The function of neutrophils isolated by a magnetic antibody cell separation technique is not altered in comparison to a density gradient centrifugation method

Journal of Immunological Methods

Volumn 200, Issue 1-2, 1997, Pages 173-179

  Zahler, Stefan ^a   Kowalski, Christian ^a   Brosig, Andreas ^a   Kupatt, Christian ^a   Becker, Bernhard F ^a   Gerlach, Eckehart ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

adhesion; CD11b; flow cytometry; oxidative burst; Percoll; polymorphonuclear neutrophil

Indexed keywords

CD11B ANTIGEN; CD15 ANTIGEN;

ADULT; ARTICLE; CELL SEPARATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; NEUTROPHIL; NORMAL HUMAN; PRIORITY JOURNAL; THROMBOCYTE;

CENTRIFUGATION, DENSITY GRADIENT; HUMANS; IMMUNOMAGNETIC SEPARATION; MACROPHAGE-1 ANTIGEN; NEUTROPHILS;

EID: 0031013210     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00206-2     Document Type: Article

References (15)

1. Bateman, J., Parida, S.K. and Nash, G.B. (1993) Neutrophil integrin assay for clinical studies. Cell. Biochem. Funct. 11, 87-91.
2. Fantone, J.C. and Ward, P.A. (1982) Role of oxygen-derived free radicals and metabolites in leukocyte dependent inflammatory reactions. Am. J. Pathol. 107, 397-418.
3. Fearon, D.T. and Collins, L.A. (1983) Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J. Immunol. 130, 370-375.
4. Forsyth, K.D. and Levinsky, R.J. (1990) Preparative procedures of cooling and re-warming increase leukocyte integrin expression and function on neutrophils. J. Immunol. Methods 128, 159-163.
5. Glasser, L. and Fiederlein, R.L. (1990) The effects of various cell separation procedures on assays of neutrophil function. Am. J. Clin. Pathol. 93, 662-669.
6. Hansel, T., DeVries, I.J., Iff, T., Rihs, S., Wandzilak, M., Betz, S., Blaser, K. and Walker, C. (1991) An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J. Immunol. Methods 145, 105-110.
7. Haslett, C., Guthrie, L.A., Kopaniak, M.M., Johnston, R.B. and Henson, P.M. (1985) Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide. Am. J. Pathol. 119, 101-110.
8. Koegler, G., Capdeville, A.B., Hauch, M., Bruester, H.T., Goebel, U., Wernet, P. and Burdach, S. (1990) High efficiency of a new immunological magnetic cell sorting method for T-cell depletion of human bone marrow. Bone Marrow Transplant. 6, 163-168.
9. Kuijpers, T.W., Tool, A.T., van der Schoot, C.E., Ginsel, L.A., Onderwater, J.J., Roos, D. and Verhoeven, A.J. (1991) Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood 78, 1105-1111.
10. McAfee, J.G., Subramanian, G. and Gagne, G. (1984) Technique of leukocyte harvesting and labelling: problems and perspectives. Sem. Nuclear Med. 14, 83-106.
11. Miltenyi, S., Müller, W., Weichel, W. and Radbruch, A. (1990) High gradient magnetic cell separation with MACS. Cytometry 11, 231-238.
12. Raschke, P. and Becker, B.F. (1995) Adenosine and PAF dependent mechanisms lead to myocardial reperfusion injury by neutrophils after brief ischemia. Cardiovasc. Res. 29, 569-576.
13. Raschke, P., Massoudy, P and Becker, B.F. (1995) Taurine protects the heart from neutrophil-induced reperfusion injury. Free Rad. Biol. Med. 19, 461-471.
14. Venaille, T.J., Misso, N.L., Philips, M.J., Robinson, B.W. and Thompson, P.J. (1994) Effects of different density gradient separation techniques on neutrophil function. Scand. J. Clin. Lab. Invest. 54, 385-391.
15. Weiss, S.J. (1989) Tissue destruction by neutrophils. New Engl. J. Med. 320, 365-376.