Phosphoprotein-selective recognition and staining in SDS-PAGE by bis-Zn(II)-dipycolylamine-appended anthracene

Chemistry Letters

Volumn 33, Issue 8, 2004, Pages 1024-1025

  Ojida, Akio ^a   Kohira, Takahiro ^a   Hamachi, Itaru ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINE; ANTHRACENE DERIVATIVE; FLUORESCENT DYE; PHOSPHOPROTEIN;

ARTICLE; BIOSENSOR; FLUORESCENCE; GEL ELECTROPHORESIS; MOLECULAR RECOGNITION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS;

EID: 8844270982     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2004.1024     Document Type: Article

References (18)

1. a) B. M. Sefton and T. Hunter, "Protein Phosphorylation," Academic Press, New York (1998).
2. b) L. N. Johnson and R. J. Lewis, Chem. Rev., 101, 2209 (2001).
3. c) T. Pawson, M. Raina, and P. Nash, FEBS Lett., 513, 2 (2002).
4. A. Ojida, Y. Mito-oka, M. Inoue, and I. Hamachi, J. Am. Chem. Soc., 124, 6256 (2002).
5. A. Ojida, M. Inoue, and I. Hamachi, J. Am. Chem. Soc., 125, 10184 (2003).
6. A. Ojida, Y. Mito-oka, K. Sada, and I. Hamachi, J. Am. Chem. Soc., 126, 2454 (2004).
7. max = 360 nm) equipped with CCD camera.
8. J. Tauzin, L. Miclo, S. Roth, D. Mollé, and J.-L. Gaillard, Int. Dairy J., 13, 15 (2003).
9. P. E. Stein, A. G. W. Leslie, J. T. Finch, and R. W. Carrell, J. Mo. Biol., 221, 941 (1991).
10. 2 solution (500 μM) instead of distilled water after staining improved the detection selectivity. This is probably due to that the excess of Zn(II) may prevent the decomplexation of Zn(II) from 1 so as to suppress the nonspecific hydrophobic adsorption of the Zn(II)-unbounded 1 with protein during the washing process.
11. Dephospho-α-casein purchased from Sigma-Aldrich is at least 80% dephosphorylated, meaning that 1-2 phosphate residues are remained in dephospho-α-casein.
12. H. E. Swaissgood, "Developments in Dairy Chemistry-1 Proteins," Applied Science, London (1982).
13. The saturation of fluorescence enhancement is observed nearly at the point of [protein] / [1] = 2.5 in the case of the titration of 1 with dephospho-α-casein (1-2P).
14. a) T. H. Steinberg, B. J. Agnew, K. R. Gee, W.-Y. Leung, T. Goodman, B. Schulenberg, J. Hendrickson, J. M. Beechem, R. P. Haugland, and W. F. Patton, Proteomics, 3, 1128 (2003).
15. b) B. Schulenberg, R. Aggeler, J. M. Beechem, R. A. Capaldi, and W. F. Patton, J. Biol. Chem., 278, 27251 (2003).
16. c) K. Martin, T. H. Steinberg, T. Goodman, B. Schulenberg, J. A. Kilgore, K. R. Gee, J. M. Beechem, and W. F. Patton, Comb. Chem. High Throughput Screening, 6, 331 (2003).
17. a) D. E. Kalume, H. Molina, and A. Pandey, Curr. Opin. Chem. Biol., 7, 64 (2003).
18. b) M. Mann, S.-E. Ong, M. Gronborg, H. Steen, O. N. Jensen, and A. Pandey, Trends Biotechnol., 20, 261 (2002).