Discontinuous Percoll gradient centrifugation combined with immunomagnetic separation obviates the need for erythrocyte lysis and yields isolated eosinophils with minimal granule abnormalities

Journal of Immunological Methods

Volumn 288, Issue 1-2, 2004, Pages 99-109

  Malm Erjefält, Monika ^a   Stevens, Tim R ^b   Persson, Carl G A ^a   Erjefält, Jonas S ^b  

^a LUND UNIVERSITY HOSPITAL   (Sweden)

^b LUND UNIVERSITY   (Sweden)

Author keywords

Blood; Cell degranulation; Cell separation; Cytoplasmic granules; DAB; Diaminobenzidine tetrahydrochloride; Electron microscopy; Eosinophil peroxidase; Eosinophils; EPO; FCS; fetal calf serum; MACS; Magnetic cell separation system; PBS; Phosphate buffered saline

Indexed keywords

AMMONIUM CHLORIDE; EOSINOPHIL PEROXIDASE; WATER;

ARTICLE; CELL ISOLATION; CELL STRUCTURE; CENTRIFUGATION; CLINICAL PROTOCOL; CONFIDENCE INTERVAL; CONTROLLED STUDY; DENSITY; EOSINOPHIL; HEMOLYSIS; HISTOCHEMISTRY; HUMAN; HUMAN CELL; IMMUNOLOGICAL PROCEDURES; MAGNETISM; NORMAL HUMAN; PRIORITY JOURNAL; PURIFICATION; SEPARATION TECHNIQUE; STANDARDIZATION; TRANSMISSION ELECTRON MICROSCOPY;

CENTRIFUGATION, DENSITY GRADIENT; CYTOPLASMIC GRANULES; EOSINOPHILS; ERYTHROCYTES; HUMANS; IMMUNOMAGNETIC SEPARATION; LEUKOCYTES; MICROSCOPY, ELECTRON;

EID: 2942620721     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.02.008     Document Type: Article

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