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Volumn 130, Issue 36, 2008, Pages 12095-12101

Turn-on fluorescence sensing of nucleoside polyphosphates using a xanthene-based Zn(II) complex chemosensor

Author keywords

[No Author keywords available]

Indexed keywords

BINDING SITES; COORDINATION REACTIONS; LIGHT EMISSION; NEGATIVE IONS; PHOSPHATES; ZINC; ZINC COMPOUNDS;

EID: 51749096410     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja803262w     Document Type: Article
Times cited : (293)

References (63)
  • 42
    • 20344404752 scopus 로고    scopus 로고
    • Reports of the μ-oxo-dizinc structure in natural enzymes and artificial enzyme mimics: Matsufuji, K.; Shiraishi, H.; Miyasato, Y.; Shiga, T.; Ohba, M.; Yokoyama, T.; Okawa, H. Bull. Chem. Soc. Jpn. 2005, 78, 851.
    • (a) Reports of the μ-oxo-dizinc structure in natural enzymes and artificial enzyme mimics: Matsufuji, K.; Shiraishi, H.; Miyasato, Y.; Shiga, T.; Ohba, M.; Yokoyama, T.; Okawa, H. Bull. Chem. Soc. Jpn. 2005, 78, 851.
  • 46
    • 51749096172 scopus 로고    scopus 로고
    • In the binding complex with the nucleoside triphosphate such as ATP, we propose that the β- and γ-phosphate groups interact with the two Zn(II)-Dpa sites of 1-2Zn(II) as shown in Figure 5. This is speculated by a 31P NMR study (0.5 mM in 50 mM HEPES, pH 7.4) in which the peaks corresponding to β- and γ-phosphate of ATP observed at-20.7 and-5.9 ppm are shifted downfield at-19.2 and-5.2 ppm, respectively, upon addition of 1 equiv of 1-2 Zn(II) while the peak corresponding to the α-phosphate observed at-9.6 ppm only slightly shifted upheld at-9.9 ppm. Such a two-point binding mode involving the two phosphate groups may reasonably explain the strong binding affinity of 1-2Zn(II) for the nucleoside polyphosphates
    • 31P NMR study (0.5 mM in 50 mM HEPES, pH 7.4) in which the peaks corresponding to β- and γ-phosphate of ATP observed at-20.7 and-5.9 ppm are shifted downfield at-19.2 and-5.2 ppm, respectively, upon addition of 1 equiv of 1-2 Zn(II) while the peak corresponding to the α-phosphate observed at-9.6 ppm only slightly shifted upheld at-9.9 ppm. Such a two-point binding mode involving the two phosphate groups may reasonably explain the strong binding affinity of 1-2Zn(II) for the nucleoside polyphosphates.
  • 58
    • 34250807959 scopus 로고    scopus 로고
    • Recent examples of the xanthene-based fluorogenic chemosensors: Kenmoku, S.; Urano, Y.; Kojima, H.; Nagano, T. J. Am. Chem. Soc. 2007, 129, 7313.
    • (a) Recent examples of the xanthene-based fluorogenic chemosensors: Kenmoku, S.; Urano, Y.; Kojima, H.; Nagano, T. J. Am. Chem. Soc. 2007, 129, 7313.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.