Microbial dynamics in anaerobic enrichment cultures degrading di-n-butyl phthalic acid ester

FEMS Microbiology Ecology

Volumn 66, Issue 2, 2008, Pages 472-483

  Trably, Eric ^a,b   Batstone, Damien J ^a,c   Christensen, Nina ^a   Patureau, Dominique ^a,b   Schmidt, Jens E ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b LABORATOIRE DE BIOTECHNOLOGIE DE L ENVIRONNEMENT   (France)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Anaerobic; Biodegradation; DBP; FISH; Single strand conformation polymorphism; Sludge

Indexed keywords

BENZALDEHYDE; DI BUTYL PHTHALIC ACID ESTER; PHTHALIC ACID; PHTHALIC ACID DIBUTYL ESTER; RNA 16S; UNCLASSIFIED DRUG;

BIODEGRADATION; COMMUNITY DYNAMICS; DOMINANCE; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; MICROSTRUCTURE; PHTHALATE; POLYMORPHISM; RNA; SLUDGE;

ANAEROBIC BACTERIUM; ANAEROBIC REACTOR; ARTICLE; BIOREMEDIATION; CONTROLLED STUDY; ENRICHMENT CULTURE; FLUORESCENCE IN SITU HYBRIDIZATION; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; SOEHNGENIA SACCHAROLYTICA; STEADY STATE;

ANAEROBIOSIS; BACTERIA; BIODEGRADATION, ENVIRONMENTAL; BIOREACTORS; CULTURE MEDIA; DIBUTYL PHTHALATE; DNA, ARCHAEAL; DNA, BACTERIAL; METHANE; METHANOSARCINALES; MOLECULAR SEQUENCE DATA; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

ARCHAEA; BACTERIA (MICROORGANISMS); METHANOSAETA SP.; SOEHNGENIA SACCHAROLYTICA;

EID: 53949116533     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2008.00570.x     Document Type: Article

References (38)

1. Angelidaki I, Mogensen AS Ahring BK (2000) Degradation of organic contaminants found in organic waste. Biodegradation 11 : 377 383.
2. APHA (1995) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington, DC.
3. Benckriser G Ottow JCG (1982) Metabolism of the plasticizer di-n-butylphthalate by Pseudomonas pseudoalcaligenes under anaerobic conditions, with nitrate as the only electron acceptor. Appl Environ Microbiol 44 : 576 578.
4. CEHR, Center for the Evaluation of risks to Human Reproduction (2000) National Toxicology Program - CEHR monograph on the potential human reproductive and developmental effects of di-n-butyl phthalate (DBP). Available at
5. Chang BV, Liao CS Yuan SY (2005) Anaerobic degradation of diethyl phthalate, di-n-butyl phthalate, and di-(2-ethylhexyl) phthalate from river sediment in Taiwan. Chemosphere 58 : 1601 1607.
6. Chauret C, Iniss WE Mayfield CI (1996) Biotransformation at 10 °C of di-n-butyl phthalate in subsurface microcosms. Ground Water 34 : 791 794.
7. Chen CL, Macarie H, Ramirez I, Olmos A, Ong SL, Monroy O Liu WT (2004) Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalate. Microbiology 150 : 3429 3440.
8. Christensen N, Batstone DJ, He Z, Angelidaki I Schmidt JE (2004) Removal of polycyclic aromatic hydrocarbons (PAHs) from sewage sludge by anaerobic degradation. Water Sci Technol 50 : 237 244.
9. Conklin A, Stensel HD Ferguson J (2006) Growth kinetics and competition between Methanosarcina and Methanosaeta in mesophilic anaerobic digestion. Water Environ Res 78 : 486 496.
10. Daims H, Bruhl A, Amann R, Schleifer KH Wagner M (1999) The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22 : 434 444.
11. Delbes C, Moletta R Godon JJ (2000) Monitoring of activity dynamics of an anaerobic digester bacterial community using 16S rRNA polymerase chain reaction-single-strand conformation polymorphism analysis. Environ Microbiol 2 : 506 515.
12. ECPI, European Council for Plasticizers and Intermediates (2004). Available at
13. Edwards EA Grbic-Galic D (1994) Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl Environ Microbiol 60 : 313 322.
14. Ejlertsson J, Meyerson U Svensson BH (1996) Anaerobic degradation of phthalic acid esters during digestion of municipal solid waste under landfilling conditions. Biodegradation 7 : 345 352.
15. Gavala HN, Alatriste-Mondragon F, Iranpour R Ahring BK (2003) Biodegradation of phthalate esters during the mesophilic anaerobic digestion of sludge. Chemosphere 52 : 673 682.
16. Grotenhuis JT, Smit M, Plugge CM, Xu YS, Van Lammeren AA, Stams AJ Zehnder AJ (1991) Bacteriological composition and structure of granular sludge adapted to different substrates. Appl Environ Microbiol 57 : 1942 1949.
17. Hayes LA, Nevin KP Lovley DR (1999) Role of prior exposure on anaerobic degradation of naphthalene and phenanthrene in marine harbor sediments. Org Geochem 30 : 937 945.
18. Hu XY, Wen B Shan XQ (2003) Survey of phthalate pollution in arable soils in China. J Environ Monit 5 : 649 653.
19. Hugenholtz P, Tyson GW Blackall L (2001) Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence in situ hybridization. Methods in Molecular Biology, Vol. 176 (Lieberman BA, ed), pp. 29 41. Humana Press Inc., Totowa, NJ.
20. Jarosova A (2006) Phthalic acid esters (PAEs) in the food chain. Czech J Food Sci 5 : 223 231.
21. Kim SH, Kim SS, Kwon O, Sohn KH, Kwack SJ, Choi YW, Han SY, Lee MK Park KL (2002) Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells. J Toxicol Environ Health 5-6 : 461 472.
22. Kleerebezem R, Hulshoff Pol LW Lettinga G (1999) Anaerobic degradation of phthalate isomers by methanogenic consortia. Appl Environ Microbiol 65 : 1152 1160.
23. Leclerc M, Delgenes JP Godon JJ (2004) Diversity of the archaeal community in 44 anaerobic digesters as determined by Single Strand Conformation Polymorphism analysis and 16S rDNA sequencing. Environ Microbiol 6 : 809 819.
24. Mougin C, Dappozze F, Brault A, Malosse C, Schmidt JE, Amellal-Nassr N Patureau D (2006) Phthalic acid and benzo[a]pyrene in soil-plant-water systems amended with contaminated sewage sludge. Environ Chem Lett 4 : 201 206.
25. O'Connor OA, Rivera MD Young LY (1989) Toxicity and biodegradation of phthalic acid esters under methanogenic conditions. Environ Toxicol Chem 8 : 569 576.
26. Parshina SN, Kleerebezem R, Van Kempen E, Nozhevnikova AN, Lettinga G Stams AJM (2000) Benzaldehyde conversion by two anaerobic bacteria isolated from an upflow anaerobic sludge bed reactor. Proc Biochem 36 : 423 429.
27. Parshina SN, Kleerebezem R, Sanz JL, Lettinga G, Nozhevnikova AN, Kostrikina NA, Lysenko AM Stams AJM (2003) Soehngenia saccharolytica gen. nov., sp. nov. and Clostridium amygdalinum sp. nov., two novel anaerobic, benzaldehyde-converting bacteria. Int J Syst Evol Microbiol 53 : 1791 1799.
28. Patureau D, Laforie M, Lichtfouse E, Caria G, Denaix L Schmidt JE (2007) Fate of organic pollutants after sewage sludge spreading on agricultural soils: a 30-years field-scale recording. Water Practice Technol 2 : 1 9.
29. Qiu YL, Sekiguchi Y, Imachi H, Kamagata Y, Tseng IC, Cheng SS, Ohashi A Harada H (2004) Identification and isolation of anaerobic, syntrophic phthalate isomer-degrading microbes from methanogenic sludges treating wastewater from terephthalate manufacturing. Appl Environ Microbiol 70 : 1617 1626.
30. Schmidt JE Ahring BK (1996) Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors. Biotech Bioeng 49 : 229 246.
31. Sekiguchi Y, Kamagata Y, Nakamura K, Ohashi A Harada H (1999) Fluorescence in situ hybridization using 16S rRNA-targeted oligonucleotides reveals localization of methanogens and selected uncultured bacteria in mesophilic and thermophilic sludge granules. Appl Environ Microbiol 65 : 1280 1288.
32. Sorensen AH, Winther-Nielsen M Ahring BK (1991) Kinetics of lactate, acetate and propionate in unadapted and lactate-adapted thermophilic, anaerobic sewage sludge: the influence of sludge adaptation for start-up of thermophilic UASB-reactors. Appl Microbiol Biotechnol 34 : 823 827.
33. Stahl DA Amann R (1991) Development and application of nucleic acid probes. Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt E Goodfellow M, eds), pp. 205 248. Academic Press, Chichester, UK.
34. Staples CA, Peterson DR, Parkerton TF Adams WJ (1997) The environmental fate of phthalate esters: a literature review. Chemosphere 35 : 667 749.
35. Wang J, Chen L, Shi H Qian Y (2000) Microbial degradation of phthalic acid esters under anaerobic digestion of sludge. Chemosphere 41 : 1245 1248.
36. Wu JH, Liu WT, Tseng IC Cheng SS (2001) Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system. Microbiology 147 : 373 382.
37. Yin R, Lin XG, Wang SG Zhang HY (2003) Effect of DBP/DEHP in vegetable planted soil on the quality of capsicum fruit. Chemosphere 50 : 801 805.
38. Yuan SY, Liu C, Liao CS Chang BV (2002) Occurrence and microbial degradation of phthalate esters in Taiwan river sediments. Chemosphere 49 : 1295 1299.