Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe

Journal of the American Chemical Society

Volumn 128, Issue 51, 2006, Pages 16476-16477

  Leevy, W Matthew ^a,b   Gammon, Seth T ^d   Jiang, Hua ^a,e   Johnson, James R ^a   Maxwell, Dustin J ^d   Jackson, Erin N ^d   Marquez, Manuel ^c,f,g   Piwnica Worms, David ^d   Smith, Bradley D ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

^b Philip Morris USA   (United States)

^c Research Center   (United States)

^d WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e INSTITUTE OF CHEMISTRY   (China)

^f NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^g Arizona State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL INFECTION; CONTROLLED STUDY; FLUORESCENCE; GRAM POSITIVE BACTERIUM; ILLUMINATION; IMAGING SYSTEM; IN VIVO STUDY; MOLECULAR PROBE; MOUSE; NONHUMAN; NUDE MOUSE; STAPHYLOCOCCUS AUREUS;

ANIMALS; DISEASE MODELS, ANIMAL; FLUORESCENT DYES; MICE; MOLECULAR PROBES; MOLECULAR STRUCTURE; ORGANOMETALLIC COMPOUNDS; PICOLINES; SENSITIVITY AND SPECIFICITY; SPECTROSCOPY, NEAR-INFRARED; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; TOMOGRAPHY, OPTICAL;

EID: 33845943492     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0665592     Document Type: Article

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19. The in vivo imaging data reported in this paper was acquired with the Cy7 filter set recommended by the manufacturer. Efforts to optimize the filter set for probe 1 are ongoing.
20. In vitro measurements of minimum inhibitory concentration (MIC) showed no inhibition of S. aureus growth by probe 1 or fluorophore 2 at the maximum tested concentrations of 100 and 400 μM, respectively. Furthermore, fluorescence microscopy of S. aureus cells grown to confluence in the presence of probe 1 showed no time-dependent change in probe staining intensity or staining location (bacterial cell periphery).
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