Production of IL-8 and IL-4 by positively and negatively selected CD4+ and CD8+ human T cells following a four-step cell separation method including magnetic cell sorting (MACS)

Journal of Immunological Methods

Volumn 189, Issue 1, 1996, Pages 107-115

  Stanciu, Luminita A ^a   Shute, Janis ^a   Holgate, Stephen T ^a   Djukanović, Ratko ^a  

^a SOUTHAMPTON GENERAL HOSPITAL   (United Kingdom)

Author keywords

CD4+ T lymphocyte; CD8+ T lymphocyte; IL 4; IL 8; Magnetic cell separation

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; INTERLEUKIN 2 RECEPTOR; INTERLEUKIN 4; INTERLEUKIN 8;

ADULT; ARTICLE; CELL SEPARATION; HUMAN; HUMAN CELL; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE;

EID: 0030026494     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00240-5     Document Type: Article

References (40)

1. Abts, H., Emmerich, M., Miltenyi, S., Radbruch, A. and Tesch, H. (1989) CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro. J. Immunol. Methods 125, 19-28.
2. Baggiolini, M., Watz, A. and Kunkel, S.L. (1989) Neutrophil-activating peptide-1/interleukin-8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84, 1045-1049.
3. lck) that phosphorylates the CD3 complex. Proc. Natl. Acad. Sci. USA 86, 3277-3281.
4. Bøyum, A., Lovhaug, D., Tresland, L. and Nordlie, E.M. (1991) Separation of leukocytes: improved cell purity by fine adjustments of gradient medium density and osmolality. Scand. J. Immunol. 34, 697-712.
5. + T cell infiltration. J. Immunol. 149, 2954-2960.
6. Davis, L.S., McIlraith, M.J., Paecheo, T., Becker, B., Adix, L.M., Thomas, R., Wacholtz, M.C. and Lipsky, P.E. (1994) Assessment of a positive selection technique using an avidin column to isolated human peripheral blood T cell subsets. J. Immunol. Methods 175, 247-257.
7. Del Prete, G.F., De Carli, M., Mastromauro, C., Biagiotti, R., Macchia, D., Falagiani, P., Ricci, M. and Romagnani, S. (1991) Purified protein derivative of Mycobacterium tuberculosis and excretory-secretory antigen(s) of Toxocara canis expand in vitro human T cells with stable and opposite (type 1 T helper or type 2 T helper) profile of cytokine production. J. Clin. Invest. 88, 346-350.
8. lck activation and TCR desensitization independent of TCR tyrosine phosphorylation. J. Immunol. 153, 2905-2917.
9. Hansel, T.T., De Vries, I.J.M., Iff, T., Rihs, S., Wandzilak, M., Betz, S., Blaser, K. and Walker, C. (1991) An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J. Immunol. Methods 145, 105-110.
10. lck. Proc. Natl. Acad. Sci. USA 88, 1996-2000.
11. + T cells inhibits the binding activity of NF-AT, NF-κB and AP-1, three nuclear factors regulating interleukin-2 gene enhancer activity. Eur. J. Immunol. 24, 2646-2652.
12. + T cell. Immunol. Today 15, 107-110.
13. Koller, C.A., King, G.W., Hurtubise, P.E., Sagone, A.L. and LoBuglio, A.F. (1973) Characterization of glass adherent human mononuclear cells. J. Immunol. 111, 1610-1612.
14. Kurtzhals, J.A.L., Hansen, M.B., Hey, A.S. and Poulsen, L.K. (1992) Measurement of antigen-dependent interleukin-4 production by human peripheral blood mononuclear cells. Introduction of an amplification step using ionomycin and phorbol myristate acetate. J. Immunol. Methods 156, 239-245.
15. Lagoo, A.S., Lagoo-Deenadayalan, S., Lorenz, H.-M., Byrne, J., Barber, W.H. and Hardy, K.J. (1994) IL-2, IL-4, and IFN-γ gene expression versus secretion in superantigen-activated T cells. J. Immunol. 152, 1641-1652.
16. Larsen, C.G., Anderson, A.O., Appella, E., Oppenheim, J.J. and Matsushima, K. (1989) The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243, 1464-1466.
17. Lea, T., Smeland, E., Funderud, S., Vartdal, F., Davies, C., Beiske, K. and Ugelstad, J. (1986) Characterization of human mononuclear cells after positive selection with immunomagnetic particles. Scand. J. Immunol. 23, 509-519.
18. Ledbetter, J.A., June, C.H., Grosmaire, L.S. and Rabinovitch, P.S (1987) Crosslinking of surface antigens causes mobilization of intracellular ionized calcium in T lymphocytes. Proc. Natl. Acad. Sci. USA 84, 1384-1388.
19. Ledbetter, J.A., June, C.H., Rabinovitch, P.S, Grossmann, A., Tsu, T.T. and Imboden, J.B. (1988) Signal transduction through CD4 receptors: stimulatory vs. inhibitory activity is regulated by CD4 proximity to the CD3/T cell receptor. Eur. J. Immunol. 18, 525-532.
20. Luo, K. and Sefton, B.M. (1990) Cross-linking of T-cell surface molecules CD4 and CD8 stimulates phosphorylation of the lck tyrosine protein kinase at the autophosphorylation site. Mol. Cell. Biol. 10, 5305-5313.
21. + cells. J. Immunol. Methods 149, 1-10.
22. Mason, D.W., Penhale, W.J., Sedgwick, J.D. (1987) Preparation of lymphocyte subpopulations. In: G.G.B. Klaus (Ed.), Lymphocytes, A Practical Approach. IRL Press, Oxford, 35-54.
23. Miltenyi, S., Muller, W., Weichel, W. and Radbruch, A. (1990) High gradient magnetic cell separation with MACS. Cytometry 11, 231-238.
24. Morimoto, C., Letvin, N.L., Distaso, J.A., Aldrich, W.R. and Schlossman, S.F. (1985) The isolation and characterization of the human suppressor inducer T cell subset. J. Immunol. 134, 1508-1515.
25. + T lymphocytes by inhibiting interleukin 2 mRNA. Proc. Natl. Acad. Sci. USA 87, 2379-2383.
26. + T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE. J. Exp. Med. 181, 423-428.
27. + helper T cells from atopic subjects. Eur. J. Immunol. 22, 1615-1620.
28. Paul, W.E. (1991) Interleukin-4: a prototypic immunoregulatory lymphokine. Blood 77, 1859-1870.
29. Pflueger, E., Mueller, E.A. and Anderer, F.A. (1990) Preservation of cytotoxic function during multi-cycle immunomagnetic cell separations of human NK cells using a new type of magnetic bead. J. Immunol. Methods 129, 165-173.
30. H2-like bronchoalveolar T-lymphocyte population in atopic asthma. New Engl. J. Med. 326, 298-304.
31. Salgame, P., Abrams, J.S., Clayberger, C., Goldstein, H., Convit, J., Modlin, R.M. and Bloom, B.R. (1991) Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones. Science 25, 279-282.
32. Schweizer, R.C., Welmers, B.A.C., Raaijmakers, J.A.M., Zanen, P., Lammers, J.-W.J. and Koenderman, L. (1994) RANTES-and interleukin-8-induced responses in normal human eosinophils: effects of priming with interleukin-5. Blood 83, 3697-3704.
33. + lymphocyte populations from human peripheral blood by a Magnetic Activated Cell Sorter (MACS). Cytometry 14, 955-960.
34. + type 2 T cell subsets in human leishmaniasis have distinct T cell receptor repertoires. J. Immunol. 151, 7095-7104.
35. lck. Cell 55, 301-308.
36. Veillette, A., Zuniga-Pflucker, J.C, Bolen, J.B. and Kruisbeek, A.M. (1989a) Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways. J. Exp. Med. 170, 1671-1680.
37. lck. Nature 338, 257-259.
38. Vitetta, E.S., Fernandez-Botran, R., Myers, C.D. and Sanders, V.M. (1989) Cellular interactions in the humoral immune response. Adv. Immunol. 45, 1-105.
39. Wierenga, E.A., Snoek, M., Bos, J.D., Jansen, H.M. and Kapsenberg, M.L. (1990) Comparison of diversity and function of house dust mite-specific T lymphocyte clones from atopic and non-atopic donors. Eur. J. Immunol. 20, 1519-1526.
40. Wysocki, L.J. and Sato, V.L. (1978) 'Panning' for lymphocytes: A method for cell selection. Proc. Natl. Acad. Sci. USA 75, 2844-2848.