama T, Yamamoto S, Kawamura A Jr, 1993. Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction. J Clin Microbiol 31: 1637-1640.
5. Manosroi J, Chutipongvivate S, Auwanit W, Manosroi A, 2003. Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. Southeast Asian J Trop Med Public Health 34: 831-838.
6. World Health Organization, 2004. WHO recommended surveillance standards. 2nd ed. Available at http://www.who.int/emcdocuments/surveillance/docs/ whocdscsrisr992.pdf. Accessed 19 June 2004.
7. Kwok S, Higuchi R, 1989. Avoiding false positives with PCR. Nature 339: 237-238.
8. Robinson DM, Brown G, Gan E, Huxsoll DL, 1976. Adaptation of a microimmunofluorescent test to the study of human Rickettsia tsutsugamushi antibody. Am J Trop Med Hyg 25: 900-905.
9. Kim IS, Seong SY, Woo SG, Choi MS, Kang JS, Chang WH, 1993. Rapid diagnosis of scrub typhus by a passive hemagglutination assay using recombinant 56-kilodalton polypeptides. J Clin Microbiol 31: 2057-2060.
10. Silpapojakul K, Chupuppakarn S, Yuthasompob S, Varachit B, Chaipak D, Borkerd T, Silpapojakul K, 1991. Scrub and murine typhus in children with obscure fever in the tropics. Pediatr Infect Dis 10: 200-203.
11. Eiscenstein BI, 1990. The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis. N Engl J Med 323: 178-183.
12. Kelly DJ, Wong PW, Gan E, Lewis GE Jr, 1988. Comparative evalusion of the indirect immunoperoxidase test for the serodiagnosis of rickettsial disease. Am J Trop Med Hyg 38: 400-406.
13. Kawamura A Jr, Tanaka H, Tamura A, 1995. Tsutsugamushi Disease. Tokyo: University of Tokyo Press.
14. Hanson BA, 1983. Effect of immune serum on infectivity of Rickettsia tsutsugamushi. Infect Immun 42: 341-349.