Genome-wide insertional mutagenesis of Arabidopsis thaliana

Science

Volumn 301, Issue 5633, 2003, Pages 653-657

  Alonso, José M ^a,d   Stepanova, Anna N ^a,d   Leisse, Thomas J ^a   Kim, Christopher J ^a   Chen, Huaming ^a   Shinn, Paul ^a   Stevenson, Denise K ^a   Zimmerman, Justin ^a   Barajas, Pascual ^a   Cheuk, Rosa ^a   Gadrinab, Carmelita ^a   Heller, Collen ^a   Jeske, Albert ^a   Koesema, Eric ^a   Meyers, Cristina C ^a   Parker, Holly ^a   Prednis, Lance ^a   Ansari, Yasser ^a   Choy, Nathan ^a   Deen, Hashim ^a   Geralt, Michael ^a   Hazari, Nisha ^a   Hom, Emily ^a   Karnes, Meagan ^a   Mulholland, Celene ^a   Ndubaku, Ral ^a   Schmidt, Ian ^a   Guzman, Plinio ^a   Aguilar Henonin, Laura ^a   Schmid, Markus ^a,e   Weigel, Detlef ^a,e   Carter, David E ^b   Marchand, Trudy ^b   Risseeuw, Eddy ^b   Brogden, Debra ^b   Zeko, Albana ^b   Crosby, William L ^b   Berry, Charles C ^c   Ecker, Joseph R ^a   more..

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d NORTH CAROLINA STATE UNIVERSITY   (United States)

^e MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENE TRANSFER; GENES; HORMONES; PLANTS (BOTANY);

MUTATIONS;

MUTAGENESIS;

DNA; ETHYLENE; PHYTOHORMONE;

DNA; GENOME;

ARABIDOPSIS; ARTICLE; DNA INTEGRATION; GENE EXPRESSION REGULATION; GENE INSERTION; GENE LOCATION; GENE TRANSFER; GENETIC ANALYSIS; GENOME; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RHIZOBIUM;

3' UNTRANSLATED REGIONS; 5' UNTRANSLATED REGIONS; ALLELES; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE COMPOSITION; CHROMOSOMES, PLANT; DNA, BACTERIAL; DNA, PLANT; ETHYLENES; EXONS; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GENOME, PLANT; INTRONS; MUTAGENESIS, INSERTIONAL; MUTATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; RHIZOBIUM;

AGROBACTERIUM; ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA; INSERTION SEQUENCES;

EID: 0042768158     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1086391     Document Type: Article

References (28)

1. Arabidopsis Genome Initiative, Nature 408, 796 (2000).
2. P. Ross-Macdonald et al., Nature 402, 413 (1999).
3. E. A. Winzeler et al., Science 285, 901 (1999).
4. G. Giaever et al., Nature 418, 387 (2002).
5. S. Parinov, V. Sundaresan, Curr. Opin. Biotechnol. 11, 157 (2000).
6. M. Gong, Y. S. Rong, Curr. Opin. Genet. Dev. 13, 215 (2003).
7. K. Ashrafi et al., Nature 421, 268 (2003).
8. R. S. Kamath et al., Nature 421, 231 (2003).
9. G. J. Hannon, Nature 418, 244 (2002).
10. C. I. Bargmann, Genome Biol. 2, REVIEWS1005 (2001).
11. S. V. Wesley et al., Plant J. 27, 581 (2001).
12. R. S. Kamath, M. Martinez-Campos, P. Zipperlen, A. G. Fraser, J. Ahringer, Genome Biol. 2, RESEARCH0002 (2001).
13. Materials and methods are available as supporting material on Science Online.
14. J. McElver et al., Genetics 159, 1751 (2001).
15. P. J. Krysan, J. C. Young, M. R. Sussman, Plant Cell 11, 2283 (1999).
16. J. M. Alonso et al., unpublished observations.
17. W. Haupt, T. C. Fischer, S. Winderl, P. Fransz, R. A. Torres-Ruiz, Plant J. 27, 285 (2001).
18. T. Tzfira, V. Citovsky, Trends Cell Biol. 12, 121 (2002).
19. T. Tzfira, V. Citovsky, Mol. Plant Pathol. 1, 201 (2000).
20. A. R. W. Schroder et al., Cell 110, 521 (2002).
21. N. Bechtold et al., Genetics 155, 187S (2000).
22. C. Desfeux, S. J. Clough, A. F. Bent. Plant Physiol. 123, 895 (2000).
23. K. L. Wang, H. Li, J. R. Ecker, Plant Cell 14 (suppl.), S131 (2002)
24. R. Solano, A. Stepanova, Q. Chao, J. R. Ecker, Genes Dev. 12, 3703 (1998)
25. See http://signal.salk.edu
26. H. Chen, J. R. Ecker, unpublished observations.
27. P. Guzman, J. Ecker, Plant Cell 2, 513 (1990)
28. We thank A. Hamilton and members of the Ecker laboratory for critical reading of the manuscript and stimulating discussions. J.M.A. was in part supported by a postdoctoral fellowship from the Spanish Ministerio de Educación y Ciencia. A.N.S. was a recipient of a predoctoral fellowship from the Department of Energy (ER20162-A0003). The W.L.C. laboratory was supported by a grant from the Canadian National Research Council Vice-President's fund. P. G. was on a sabbatical recess from Cinvestav-Irapuato, Mexico, and was supported in part by a fellowship from CONACYT, Mexico. We thank K. Davis and colleagues at Paradigm Genetics for their assistance in bulking up low-seed T1 lines. This work was supported by an NSF Arabidopsis 2010 project grant (MC8 0115103) and Department of Energy DE-FG03-00ER15113 awarded to J.R.E. Seeds for all the T-DNA lines are publicly available from The Arabidopsis Biological Resource Center at Ohio State University (http://arabidopsis.org/abrc/). Affymetrix microarray expression data has been deposited in the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). GEO accessions: GSM8467 through GSM8478.