Production of monoclonal antibody specific for bottlenose dolphin neutrophils and its application to cell separation

Developmental and Comparative Immunology

Volumn 33, Issue 1, 2009, Pages 14-17

  Kato, Masako ^a   Itou, Takuya ^a   Nagatsuka, Nobuyuki ^b   Sakai, Takeo ^a  

^a NIHON UNIVERSITY   (Japan)

^b Shinagawa Aquarium   (Japan)

Author keywords

Eosinophil; Magnetic activated cell sorting; Monoclonal antibody; Neutrophil; Polymorphonuclear leukocytes; Tursiops truncatus

Indexed keywords

MONOCLONAL ANTIBODY; REAGENT;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; BOTTLENOSE DOLPHIN; CELL FUSION; CELL ISOLATION; CELL SELECTION; CELL SEPARATION; CONTROLLED STUDY; EOSINOPHIL; FEMALE; MALE; MOUSE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RAT;

BOVINAE; RATTUS NORVEGICUS; TURSIOPS; TURSIOPS TRUNCATUS;

ANIMALS; ANTIBODIES, MONOCLONAL; BOTTLE-NOSED DOLPHIN; CELL SEPARATION; CELLS, CULTURED; HYBRIDOMAS; LYMPH NODES; MICE; NEUTROPHILS; RATS; RATS, WISTAR;

EID: 53849122629     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2008.08.003     Document Type: Article

References (28)

1. Smith J.A. Neutrophils, host defense, and inflammation: a double-edged sword. J Leukoc Biol 56 (1994) 672-686
2. Itou T., Sugisawa H., Inoue Y., Jinbo T., and Sakai T. Oxygen radical generation and expression of NADPH oxidase genes in bottlenose dolphin (Tursiops truncatus) neutrophils. Dev Comp Immunol 25 (2001) 47-53
3. Shiraishi R., Itou T., Sugisawa H., Shoji Y., Endo T., and Sakai T. The respiratory burst activity of bottlenose dolphin neutrophils elicited by several stimulants. J Vet Med Sci 64 (2002) 711-714
4. Woodard J.C., Zam S.G., Caldwell D.K., and Caldwell M.C. Some parasitic diseases of dolphins. Pathol Vet 6 (1969) 257-272
5. Blanchard-Channell M., Moore P.F., and Stott J.L. Characterization of monoclonal antibodies specific for equine homologues of CD3 and CD5. Immunology 82 (1994) 548-554
6. De Guise S., Erickson K., Blanchard M., Dimolfetto L., Lepper H., Wang J., Stott J.L., and Ferrick D.A. Characterization of a monoclonal antibody that recognizes a lymphocyte surface antigen for the cetacean homologue to CD45R. Immunology 94 (1998) 207-212
7. De Guise S., Erickson K., Blanchard M., DiMolfetto L., Lepper H.D., Wang J., Stott J.L., and Ferrick D.A. Monoclonal antibodies to lymphocyte surface antigens for cetacean homologues to CD2, CD19 and CD21. Vet Immunol Immunopathol 84 (2002) 209-221
8. Cotter M.J., Norman K.E., Hellewell P.G., and Ridger V.C. A novel method for isolation of neutrophils from murine blood using negative immunomagnetic separation. Am J Pathol 159 (2001) 473-481
9. Jareo P.W., Preheim L.C., Snitily M.U., and Gentry M.J. Use of magnetic cell sorting to isolate blood neutrophils from rats. Lab Anim Sci 47 (1997) 414-418
10. Semple J.W., Allen D., Chang W., Castaldi P., and Freedman J. Rapid separation of CD4+ and CD19+ lymphocyte populations from human peripheral blood by a magnetic activated cell sorter (MACS). Cytometry 14 (1993) 955-960
11. Hansel T.T., De Vries I.J., Iff T., Rihs S., Wandzilak M., Betz S., Blaser K., and Walker C. An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J Immunol Methods 145 (1991) 105-110
12. Stanciu L.A., Shute J., Holgate S.T., and Djukanovic R. Production of IL-8 and IL-4 by positively and negatively selected CD4+ and CD8+ human T cells following a four-step cell separation method including magnetic cell sorting (MACS). J Immunol Methods 189 (1996) 107-115
13. Inoue Y., Itou T., Oike T., and Sakai T. Cloning and sequencing of the bottle-nosed dolphin (Tursiops truncatus) interferon-gamma gene. J Vet Med Sci 61 (1999) 939-942
14. Kishiro Y., Kagawa M., Naito I., and Sado Y. A novel method of preparing rat-monoclonal antibody-producing hybridomas by using rat medial iliac lymph node cells. Cell Struct Funct 20 (1995) 151-156
15. St. Aubin D.J., DeGuise S., Richard P.R., Smith T.G., and Geraci J.R. Hematology and plasma chemistry as indicators of health and ecological status in beluga whales, Delphinapterus leucas. Arctic 54 (2001) 317-331
16. Goldstein J.D., Reese E., Reif J.S., Varela R.A., McCulloch S.D., Defran R.H., Fair P.A., Bossart G.D., and Hansen L. Hematologic, biochemical, and cytologic findings from apparently healthy atlantic bottlenose dolphins (Tursiops truncatus) inhabiting the Indian River Lagoon, Florida, USA. J Wildl Dis 42 (2006) 447-454
17. Jain NC. Schalm's Veterinary Hematology. 4th edition; 1986.
18. Cornell L.H. Hematology and clinical chemistry values in the killer whale, Orcinus orca L. J Wildl Dis 19 (1983) 259-264
19. Shirai K., and Sakai T. Haematological findings in captive dolphins and whales. Aust Vet J 75 (1997) 512-514
20. Sokolova O.V. Peculiarities of phagocytosis in bottlenose dolphin (Tursiops truncatus) during the period of adaptation to the captivity conditions. Dokl Biol Sci 403 (2005) 263-266
21. Shirai K., Sakai T., Fukuda M., and Oike T. A monoclonal antibody against dolphin lymphocytes (6E9) which recognizes bovine MHC class II antigens. J Vet Med Sci 60 (1998) 291-293
22. Taylor B.C., Brotheridge R.M., Jessup D.A., and Stott J.L. Measurement of serum immunoglobulin concentration in killer whales and sea otters by radial immunodiffusion. Vet Immunol Immunopathol 89 (2002) 187-195
23. De Guise S., Erickson K., Blanchard M., DiMolfetto L., Lepper H.D., Stott J.L., and Ferrick D.A. Characterization of F21.A, a monoclonal antibody that recognize a leucocyte surface antigen for killer whale homologue to beta-2 integrin. Vet Immunol Immunopathol 97 (2004) 195-206
24. Hansel T.T., Pound J.D., Pilling D., Kitas G.D., Salmon M., Gentle T.A., Lee S.S., and Thompson R.A. Purification of human blood eosinophils by negative selection using immunomagnetic beads. J Immunol Methods 122 (1989) 97-103
25. Miltenyi S., Muller W., Weichel W., and Radbruch A. High gradient magnetic cell separation with MACS. Cytometry 11 (1990) 231-238
26. Rozell M.D., Erger R.A., and Casale T.B. Isolation technique alters eosinophil migration response to IL-8. J Immunol Methods 197 (1996) 97-107
27. Zahler S., Kowalski C., Brosig A., Kupatt C., Becker B.F., and Gerlach E. The function of neutrophils isolated by a magnetic antibody cell separation technique is not altered in comparison to a density gradient centrifugation method. J Immunol Methods 200 (1997) 173-179
28. Malm-Erjefalt M., Stevens T.R., Persson C.G., and Erjefalt J.S. Discontinuous Percoll gradient centrifugation combined with immunomagnetic separation obviates the need for erythrocyte lysis and yields isolated eosinophils with minimal granule abnormalities. J Immunol Methods 288 (2004) 99-109