T-DNA insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus Laccaria bicolor

Microbial Biotechnology

Volumn 1, Issue 3, 2008, Pages 258-269

  Kemppainen, Minna ^a,b   Duplessis, Sebastien ^c   Martin, Francis ^c   Pardo, Alejandro G ^a,b  

^a UNIVERSIDAD NACIONAL DE QUILMES   (Argentina)

^b Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

^c UNIVERSITÉ DE LORRAINE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM MEDIATED GENE TRANSFER; ARTICLE; BACTERIAL STRAIN; BASIDIOMYCETES; DNA INTEGRATION; ECOSYSTEM; ECTOMYCORRHIZA; FOREST; FUNGAL STRAIN; GENE EXPRESSION; GENE INSERTION; GENE SEQUENCE; GENE TRANSFER; GENETIC TRANSCRIPTION; GENOMICS; LACCARIA; LACCARIA BICOLOR; MUTAGENESIS; NONHUMAN; PLASMID; RHIZOBIUM; SYMBIOSIS;

BASE SEQUENCE; DNA, BACTERIAL; GENE TRANSFER TECHNIQUES; GENOME, FUNGAL; LACCARIA; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MYCORRHIZAE; PLASMIDS; RHIZOBIUM;

AGROBACTERIUM; ESCHERICHIA COLI; FUNGI; LACCARIA; LACCARIA BICOLOR;

EID: 52649108786     PISSN: 17517907     EISSN: 17517915     Source Type: Journal    
DOI: 10.1111/j.1751-7915.2008.00029.x     Document Type: Article

References (43)

1. Alonso, J.M., Stepanova, A.N., Leisse, T.J., Kim, C.J., Chen, H., Shinn, P., et al. (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657.
2. Blaise, F., Remy, E., Meyer, M., Zhou, L., Narcy, J.P., Roux, J., et al. (2007) A critical assessment of Agrobacterium tumefaciens-mediated transformation as a tool for pathogenicity gene discovery in the phytopathogenic fungus Leptosphaeria maculans. Fungal Genet Biol 44: 123-138.
3. Brunaud, V., Balzergue, S., Dubreucq, B., Aubourg, S., Samson, F., Chauvin, S., et al. (2002) T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertions site. EMBO Rep 3: 1152-1157.
4. Bundock, P., Dulk-Ras, A., Beijersbergen, A., and Hooykaas, P.J. (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14: 3206-3214.
5. Bundock, P., van Atticum, H., Dulk-Ras, A., and Hooykaas, P.J. (2002) Insertional mutagenesis in yeast using T-DNA from Agrobacterium tumefaciens. Yeast 19: 529-536.
6. Chen, X., Stone, M., Schlagnhaufer, C., and Romaine, C.P. (2000) A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Appl Environ Micorbiol 66: 4510-4513.
7. Combier, J.P., Melayah, D., Raffier, C., Gay, G., and Marmeisse, R. (2003) Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum. FEMS Microbiol Lett 220: 141-148.
8. De Block, M., and Debrouwer, D. (1991) Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium infection are mainly integrated at the same locus. Theor Appl Genet 82: 257-263.
9. De Groot, M.J., Bundock, P., Hooykaas, P.J., and Beijersbergen, A.G. (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16: 839-842.
10. De Neve, M., de Bruck, S., Jacobs, A., van Montagu, M., and Depicker, A. (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs. Plant J 11: 15-29.
11. Di Battista, C., Selosse, M.A., Bouchard, D., Stenström, E., and Le Tacon, F. (1996) Variation in symbiotic efficiency, phenotypic characters and ploidy level among different isolates of the ectomycorrhizal basidiomycete Laccaria bicolor strain S238. Mycol Res 100: 1315-1324.
12. Duplessis, S., Tagu, D., and Martin, F. (2002) Living together underground: a molecular glimpse of the ectomycorrhizal symbiosis. In Molecular Biology of Fungal development. Mycology Series. Osiewaccz, H.D. (ed.). New York, USA: Dekker & Dekker, pp. 297-323.
13. Duplessis, S., Courty, P., Tagu, D., and Martin, F. (2005) Transcript patterns associated with ectomycorrhiza development in Eucalyptus globulus and Pisolithus microcarpus. New Phytol 165: 599-611.
14. Elliott, C.E., and Howlett, B.J. (2006) Overexpression of a 3-ketoacyl-CoA-thiolase in Leptosphaeria maculans causes reduced pathogenicity on Brassica napus. Mol Plant Microbe Interact 19: 588-596.
15. Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T. (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271-282.
16. Idnurum, A., Reedy, J.L., Nuusbaum, J.C., and Heitman, J. (2004) Cryptococcus neoformans virulence gene discovery through insertional mutagenesis. Eukaryot Cell 3: 420-429.
17. Johansson, T., Le Quéré, A., Ahren, D., Söderström, B., Erlandsson, R., Lundeberg, J., et al. (2004) Transcriptional responses of Paxillus involutus and Betula pendula during formation of ectomycorrhizal root tissue. Mol Plant Microbe Interact 17: 202-215.
18. Kemppainen, M., Circosta, A., Tagu, D., Martin, F., and Pardo, A.G. (2005) Agrobacterium-mediated transformation of the ectomycorrhizal symbiont Laccaria bicolor S238N. Mycorrhiza 16: 19-22.
19. Kim, S.R., Lee, J., Jun, S.H., Park, S., Kang, H.G., Kwon, S., and An, G. (2003) Transgene structures in T-DNA inserted rice plants. Plant Mol Biol 52: 761-773.
20. Kumar, S., and Fladung, M. (2000) Transgene repeats in aspen: molecular characterization suggest simultaneous integration of independent T-DNAs into receptive hotspots in the host genome. Mol Genet Genomics 264: 20-28.
21. Kunik, T., Tzfira, T., Kapulnik, Y., Gafni, Y., Dingwall, C., and Citovsky, V. (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc Natl Acad Sci USA 98: 1871-1876.
22. Laurent, P., Voilet, C., Tagu, D., de Carvalho, D., Nehls, U., De Bellis, R., et al. (1999) A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius. Mol Plant Microbe Interact 12: 862-871.
23. LeClerque, A., Wang, H., Abschutz, A., Chen, S., Mitina, G.V., Zimmerman, G., and Schairer, H.U. (2004) Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana. Curr Genet 45: 111-119.
24. Martin, F., Kohler, A., and Duplessis, S. (2007) Living in harmony in the wood underground: ectomycorrhizal genomics. Curr Opin Plant Biol 10: 204-210.
25. Martin, F., Aerts, A., Ahnén, D., Brun, A., Danchin, E.G.J., Duchaussoy, F., et al. (2008) The genome of Laccaria biocolor provides insights into mycorrhizal symbiosis. Nature 452: 88-92.
26. Mayerhofer, R., Koncz-Kalman, Z., Nawrath, C., Bakkeren, G., Crameri, A., and Angelis, K. (1991) T-DNA integration: a mode of illegimate recombination in plants. EMBO J 10: 697-704.
27. Meng, Y., Patel, G., Heist, M., Betts, M., Tucker, S.L., Galadima, N., et al. (2007) A systematic analysis of T-DNA insertion events in Magnaporthe oryzae. Fungal Genet Biol 44: 1050-1064.
28. Michielse, C.B., Hooykaas, P.J., Hondel, C.A.M., and Ram, A.F.J. (2005) Agrobacterium-mediated transformation as a tool for functional genomics in fungi. Curr Genet 48: 1-17.
29. Offringa, R., de Groot, M.J.A., Haagsman, H.J., Does, M.P., Elzner, P.J.M., and Hooykaas, P.J. (1990) Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation. EMBO J 9: 3077-3084.
30. Pardo, A.G., Hanif, M., Raudaskoski, M., and Gorfer, M. (2002) Genetic transformation of ectomycorrhizal fungi mediated by Agrobacterium tumefaciens. Mycol Res 106: 132-137.
31. Pelczar, P., Kalck, V., Gomes, D., and Hohn, B. (2004) Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells. EMBO Rep 6: 632-637.
32. Peter, M., Courty, P., Kohler, A., Delaruelle, C., Martin, D., Tagu, D., et al. (2003) Analysis of expressed sequence tags from the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus. New Phytol 159: 117-129.
33. Podila, G., Zheng, J., Balasubramanian, S., Sundaram, S., Hieremath, S., Brand, J., and Hymes, M. (2002) Molecular interactions in ectomycorrhizas: identification of fungal genes involved in early symbiotic interactions between Laccaria bicolor and red pine. Plant and Soil 244: 117-128.
34. Rho, H.S., Kang, S., and Lee, Y.H. (2001) Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus Magnaporthe grisea. Mol Cells 12: 407-411.
35. Rogers, C.W., Challen, M.P., Green, J.R., and Whipps, J.M. (2004) Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus Coniothyrium minitans. FEMS Microbiol Lett 241: 207-214.
36. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory.
37. Tinland, B., Schoumacher, F., Cloeckler, V., Bravo-Angel, A.M., and Hohn, B. (1995) The Agrobacterium tumefaciens virulence protein D2 is responsible for precise integration of T-DNA into the plant genome. EMBO J 14: 3585-3595.
38. Tsuji, G., Fujii, S., Fujihara, N., Hirose, C., Tsuge, S., Shiraishi, T., and Kubo, Y. (2003) Agrobacterium tumefaciens-mediated transformation for random insertional mutagenesis in Colletotrichum lagenarium. J Gen Plant Pathol 69: 230-239.
39. Tuskan, G.A., DiFazio, S., Jansson, S., Bohlmann, J., Grigoriev, I., Hellsten, U., et al. (2006) The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 1596-1604.
40. Tzfira, T., Li, J., Lacroix, B., and Citovsky, V. (2004) Agrobacterium T-DNA integration: molecules and models. Trends Genet 20: 375-383.
41. Van Attikum, H., Bundock, P., and Hooykaas, P.J. (2001) Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration. EMBO J 20: 6550-6558.
42. Voiblet, C., Duplessis, S., Encelot, N., and Martin, F. (2001) Title Identification of symbiosis-regulated genes in Eucalyptus globulus-Pisolithus tinctorius ectomycorrhiza by differential hybridization of arrayed cDNAs. Plant J 25: 181-191.
43. Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., et al. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.