Influential factor contributing to the isoform-specific inhibition by ATP of human mitochondrial NAD(P)+-dependent malic enzyme: Functional roles of the nucleotide binding site Lys346

FEBS Journal

Volumn 275, Issue 21, 2008, Pages 5383-5392

  Hsieh, Ju Yi ^a   Liu, Guang Yaw ^b   Hung, Hui Chih ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b CHUNG SHAN MEDICAL UNIVERSITY   (Taiwan)

Author keywords

Allosteric regulation; ATP inhibition; Cofactor specificity; Cooperativity; Mutagenesis

Indexed keywords

ADENOSINE TRIPHOSPHATE; ALANINE; ARGININE; FUMARIC ACID; LYSINE; MALATE DEHYDROGENASE (DECARBOXYLATING); MALIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SERINE;

ALLOSTERISM; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CYTOSOL; ELECTRICITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; GENE MUTATION; HUMAN; MITOCHONDRION; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ADENOSINE TRIPHOSPHATE; ALLOSTERIC REGULATION; AMINO ACID SUBSTITUTION; BINDING SITES; HUMANS; KINETICS; LYSINE; MALATE DEHYDROGENASE; MITOCHONDRIAL PROTEINS; PROTEIN ISOFORMS; STATIC ELECTRICITY;

EID: 53849147880     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2008.06668.x     Document Type: Article

References (45)

1. Hsu RY Pry RA (1980) Kinetic studies of the malic enzyme of pigeon liver. 'Half-of-the-sites' behavior of the enzyme tetramer in catalysis and substrate inhibition. Biochemistry 19, 962 968.
2. Sauer LA, Dauchy RT, Nagel WO Morris HP (1980) Mitochondrial malic enzymes. Mitochondrial NAD(P)+-dependent malic enzyme activity and malate-dependent pyruvate formation are progression-linked in Morris hepatomas. J Biol Chem 255, 3844 3848.
3. Hsu RY (1982) Pigeon liver malic enzyme. Mol Cell Biochem 43, 3 26.
4. Skorkowski EF Storey KB (1990) Regulation of coenzyme utilization by mitochondrial NAD(P)-dependent malic enzyme. Int J Biochem 22, 471 475.
5. Mallick S, Harris BG Cook PF (1991) Kinetic mechanism of NAD:malic enzyme from Ascaris suum in the direction of reductive carboxylation. J Biol Chem 266, 2732 2738.
6. Loeber G, Infante AA, Maurer-Fogy I, Krystek E Dworkin MB (1991) Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J Biol Chem 266, 3016 3021.
7. Liu D, Hwang CC Cook PF (2002) Alternative substrates for malic enzyme: oxidative decarboxylation of l-aspartate. Biochemistry 41, 12200 12203.
8. Tipton PA, Quinn TP, Peisach J Cook PF (1996) Role of the divalent metal ion in the NAD:malic enzyme reaction: an ESEEM determination of the ground state conformation of malate in the E:Mn:malate complex. Protein Sci 5, 1648 1654.
9. Hung HC, Chang GG, Yang Z Tong L (2000) Slow binding of metal ions to pigeon liver malic enzyme: a general case. Biochemistry 39, 14095 14102.
10. Chang HC, Chou WY Chang GG (2002) Effect of metal binding on the structural stability of pigeon liver malic enzyme. J Biol Chem 277, 4663 4671.
11. Coleman DE, Rao GS, Goldsmith EJ, Cook PF Harris BG (2002) Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 Å resolution. Biochemistry 41, 6928 6938.
12. Yang Z, Zhang H, Hung HC, Kuo CC, Tsai LC, Yuan HS, Chou WY, Chang GG Tong L (2002) Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme. Protein Sci 11, 332 341.
13. Tao X, Yang Z Tong L (2003) Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism. Structure 11, 1141 1150.
14. Xu Y, Bhargava G, Wu H, Loeber G Tong L (1999) Crystal structure of human mitochondrial NAD(P)+dependent malic enzyme: a new class of oxidative decarboxylases. Structure 7, R877 R889.
15. Yang Z, Floyd DL, Loeber G Tong L (2000) Structure of a closed form of human malic enzyme and implications for catalytic mechanism. Nat Struct Biol 7, 251 257.
16. Yang Z, Lanks CW Tong L (2002) Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate. Structure 10, 951 960.
17. Chang GG Tong L (2003) Structure and function of malic enzymes, a new class of oxidative decarboxylases. Biochemistry 42, 12721 12733.
18. Rao GS, Coleman DE, Karsten WE, Cook PF Harris BG (2003) Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site. J Biol Chem 278, 38051 38058.
19. Chang GG, Wang JK, Huang TM, Lee HJ, Chou WY Meng CL (1991) Purification and characterization of the cytosolic NADP(+)-dependent malic enzyme from human breast cancer cell line. Eur J Biochem 202, 681 688.
20. Chang GG, Huang TM, Wang JK, Lee HJ, Chou WY Meng CL (1992) Kinetic mechanism of the cytosolic malic enzyme from human breast cancer cell line. Arch Biochem Biophys 296, 468 473.
21. Loeber G, Dworkin MB, Infante A Ahorn H (1994) Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett 344, 181 186.
22. Chou WY, Huang SM, Liu YH Chang GG (1994) Cloning and expression of pigeon liver cytosolic NADP(+)-dependent malic enzyme cDNA and some of its abortive mutants. Arch Biochem Biophys 310, 158 166.
23. Bottacchi E Di Donato S (1983) Skeletal muscle NAD+(P) and NADP+-dependent malic enzyme in Friedreich's ataxia. Neurology 33, 712 716.
24. Taroni F, Gellera C Di Donato S (1987) Evidence for two distinct mitochondrial malic enzymes in human skeletal muscle: purification and properties of the NAD(P)+-dependent enzyme. Biochim Biophys Acta 916, 446 454.
25. Biegniewska A Skorkowski EF (1987) Mitochondrial NADP-dependent malic enzyme of cod heart. Rate of forward and reverse reaction. Comp Biochem Physiol B 86, 731 735.
26. Moreadith RW Lehninger AL (1984) The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme. J Biol Chem 259, 6215 6221.
27. Teller JK, Fahien LA Davis JW (1992) Kinetics and regulation of hepatoma mitochondrial NAD(P) malic enzyme. J Biol Chem 267, 10423 10432.
28. Hsieh JY, Liu GY, Chang GG Hung HC (2006) Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362. J Biol Chem 281, 23237 23245.
29. Mariash CN, McSwigan CR, Towle HC, Schwartz HL Oppenheimer JH (1981) Glucose and triiodothyronine both induce malic enzyme in the rat hepatocyte culture: evidence that triiodothyronine multiplies a primary glucose-generated signal. J Clin Invest 68, 1485 1490.
30. Moreadith RW Lehninger AL (1984) Purification, kinetic behavior, and regulation of NAD(P)+ malic enzyme of tumor mitochondria. J Biol Chem 259, 6222 6227.
31. Wasilenko WJ Marchok AC (1985) Malic enzyme and malate dehydrogenase activities in rat tracheal epithelial cells during the progression of neoplasia. Cancer Lett 28, 35 42.
32. Chendrimada TP, Freeman ME Davis AJ (2007) Dietary nitrogen intake regulates hepatic malic enzyme messenger ribonucleic acid expression. Poult Sci 86, 1980 1987.
33. Hung HC, Kuo MW, Chang GG Liu GY (2005) Characterization of the functional role of allosteric site residue Asp102 in the regulatory mechanism of human mitochondrial NAD(P)+-dependent malate dehydrogenase (malic enzyme). Biochem J 392, 39 45.
34. Mandella RD Sauer LA (1975) The mitochondrial malic enzymes. I. Submitochondrial localization and purification and properties of the NAD(P)+-dependent enzyme from adrenal cortex. J Biol Chem 250, 5877 5884.
35. Zolnierowicz S, Swierczynski J Zelewski L (1988) Purification and properties of the NAD(P)-dependent malic enzyme from human placental mitochondria. Biochem Med Metab Biol 39, 208 216.
36. Hung HC, Chien YC, Hsieh JY, Chang GG Liu GY (2005) Functional roles of ATP-binding residues in the catalytic site of human mitochondrial NAD(P)+-dependent malic enzyme. Biochemistry 44, 12737 12745.
37. Sauer LA Dauchy RT (1978) Identification and properties of the nicotinamide adenine dinucleotide (phosphate)+-dependent malic enzyme in mouse ascites tumor mitochondria. Cancer Res 38, 1751 1756.
38. Hsu WC, Hung HC, Tong L Chang GG (2004) Dual functional roles of ATP in the human mitochondrial malic enzyme. Biochemistry 43, 7382 7390.
39. Bhargava G, Mui S, Pav S, Wu H, Loeber G Tong L (1999) Preliminary crystallographic studies of human mitochondrial NAD(P)(+)-dependent malic enzyme. J Struct Biol 127, 72 75.
40. Karsten WE, Pais JE, Rao GS, Harris BG Cook PF (2003) Ascaris suum NAD-malic enzyme is activated by l-malate and fumarate binding to separate allosteric sites. Biochemistry 42, 9712 9721.
41. Kuo CC, Tsai LC, Chin TY, Chang GG Chou WY (2000) Lysine residues 162 and 340 are involved in the catalysis and coenzyme binding of NADP(+)-dependent malic enzyme from pigeon. Biochem Biophys Res Commun 270, 821 825.
42. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248 254.
43. Altschul SF, Boguski MS, Gish W Wootton JC (1994) Issues in searching molecular sequence databases. Nat Genet 6, 119 129.
44. Thompson JD, Higgins DG Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673 4680.
45. Wallace AC, Laskowski RA Thornton JM (1995) LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng 8, 127 134.