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Volumn 18, Issue 20, 2008, Pages 5668-5671

Corrigendum to "Affinity labeling of the proteasome by a belactosin A derived inhibitor" [Bioorg. Med. Chem. Lett. 18 (2008) 5668] (DOI:10.1016/j.bmcl.2008.08.073);Affinity labeling of the proteasome by a belactosin A derived inhibitor

Author keywords

Affinity label; Anti cancer drug; Protease inhibitor; Proteasome

Indexed keywords

BELACTOSIN A; BELACTOSIN A DERIVED INHIBITOR; DANSYL KF 33955; PROTEASOME; UNCLASSIFIED DRUG;

EID: 53449087088     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2008.08.117     Document Type: Erratum
Times cited : (14)

References (18)
  • 7
    • 53449102811 scopus 로고    scopus 로고
    • note
    • ++H): 806.370 (calcd 806.372).
  • 9
    • 53449093288 scopus 로고    scopus 로고
    • note
    • 50 values.
  • 10
    • 53449096789 scopus 로고    scopus 로고
    • note
    • 2. The viability of HeLa cells was tested using an MTT assay after incubation with inhibitors added at various concentrations for 48 h. Treatment with (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl) tetrazolium bromide (MTT) was performed according to the manufacturer's protocol. Absorbance at 570 nm was measured using a plate reader.
  • 12
    • 53449084383 scopus 로고    scopus 로고
    • note
    • HeLa cells were mixed with various concentrations of clasto-lactacystin β-lactone and MG132 and incubated for 1 h at 37 °C in serum-free medium. Next, they were mixed with 1.0 μM dansyl-KF33955 and incubated for another hour. To stop the assay, the cells were collected by centrifugation (15,000g, 5 min), treated with denaturing buffer (60 mM Tris-HCl pH 8.8, 5 M urea, 1 M thiourea, 5 mM EDTA, 1% CHAPS, 1% Triton X-100) at 4 °C, and lysed by sonication. The samples were separated by SDS-PAGE and protein bands on the gel were visualized by SYPRO-Red staining (Invitrogen Co.). Images were acquired using a FLA3000 laser scanner (Fuji Photo Film Co., Ltd., Tokyo, Japan). Following transfer onto a PVDF membrane, immunodetection using an anti-dansyl monoclonal antibody (Santa Cruz Biotechnology) was performed. HRP-conjugated anti-rabbit IgG (MBL) was used as the secondary antibody. Signals were visualized using an ECL Plus detection kit (Amersham Biosciences) and an LAS-1000 Plus image analyzer (Fuji Photo Film Co., Ltd.).
  • 13
    • 33646841837 scopus 로고    scopus 로고
    • note
    • 2, 0.5 mM EDTA, 1 mM dithiothreitol, and 1 mM ATP). The cells were permeabilized by the addition of 0.025% digitonin and incubation on ice for 5 min. The cytosol was "squeeze out" by centrifugation for 15 min at 15,000g. The supernatants were used for the next experiments.
  • 14
    • 53449096492 scopus 로고    scopus 로고
    • note
    • 10


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.