Proteolytic processing of oligopeptides containing the target sequences by the recombinant tobacco vein mottling virus Nla proteinase

Molecules and Cells

Volumn 10, Issue 2, 2000, Pages 213-219

  Yoon, Hyun Yee ^a   Hwang, Duk Chul ^b   Choi, Kwan Yong ^b   Song, Byeong Doo ^a  

^a Handong University   (South Korea)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

Substrate Specificity; TVMV NIa Proteinase

Indexed keywords

NIA; NICOTIANA TABACUM; POTYVIRIDAE; TOBACCO VEIN MOTTLING VIRUS;

HYBRID PROTEIN; NI(A) LIKE PROTEIN, VIRUS; NI(A)-LIKE PROTEIN, VIRUS; OLIGOPEPTIDE; PROTEINASE; VIRUS PROTEIN;

ARTICLE; BINDING SITE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PLANT; POTYVIRUS; PROTEIN MOTIF; PROTEIN PROCESSING; TOBACCO; VIROLOGY;

AMINO ACID MOTIFS; BINDING SITES; ENDOPEPTIDASES; KINETICS; OLIGOPEPTIDES; PLANTS, TOXIC; POTYVIRIDAE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TOBACCO; VIRAL PROTEINS;

EID: 0034732435     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10059-000-0213-3     Document Type: Article

References (52)

1. Allison, R. F., Johnston, R. E., and Dougherty, W. G. (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154, 9-20.
2. Anandalakshmi, R., Pruss, G. J., Ge, X., Marathe, R., Mallory, A. C., Smith, T. H., and Vance, V. B. (1998) A viral suppresser of gene silencing in plants. Proc. Natl. Acad. Sci. USA 95, 13079-13084.
3. Atreya, C. D. and Pirone, T. P. (1993) Mutational analysis of the helper component proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc. Natl. Acad. Sci. USA 90, 11919-11923.
4. Atreya, C. D., Atreya, P. L., Thornbury, D. W., and Pirone, T. P. (1992) Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expressed in infected tobacco plants. Virology 191, 106-111.
5. Bazan, J. F. and Fletterick, R. J. (1988) Viral cysteine proteases are homologous to the trypsin-like family of serine proteases; structural and functional implications. Proc. Natl. Acad. Sci. USA 85, 7872-7876.
6. Brigneti, G., Voinnet, O., Li, W.-X., Ji, L.-H., Ding, S.-W., and Baulcornbe, D. C. (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J. 17, 6739-6746.
7. Carrington, J. C., Cary, S. M., and Dougherty, W. G. (1988) Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase. J. Virol. 62, 2313-2320.
8. Carrington, J. C., Cary, S. M., Parks, T. D., and Dougherty, W. G. (1989) A second proteinase encoded by a plant potyvirus genome. EMBO J. 8, 365-370.
9. Carrington, J. C. and Dougherty, W. G. (1987a) Processing of the tobacco etch virus 49K protease requires autoproteolysis. Virology 160, 355-360.
10. Carrington, J. C. and Dougherty, W. G. (1987b) Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J. Virol. 61, 2540-2548.
11. Carrington, J. C. and Dougherty, W. G. (1988) A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc. Natl. Acad. Sci. USA 85, 3391-3395.
12. Carrington, J. C., Haldeman, R., Dolia, V. V., and Restrepo-Hartwig, M. A. (1993) Internal cleavage and trans-proteolytic activities of the VPg-proteinase (NIa) of tobacco etch potyvirus in vivo. J. Virol. 67, 6995-7000.
13. Cronin, S., Verchot, J., Haldeman-Cahill, R., Schaad, M. C., and Carrington, J. C. (1995) Long-distance movement factor: a transport function of the potyvirus helper component-proteinase. Plant Cell 7, 549-559.
14. Domier, L. L., Franklin, K. M., Shahabuddin, M., Hellmann, G. M., Overmyer, J. H., Jiremath, S. T., Siaw, M. F. E., Lomonossoff, G. P., Shaw, J. G., and Rhoads, R. E. (1986) The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Res. 14, 5417-5430.
15. Dougherty, W. G. and Carrington, J. C. (1988) Expression and function of potyviral gene products. Annu. Rev. Phytopathol. 26, 123-143.
16. Dougherty, W. G. and Parks, T. D. (1989) Molecular genetic and biochemical evidence for the involvement of the hepatapeptide cleavage sequence in determining the reaction profile at two tobacco etch virus cleavage sites in cell-free assays. Virology 172, 145-155.
17. Dougherty, W. G. and Parks, T. D. (1991) Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: identification of an internal cleavage site and delimitation of VPg and proteinase. Virology 183, 449-456.
18. Dougherty, W. G. and Semler, B. L. (1993) Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes. Microbiol. Rev. 57, 781-822.
19. Dougherty, W. G., Cary, S. M., and Parks, T. D. (1989) Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171, 356-364.
20. Garcia, J. A., Lain, S., Cervera, M. T., Riechmann, J. L., and Martin, M. T. (1990) Mutational analysis of plum pox potyvirus polyprotein processing by the NIa protease in Escherichia coli. J. Gen. Virol. 71, 2773-2779.
21. Garcia, J. A., Martin, M. T., Cervera, M. T., and Riechmann, J. L. (1992) Proteolytic processing of the plum pox potyvirus polyprotein by the NIa protease at a novel cleavage site Virology 188, 697-703.
22. Garcia, J. A., Riechmann, J. L., and Lain, S. (1989a) Artificial cleavage site recognized by plum pox potyvirus protease in Escherichia coli. J. Virol. 63, 2457-2460.
23. Garcia, J. A., Riechmann, J. L., and Lain, S. (1989b) Proteolytic activity of the plum pox potyvirus NIa-like protein in Escherichia coli. Virology 170, 362-369.
24. Gill, S. C. and von Hippel, P. H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-325.
25. Gorbalenya, A., Donchenko, A. P., Blinov, V. M., and Koonin, E. V. (1989) Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases: a distinct protein superfamily with a common structural fold. FEBS Lett. 243, 103-114.
26. Gough, K. H. and Shukla, D. D. (1993) The nucleotide sequence of Johnsongrass mosaic virus genomic RNA. Intervirology 136, 181-192.
27. Hellmann, G. M., Shaw, J. G., and Rhoads, R. E. (1988) In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease. Virology 163, 554-562.
28. Hwang, D. C. (1997) Cloning, expression, purification and characterization of the nuclear inclusion a (NIa) protease from tobacco vein mottling virus. M. S. Thesis, Pohang University of Science and Technology.
29. Jarayam, Ch., Hill, J. H., and Miller, W. A. (1992) Complete nucleotide sequence of two soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. J. Gen. Virol. 73, 2067-2077.
30. Johansen, E., Rasmussen, O. F., Heide, M., and Borkhardt, B. (1991) The complete nucleotide sequence of pea seed-borne mosaic virus RNA. J. Gen. Virol. 72, 2625-2632.
31. Kasschau, K. D. and Carrington, J. C. (1998) A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell 95, 461-470.
32. Kim, D.-H., Park, Y. S., Kim, S. S., Lew, J., Nam, H. G., and Choi, K. Y. (1995) Expression, purification, and identification of a novel self-cleavage site of the NIa C-term nal 27-kDa protease of turnip mosaic potyvirus C5. Virology 213, 517-525.
33. Kim, D.-H., Hwang, D. C., Kang, B. H., Lew, J., Han, J., Song, B. D., and Choi, K. Y. (1996a) Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity. Virology 226, 183-190.
34. Kim, D.-H., Kang, B. H., Hwang, D. C., Kim, S. S., Kwon, T.-I., and Choi, K. Y. (1996b) Inhibition studies on the nuclear inclusion protein A protease of turnip mosaic potyvirus C5. Mol. Cells 6, 653-658.
35. Kim, D.-H., Han, J., Lew, J., Kim, S. S., Kang, B. H. Hwang, D. C. Jang, D. S., Kim, W., Song, B. D., and Choi, K. Y. (1998) Effects of mutations in the C-terminal region of NIa protease on cis-cleavage between NIa and NIb. Virology 241, 94-100.
36. Klein, P. G., klein, R. R., Rodriguez-Cerezo, E., Hunt, A. G., and Shaw, J. G. (1994) Mutational analysis of the tobacco vein mottling virus genome. Virology 204, 759-769.
37. Lain, S., Riechmann, J. I., and Garcia, J. A. (1989) The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13, 157-172.
38. Matthews, D. A., Smith, W. W., Ferre, R. A., Condon, B., Budahazi, G., Sisson, W., Villafranca, J. E., Janson, C. A., McElroy, H. E., Gribskov, C. L., and Worland, S. (1994) Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA binding site, and means for cleaving precursor polyprotein. Cell 77, 761-771.
39. Murphy, J. F., Rhoads, R. E., Hunt, A. G., and Shaw, J. G. (1990) The VPg of tobacco etch virus RNA is the 49 kDa proteinase or the N-terminal 24 kDa part of the proteinase. Virology 178, 285-288.
40. Nicolas, O. and Laliberte, J.-F. (1992) The complete nucleotide sequence of turnip mosaic potyvirus RNA. J. Gen. Virol. 73, 2785-2793.
41. Parks, T. D., Howard, E. D., Wolpert, T. J., Arp, D. J., and Dougherty, W. G. (1995) Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analysis of the full length and a naturally occurring truncated proteinase form. Virology 210, 194-201.
42. Riechmann, J. L., Lain, S., and Gracia, J. A. (1992) Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73, 1-16.
43. Robaglia, G., Durand-Tardif, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S., and Casse-Delbart, F (1989) Nucleotide sequence of potato virus Y (N strain) genomic RNA J. Gen. Virol. 70, 935-947.
44. Schaad, M. C., Haldeman-Cahill, R., Cronin, S., and Carrington, J. C. (1996) Analysis of the VPg-Proteinase (NIa) encoded by tobacco etch potyvirus: effects of mutations on subcellular transport, proteolytic processing and genome amplification. J. Virol. 70, 7039-7048.
45. Shukla, D. D., Ward, C. W., and Brunt, A. A. (1994) The Potyviridae, CAB International, Wallingford.
46. Thornbury, D. W., Hellmann, G. M., Rhoads, R. E., and Pirone, T. P. (1985) Purification and characterization of potyvirus helper component. Virology 144, 260-267.
47. Udenfriend, S., Stein, S., Böhlen, P., Dariman, W., Leimgruber, W., and Weigele, M. (1972) Fluorescamine: a reagent for assay of umino acids, peptides, proteins, and primary amines in the picomole range. Science 178, 871-872.
48. Vance, V. B., Moore, D., Turpen, T. H., Bracker, A., and Hollowell, V. G. (1992) The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191, 19-30.
49. Verchot, J. and Carrington, J. C. (1995) Evidence that the potyvirus P1 protein functions as an accessory factor for genome amplification. J. Virol. 69, 3668-3674.
50. Verchot, J. E., Koonin, E. V., and Carrington, J. C. (1991) The 35-kDa protein from the N-terminus of a potyviral protein functions as a third virus-encoded proteinase. Virology 185, 527-535.
51. Yeh, S. D., Jan, F.-J., Chian, C.-H., Doong, T.-J., Chen, M.-C., Chung, P.-H., and Bau, H.-J. (1992) Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA. J. Gen. Virol. 73, 2531-2541.
52. Yoon, H. Y., Choi, K. Y., and Song, B. D. (2000) Fluorometric assay of turnip mosaic virus NIa protease. Anal. Biochem. 277, 228-231.