The axial channel of the proteasome core particle is gated by the Rpt2 ATPase and controls both substrate entry and product release

Molecular Cell

Volumn 7, Issue 6, 2001, Pages 1143-1152

  Köhler, Alwin ^a   Cascio, Paolo ^a   Leggett, David S ^a   Woo, Kee Min ^a   Goldberg, Alfred L ^a   Finley, Daniel ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; PROTEASOME; PROTEIN RPT2; UNCLASSIFIED DRUG;

ANTIGEN PRESENTATION; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; ENZYME REGULATION; ENZYME SPECIFICITY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN SECRETION; PROTEIN TRANSPORT; REGULATORY MECHANISM; STRAIN DIFFERENCE; YEAST;

EID: 0034964524     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00274-X     Document Type: Article

References (42)

1. Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum
2. The proteasome: Paradigm of a self-compartmentalizing protease
3. PAN, the proteasome-activating nucleotidase from archaebacteria, is a protein-unfolding molecular chaperone
4. The proteasome
5. The base of the proteasome regulatory complex exhibits chaperone-like activity
6. 26S proteasomes and immunoproteasomes produce mainly N-extended versions of an antigenic peptide
7. Identification, purification, and characterization of a high molecular weight, ATP-dependent activator (PA700) of the 20S proteasome
8. The proteasome, a novel protease regulated by multiple mechanisms
9. Coordinated dual cleavages induced by the proteasome regulator PA28 lead to dominant MHC ligands
10. Decelerated degradation of short peptides by the 20S proteasome
11. The human 26S and 20S proteasomes generate overlapping but different sets of peptide fragments from a model protein substrate
12. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses
13. A subcomplex of the proteasome regulatory particle required for ubiquitin conjugate degradation and related to the COP9-signalosome and eIF3
14. The regulatory particle of the Saccharomyces cerevisiae proteasome
15. A role for the proteasome regulator PA28α in antigen presentation
16. Structure of the 20S proteasome from yeast at 2.4 Å resolution
17. A gated channel into the proteasome core particle
18. The ubiquitin system
19. Activation of the multicatalytic protease
20. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP
21. Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes
22. The different proteolytic sites in mammalian proteasomes allosterically regulate each other's activities, suggesting an ordered ("bite-chew") mechanism for protein degradation
23. The sizes of peptides generated from protein by mammalian 26 and 20S proteasomes
24. Crystal structure of the 20S proteasome from the archeon T. acidophilum at 3.4 Å resolution
25. Distinct proteolytic processes generate the C and N termini of MHC class I-binding peptides
26. Cleavage motifs of the yeast 20S proteasome β subunits deduced from digests of enolase 1
27. Structural features of the 26S proteasome complex
28. Opening doors into the proteasome
29. The proteasome activator 11S REG (PA28) and class I antigen presentation
30. Degradation of cell proteins and the generation of MHC class I-presented peptides
31. Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome
32. Structure and mechanism of ATP-dependent proteases
33. Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20S proteasome
34. Unfolding and internalization of proteins by the ATP-dependent proteases ClpXP and ClpAP
35. Two new proteases in the MHC class I processing pathway
36. Hybrid proteasomes: Induction by interferon-γ and contribution to ATP-dependent proteolysis
37. Recognition of the polyubiquitin signal
38. Global unfolding of a substrate by the Hsp100 chaperone ClpA
39. Existence of a molecular ruler in proteasomes suggested by analysis of degradation products
40. Structural basis for the activation of 20S proteasomes by 11S regulators
41. Structural and functional characterization of the proteasome-activating protein PA26 from Trypanosome brucei
42. An archaebacterial ATPase, homologous to ATPases in the eukaryotic 26S proteasome, activates protein breakdown by 20S proteasomes