In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites

Journal of Experimental and Clinical Cancer Research

Volumn 27, Issue 1, 2008, Pages

  Sun, Lina ^a,b   Zhang, Ge ^a,c   Li, Zongfang ^a   Song, Tusheng ^a   Huang, Chen ^a   Si, Lusheng ^a  

^a XI'AN JIAOTONG UNIVERSITY   (China)

^b CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^c SHANXI MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ATM PROTEIN; CHECKPOINT KINASE 2; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN E6; PROTEIN P53; SERINE; E6 PROTEIN, HUMAN PAPILLOMAVIRUS TYPE 8; ONCOPROTEIN; PROTEIN SERINE THREONINE KINASE; UNCLASSIFIED DRUG;

ARTICLE; CELL NUCLEUS; CELL STRAIN MCF 7; DNA DAMAGE; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 18; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; VIRUS INFECTION; VIRUS REPLICATION; FEMALE; GENETICS; METABOLISM; PHOSPHORYLATION; TUMOR CELL LINE;

CELL LINE, TUMOR; CELL NUCLEUS; FEMALE; GREEN FLUORESCENT PROTEINS; HUMANS; ONCOGENE PROTEINS, VIRAL; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 52949119973     PISSN: None     EISSN: 17569966     Source Type: Journal    
DOI: 10.1186/1756-9966-27-35     Document Type: Article

References (35)

1. Papillomaviruses in human cancer. H Zur Hausen, Cancer 1987 59 1692 1696 10.1002/1097-0142(19870515)59:10<1692::AID-CNCR2820591003>3.0.CO;2-F 3030526
2. HPV DNA frequency and subset analysis in human breast cancer patients' normal and tumoral tissue samples. M Gumus PF Yumuk T Salepci M Aliustaoglu F Dane M Ekenel G Basaran H Kaya N Barisik NS Turhal, J Exp Clin Cancer Res 2006 25 515 521 17310842
3. Cellular steady-state levels of "high risk" but not "low risk" human papillomavirus (HPV) E6 proteins are increased by inhibition of proteasome-dependent degradation independent of their p53- and E6AP-binding capabilities. E Kehmeier H Rühl B Voland MC Stöppler, Virology 2002 299 72 87 10.1006/viro.2002.1502 12167343
4. The human papillomavirus E6 protein and its contribution to malignant progression. F Mantovani L Banks, Oncogene 2001 20 7874 7887 10.1038/sj.onc.1204869 11753670
5. Human papillomavirus infection and p53 nuclear overexpression in anal canal carcinoma. M Indinnimeo C Cicchini A Stazi E Giarnieri D French MR Limiti C Ghini A Vecchione, J Exp Clin Cancer Res 1999 18 47 52 10374676
6. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. M Scheffner BA Werness JM Huibregtse, Cell 1990 63 1129 1136 10.1016/0092-8674(90)90409-8 2175676
7. HPV 16-E6-mediated degradation of intrinsic p53 is compensated by upregulation of p53 gene expression in normal cervical keratinocytes. Y Kawamata A Mitsuhashi Y Unno S Kado Y Shino K Uesugi O Eguchi J Ishii K Seki S Sekiya H Shirasawa, Int J Oncol 2002 21 561 567 12168100
8. Functional p53 protein in human papillomavirus-positive cancer cells. K Butz L Shahabeddin C Geisen D Spitkovsky A Ullmann F Hoppe-Seyler, Oncogene 1995 10 927 936 7898934
9. Expression of p53 protein related to the presence of human papillomavirus infection in precancer lesions of the larynx. P Fouret D Dabit M Sibony D Alili F Commo JL Saint-Guily P Callard, Am J Pathol 1995 146 599 604 7887442
10. Stability of p53 protein in rat cells transformed by various viral transforming genes. H Inoue G Kondoh TM Sudiro A Hakura, Virology 1992 187 343 347 10.1016/0042-6822(92)90325-J 1736539
11. Schedule-dependent cytotoxicity of 5-fluorouracil and irinotecan in p53 mutant human colon cancer. K Tanaka Y Inoue J Hiro S Yoshiyama Y Toiyama T Eguchi C Miki M Kusunoki, J Exp Clin Cancer Res 2007 26 241 251 17725105
12. Differential induction of transcriptionally active p53 following UV or ionizing radiation: defects in chromosome instability syndromes? X Lu DP Lane, Cell 1993 75 765 778 10.1016/0092-8674(93)90496-D 8242748
13. Genotoxic stress-induced expression of p53 and apoptosis in leukemic clam hemocytes with cytoplasmically sequestered p53. S Böttger E Jerszyk B Low C Walker, Cancer Res 2008 68 777 782 10.1158/0008-5472.CAN-06-0968 18245478
14. The complexity of p53 stabilization and activation. MF Lavin N Gueven, Cell Death Differ 2006 13 941 950 10.1038/sj.cdd.4401925 16601750
15. Inhibition of the ATM/p53 signal transduction pathway by Kaposi's sarcoma-associated herpesvirus interferon regulatory factor 1. YC Shin H Nakamura X Liang P Feng H Chang TF Kowalik JU Jung, J Virol 2006 80 2257 2266 16474133 10.1128/JVI.80.5.2257-2266.2006
16. Modulation of p53 cellular function and cell death by African swine fever virus. AG Granja ML Nogal C Hurtado J Salas ML Salas AL Carrascosa Y Revilla, J Virol 2004 78 7165 7174 15194793 10.1128/JVI.78.13.7165-7174.2004
17. Latent membrane protein 1 of Epstein-Barr virus regulates p53 phosphorylation through MAP kinases. L Li L Guo Y Tao S Zhou Z Wang W Luo D Hu Z Li L Xiao M Tang W Yi SW Tsao Y Cao, Cancer Lett 2007 255 219 231 10.1016/j.canlet.2007.04.014 17582679
18. DNA damage-induced activation of ATM and ATM-dependent signaling pathways. EU Kurz SP Lees-Miller, DNA Repair 2004 3 889 900 10.1016/j.dnarep. 2004.03.029 15279774
19. Enhanced phosphorylation of p53 by ATM in response to DNA damage. S Banin L Moyal S Shieh Y Taya CW Anderson L Chessa NI Smorodinsky C Prives Y Reiss Y Shiloh Y Ziv, Science 1998 281 1674 1677 10.1126/science.281.5383.1674 9733514
20. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. S Matsuoka G Rotman A Ogawa Y Shiloh K Tamai SJ Elledge, Proc Natl Acad Sci USA 2000 97 10389 10394 10973490 10.1073/pnas.190030497
21. High-Risk human papillomavirus E6 protein has two distinct binding sites within p53, of which only one determines degradation. X Li P Coffino, J Virol 1996 70 4509 4516 8676476
22. Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics. P Giannakakou M Nakano KC Nicolaou, Proc Natl Acad Sci USA 2002 99 10855 10860 12145320 10.1073/pnas.132275599
23. Proteomic identification of p53-dependent protein phosphorylation. R Rahman-Roblick U Hellman S Becker, Oncogene 2008 27 4854 4859 10.1038/onc.2008.124 18438429
24. Participation of p53 protein in the cellular response to DNA damage. MB Kastan O Onyekwere D Sidransky B Vogelstein RW Craig, Cancer Res 1991 51 6304 6311 1933891
25. Human papillomavirus 16 E6 expression disrupts the p53-mediated cellular response to DNA damage. TD Kessis RJ Slebos WG Nelson MB Kastan BS Plunkett SM Han AT Lorincz L Hedrick KR Cho, Proc Natl Acad Sci USA 1993 90 3988 3992 8387205 10.1073/pnas.90.9.3988
26. A serine 37 mutation associated with two missense mutations at highly conserved regions of p53 affect pro-apoptotic genes expression in a T-lymphoblastoid drug resistant cell line. C Cinti PP Claudio AD Luca M Cuccurese CM Howard M D'Esposito MG Paggi DL Sala L Azzoni TD Halazonetis A Giordano NM Maraldi, Oncogene 2000 19 5098 5105 10.1038/sj.onc.1203848 11042698
27. Cisplatinum and taxol induce different patterns of p53 phosphorylation. G Damia L Filiberti F Vikhanskaya L Carrassa Y Taya M D'incalci M Broggini, Neoplasia 2001 3 10 16 11326311 10.1038/sj.neo.7900122
28. ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis. N Pabla S Huang QS Mi R Daniel Z Dong, J Biol Chem 2008 283 6572 6583 10.1074/jbc.M707568200 18162465
29. Novel phosphorylation sites of human tumour suppressor protein p53 at Ser20 and Thr18 that disrupt the binding of mdm2 (mouse double minute 2) protein are modified in human cancers. AL Craig L Burch B Vojtesek J Mikutowska A Thompson TR Hupp, Biochem J 1999 342 133 141 10432310 10.1042/0264-6021:3420133
30. Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. S Saito H Yamaguchi Y Higashimoto C Chao Y Xu AJ Fornace Jr E Appella CW Anderson, J Biol Chem 2003 278 37536 37544 10.1074/jbc.M305135200 12860987
31. Protein kinase CK2 is involved in G2 arrest and apoptosis following spindle damage in epithelial cells. M Sayed S Pelech C Wong A Marotta B Salh, Oncogene 2001 20 6994 7005 10.1038/sj.onc.1204894 11704824
32. Differential posttranslational modification of the tumour suppressor proteins Rb and p53 modulate the rates of radiation-induced apoptosis in vivo. M Wallace PJ Coates EG Wright KL Ball, Oncogene 2001 20 3597 3608 10.1038/sj.onc.1204496 11439323
33. Mutation of phosphoserine 389 affects p53 function in vivo. M Hao AM Lowy M Kapoor A Deffie G Liu G Lozano, J Biol Chem 1996 271 29380 29385 10.1074/jbc.271.46.29380 8910602
34. Association of human papillomavirus types 16 and 18 E6 proteins with p53. BA Werness AJ Levine PM Howley, Science 1990 248 76 79 10.1126/science.2157286 2157286
35. The papillomavirus life cycle. J Doorbar, J Clin Virol 2005 32 S7 15 10.1016/j.jcv.2004.12.006 15753007